Name: Anti-Thyroglobulin antibody [EPR9730]
SKU: ab156008
Rating: 4 (1 reviews)

Rabbit Recombinant Monoclonal Thyroglobulin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 24 publications.

Images

Western blot - Anti-Thyroglobulin antibody [EPR9730] (AB156008), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Expected	Expected
Rat	Tested	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/50000	Notes -
Species Rat	Dilution info 1/10000 - 1/50000	Notes -
Species Human	Dilution info 1/10000 - 1/50000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250 - 1/500	Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

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13 products for Alternative Product

3 products for Alternative Version

Target data

See full target information Thyroglobulin

Function

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3) (PubMed:17532758, PubMed:32025030). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling in the thyroid follicle lumen (PubMed:32025030). Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream (PubMed:32025030). One dimer produces 7 thyroid hormone molecules (PubMed:32025030).

Alternative names

Thyroglobulin, Tg, TG

Recommended products

Rabbit Recombinant Monoclonal Thyroglobulin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 24 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR9730
Purification technique: Affinity purification Protein A
Specificity: The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Thyroglobulin often abbreviated as Tg is a glycoprotein with a molecular mass of approximately 660 kDa. It is expressed primarily in the thyroid gland particularly within the follicular cells. This protein serves as a precursor for thyroid hormones such as thyroxine (T4) and triiodothyronine (T3). High thyroglobulin levels in the bloodstream can indicate altered thyroid function and clinicians frequently measure these levels for diagnostic purposes. Researchers have developed specific anti-thyroglobulin antibodies to facilitate the study and measurement of this protein in various contexts.

Biological function summary

Thyroglobulin plays a critical role in the synthesis and storage of thyroid hormones. It acts as a scaffold for iodination and hormone synthesis within the colloid of thyroid follicles. Thyroglobulin is not part of a protein complex; rather it undergoes iodination to form hormone precursors that later convert into active hormones. Anti-thyroglobulin antibodies can be used in research or diagnostic labs to detect the presence or concentration of thyroglobulin in serum samples. These antibodies help in understanding the nuances of thyroid hormone biosynthesis and in monitoring thyroid activity.

Pathways

Thyroglobulin is deeply involved in the thyroid hormone synthesis pathway a fundamental part of the endocrine system's operations. This process connects closely with the iodine metabolism pathway as iodine plays an essential role in hormone production. Thyroglobulin interacts indirectly with proteins like thyroid peroxidase which catalyzes iodination of thyroglobulin. The synthesis and release of thyroid hormones proceed through a carefully orchestrated sequence affecting many physiological processes including metabolism growth and development.

Associated diseases and disorders

Thyroglobulin has associations with conditions such as thyroid cancer and autoimmune thyroid diseases. For instance elevated serum thyroglobulin can serve as a tumor marker in differentiated thyroid cancer. Anti-thyroglobulin antibodies are often present in autoimmune thyroid diseases like Hashimoto's thyroiditis where the body's immune system mistakenly targets thyroid proteins. Thyroid peroxidase antibodies are also common in these disorders indicating a shared autoimmune response against thyroid-specific antigens. Understanding these relationships aids in the diagnosis and management of thyroid-related conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution
All lanes: Rat thyroid lysates at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 305 kDa
Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Intracellular Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with purified ab156008 at 1/70 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with Purified ab156008 at 1:100 dilution. Cells were fixed in 100% Methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling Thyroglobulin with Purified ab156008 at 1:500 dilution (1.36 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution
Lane 1: Mouse thyroid lysates at 15 µg
Lane 2: ICR thyroid lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 305 kDa
This image is courtesy of a customer review submitted by Sanjay Gawade.
Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Unpurified ab156008 staining Thyroglobulin in mouse thyroid cells by intracellular flow cytometry. Cells were fixed with formaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/100 in FACS buffer) for 30 minutes at 24°C. An Alexa Fluor^® 647-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Gating Strategy: Epithlial cells. Red line shows unlabeled sample, blue line shows labeled sample.
Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution
All lanes: Human thyroid lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 305 kDa
Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
All lanes: Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution
Lane 1: Mouse thyroid lysate at 10 µg
Lane 2: Rat thyroid lysate at 10 µg
Lane 3: Human thyroid lysate at 10 µg
Secondary
All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 305 kDa
Observed band size: 294-300 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Immunohistochemical analysis of paraffin embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.
Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
Immunofluorescent analysis of TT cells labeling Thyroglobulin with unpurified ab156008 at 1/50.