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AB308383

Anti-Thymidylate synthase antibody [HL1236]

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Rabbit Recombinant Monoclonal Thymidylate Synthase antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Cat, Dog, Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human TYMS.

View Alternative Names

TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

Immunohistochemical analysis of paraffin-embedded cat brain tissue labelling Thymidylate synthase with ab308383 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

Immunohistochemical analysis of paraffin-embedded Dog intestin and colon tissue labelling Thymidylate synthase with ab308383 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

Immunohistochemical analysis of paraffin-embedded dog brain tissue labelling Thymidylate synthase with ab308383 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • WB

Supplier Data

Western blot - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

All lanes:

Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383) at 1/1000 dilution

All lanes:

HeLa cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35.7 kDa

false

Western blot - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • WB

Supplier Data

Western blot - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (ab308383) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383) at 1/1000 dilution

Lane 1:

Neuro2A cell lysate at 30 µg

Lane 2:

Rat2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35.7 kDa

false

Western blot - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • WB

Supplier Data

Western blot - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (ab308383) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Thymidylate synthase antibody [HL1236] (ab308383) at 1/1000 dilution

Lane 1:

MDCK cell lysate at 30 µg

Lane 2:

PG4 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 35.7 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate synthase antibody [HL1236] (AB308383)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate synthase antibody [HL1236] (AB308383)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Thymidylate synthase protein using ab308383 at 1/200 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI. Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1236

Isotype

IgG

Carrier free

Yes

Reacts with

Cat, Dog, Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human TYMS. The exact immunogen used to generate this antibody is proprietary information.

P04818

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Cat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Dog": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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AB288923

Human Thymidylate Synthase ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.
Biological function summary

Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.

Pathways

Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.

Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
See full target information TYMS
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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