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Rabbit Recombinant Monoclonal Thymidylate Synthase antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Cat, Dog, Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human TYMS.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383), expandable thumbnail
  • Western blot - Anti-Thymidylate synthase antibody [HL1236] (AB308383), expandable thumbnail
  • Western blot - Anti-Thymidylate synthase antibody [HL1236] (AB308383), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human TYMS. The exact immunogen used to generate this antibody is proprietary information. Database link P04818

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Expected
Tested
Tested
Mouse
Expected
Expected
Tested
Rat
Expected
Expected
Tested
Cat
Tested
Expected
Tested
Dog
Tested
Expected
Tested

Tested
Tested

Species
Cat, Dog
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

-

Expected
Expected

Species
Cat, Dog, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Cat
Dilution info
1/1000
Notes

-

Species
Dog
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Target data

Function

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Alternative names

TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase

Recommended products

Rabbit Recombinant Monoclonal Thymidylate Synthase antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Cat, Dog, Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human TYMS.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human TYMS. The exact immunogen used to generate this antibody is proprietary information. Database link P04818
Carrier free
Yes
Clone number
HL1236
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.

Biological function summary

Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.

Pathways

Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.

Associated diseases and disorders

Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (ab308383), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (ab308383)

    Immunohistochemical analysis of paraffin-embedded cat brain tissue labelling Thymidylate synthase with ab308383 at 1/100 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (ab308383), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (ab308383)

    Immunohistochemical analysis of paraffin-embedded Dog intestin and colon tissue labelling Thymidylate synthase with ab308383 at 1/100 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (ab308383), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (ab308383)

    Immunohistochemical analysis of paraffin-embedded dog brain tissue labelling Thymidylate synthase with ab308383 at 1/100 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383), expandable thumbnail

    Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383)

    All lanes: Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383) at 1/1000 dilution

    All lanes: HeLa cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 35.7 kDa

  • Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383), expandable thumbnail

    Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383)

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (ab308383) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383) at 1/1000 dilution

    Lane 1: Neuro2A cell lysate at 30 µg

    Lane 2: Rat2 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 35.7 kDa

  • Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383), expandable thumbnail

    Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383)

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (ab308383) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383) at 1/1000 dilution

    Lane 1: MDCK cell lysate at 30 µg

    Lane 2: PG4 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 35.7 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate synthase antibody [HL1236] (ab308383), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate synthase antibody [HL1236] (ab308383)

    For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Thymidylate synthase protein using ab308383 at 1/200 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI. Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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