Anti-Thyroglobulin antibody [CPT-R35.3-41]

• WB

Unknown

Western blot - Anti-Thyroglobulin antibody [CPT-R35.3-41] (AB243094)

Blocking and dilution buffer : 5% NFDM/TBST.

Thyroglobulin is a glycoprotein synthesised exclusively by the thyroid follicular cells. The observed molecular weight is consistent with the reported MW (PMID : 14657389).

Negative control : Human kidney.

All lanes:

Western blot - Anti-Thyroglobulin antibody [CPT-R35.3-41] (ab243094) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 10 µg

Lane 2:

Human thyroid tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 305 kDa

Observed band size: 330 kDa

false

Exposure time: 37s

• ELISA

Unknown

ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-41] (AB243094)

50ng CPTAC-39d (1000ng/mL 50 μL per well) was coated onto 96-wells. Serial dilutions (0 0.2 0.9 3.9 15 62 250 1000 ng/mL) of ab243094 (50μL) were incubated with CPTAC-39d in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG (H+L) phosphatase-conjugated secondary antibody (50 μL) into each well and incubating for another 40 minutes. After washing 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

Secondary antibody was used at 1/2500 dilution.