Rabbit Recombinant Monoclonal Thymosin beta 4 antibody. Suitable for I-ELISA, WB and reacts with Synthetic peptide - Human, Human, Mouse samples.
Images
![Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--western-blot-img446169.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478)")
![Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--western-blot-img446168.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478)")
![Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--western-blot-img446167.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478)")
![Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--western-blot-img446166.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478)")
![Indirect ELISA - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--indirect-elisa-img446250.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Thymosin beta 4 antibody [EPR26609-45] (AB323478)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Not recommended |
| Mouse | Expected | Tested | Not recommended |
| Synthetic peptide - Human | Tested | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 62.5 ng/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Synthetic peptide - Human | Dilution info - | Notes - |
Target data
Function
Plays an important role in the organization of the cytoskeleton (PubMed:10848969, PubMed:1999398). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization (PubMed:10848969, PubMed:1999398). Hemoregulatory peptide AcSDKP. Potent inhibitor of bone marrow derived stem cell differentiation (PubMed:7694679). Acts by inhibits the entry of hematopoietic pluripotent stem cells into the S-phase (By similarity).
Additional Targets
Tmsb4x
Alternative names
TB4X, THYB4, TMSB4, TMSB4X, Thymosin beta-4, T beta-4, Fx
Rabbit Recombinant Monoclonal Thymosin beta 4 antibody. Suitable for I-ELISA, WB and reacts with Synthetic peptide - Human, Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26609-45
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody does not cross-react with human Thymosin beta-4, Y-chromosomal and Thymosin beta-10.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--western-blot-img446169.jpg "Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)")
Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)
Thymosin beta 4 Western blot staining using rabbit Anti-Thymosin beta 4 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1: 37 seconds, Lane 2: 3 seconds
All lanes: Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478) at 1/1000 dilution
Lane 1: C2C12 (mouse myoblast) whole cell lysate at 40 µg
Lane 2: Mouse platelet lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 13 kDa
![Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--western-blot-img446168.jpg "Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)")
Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)
Thymosin beta 4 Western blot staining using rabbit Anti-Thymosin beta 4 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression of Thymosin beta 4 is upregulated in response to PMA treatment. (PMID: 3500230).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478) at 1/1000 dilution
Lane 1: Untreated HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 40 µg
Lane 2: HL-60 treated with 50ng/ml PMA for 72h whole cell lysate at 40 µg
Lane 3: HL-60 treated with 10 uM MG-132 for 4 hours whole cell lysate at 40 µg
Lane 4: HL-60 treated with 50ng/ml PMA for 72h, 10 uM MG-132 was then added for additional 4h whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 13 kDa, 36 kDa
Exposure time: 81s
![Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--western-blot-img446167.jpg "Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)")
Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)
Thymosin beta 4 Western blot staining using rabbit Anti-Thymosin beta 4 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478) at 1/1000 dilution
Lane 1: Human liver cancer tissue lysate at 50 µg
Lane 2: Human breast cancer tissue lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 13 kDa
Exposure time: 59s
![Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--western-blot-img446166.jpg "Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)")
Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)
Thymosin beta 4 Western blot staining using rabbit Anti-Thymosin beta 4 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed molecular weight is greater than the theoretical value due to its hydrophilic nature, which causes slower migration on SDS-PAGE (PMID: 24109178).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478) at 1/1000 dilution
Lane 1: THP-1 (human monocytic leukemia monocyte) transfected with scrambled siRNA control whole cell lysate at 50 µg
Lane 2: THP-1 transfected with siRNA specifically targeting Thymosin beta 4 whole cell lysate at 50 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 13 kDa, 36 kDa
Exposure time: 59s
![Indirect ELISA - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478), expandable thumbnail](https://content.abcam.com/products/images/thymosin-beta-4-antibody-epr26609-45-ab323478--indirect-elisa-img446250.jpg "Indirect ELISA - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)")
Indirect ELISA - Anti-Thymosin beta 4 antibody [EPR26609-45] (ab323478)
Indirect ELISA analysis of ab323478 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.
Antigen: Human Thymosin beta-4; Human Thymosin beta-4, Y-chromosomal; Human Thymosin beta-10.
Antigen concentration: 1000 ng/ml
Downloads
Product protocols
- Visit the general protocols
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