Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free (AB232021)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Thymidylate Synthase with purified ab108995 at 1/50 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108995).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free (AB232021)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Thymidylate Synthas with Purified ab108995 at 1 : 100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100 ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108995).

• IP

Lab

Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free (AB232021)

ab108995 (purified) at 1 : 30 dilution (2μg) immunoprecipitating Thymidylate Synthase in Jurkat whole cell lysate.

Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate,10μg
Lane 2 (+) : ab108995 & Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108995 in Jurkat whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108995).

All lanes:

Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (<a href='/en-us/products/primary-antibodies/thymidylate-synthase-antibody-epr4545-ab108995'>ab108995</a>)

Predicted band size: 36 kDa

false

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free (AB232021)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling Thymidylate Synthase with purified ab108995 at 1 : 100 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108995).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free (AB232021)

Unpurified ab108995 at 1/50 dilution, staining Thymidylate Synthase in Human ovary carcinoma Immunohistochemistry, Paraffin-embedded tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108995).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.