Anti-Thyroglobulin antibody [EPR9730]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Immunohistochemical analysis of paraffin embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Immunofluorescent analysis of TT cells labeling Thyroglobulin with unpurified ab156008 at 1/50.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling Thyroglobulin with Purified ab156008 at 1 : 500 dilution (1.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Intracellular Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with purified ab156008 at 1/70 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with Purified ab156008 at 1 : 100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

AbReview41903****

Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Unpurified ab156008 staining Thyroglobulin in mouse thyroid cells by intracellular flow cytometry. Cells were fixed with formaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/100 in FACS buffer) for 30 minutes at 24°C. An Alexa Fluor^® 647-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Gating Strategy : Epithlial cells. Red line shows unlabeled sample, blue line shows labeled sample.

This image is courtesy of an Abreview submitted by Sanjay Gawade.

• WB

Lab

Western blot - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution

All lanes:

Human thyroid lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 305 kDa

false

• WB

Lab

Western blot - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution

All lanes:

Rat thyroid lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 305 kDa

false

• WB

Unknown

Western blot - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

All lanes:

Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution

Lane 1:

Mouse thyroid lysate at 10 µg

Lane 2:

Rat thyroid lysate at 10 µg

Lane 3:

Human thyroid lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 305 kDa

Observed band size: 294-300 kDa

false

• WB

Lab

Western blot - Anti-Thyroglobulin antibody [EPR9730] (AB156008)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution

Lane 1:

Mouse thyroid lysates at 15 µg

Lane 2:

ICR thyroid lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 305 kDa

false