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Mouse Monoclonal Thymidylate Synthase antibody. Suitable for ICC, Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TYMS aa 50-200.

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Images

Flow Cytometry - Anti-Thymidylate Synthase antibody [TYMS/1884] (AB224649), expandable thumbnail
  • Immunocytochemistry - Anti-Thymidylate Synthase antibody [TYMS/1884] (AB224649), expandable thumbnail
  • Western blot - Anti-Thymidylate Synthase antibody [TYMS/1884] (AB224649), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [TYMS/1884] (AB224649), expandable thumbnail
  • Protein Array - Anti-Thymidylate Synthase antibody [TYMS/1884] (AB224649), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human TYMS aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P04818

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow CytWBProtein ArrayIHC-P
Human
Tested
Tested
Tested
Expected
Tested
Recombinant full length protein - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg for 106 Cells
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

Primary incubation for 30 minutes at room temperature.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Alternative names

TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase

Recommended products

Mouse Monoclonal Thymidylate Synthase antibody. Suitable for ICC, Flow Cyt, WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TYMS aa 50-200.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human TYMS aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P04818
Clone number
TYMS/1884
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

ab224649 was purified from Bioreactor concentrate by Protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.

Biological function summary

Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.

Pathways

Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.

Associated diseases and disorders

Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Flow Cytometry - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649), expandable thumbnail

    Flow Cytometry - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649)

    Flow Cytometry analysis of PFA-fixed MOLT-4 (human lymphoblastic leukemia cell line) cells labeling Thymidylate Synthase with ab224649 at 2 μg/106 cells followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

  • Immunocytochemistry - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649), expandable thumbnail

    Immunocytochemistry - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649)

    Flow Cytometry analysis of PFA-fixed Ramos (human Burkitt's lymphoma cell line) cells labeling Thymidylate Synthase with ab224649 at 2 μg/mL followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).

  • Western blot - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649), expandable thumbnail

    Western blot - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649)

    All lanes: Western blot - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649) at 2 µg/mL

    All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for Thymidylate Synthase using ab224649 at 2 μg/ml in immunohistochemical analysis.

  • Protein Array - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649), expandable thumbnail

    Protein Array - Anti-Thymidylate Synthase antibody [TYMS/1884] (ab224649)

    ab224649 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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