Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (AB229449)

This IHC data was generated using the same anti-Thyroglobulin antibody clone EPR9730 in a different buffer formulation (cat# ab156008).

Immunohistochemical analysis of paraffin embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (AB229449)

This ICC/IF data was generated using the same anti-Thyroglobulin antibody clone EPR9730 in a different buffer formulation (cat# ab156008).

Immunofluorescent analysis of TT cells labeling Thyroglobulin with unpurified ab156008 at 1/50.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (AB229449)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling Thyroglobulin with Purified ab156008 at 1 : 500 dilution (1.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab156008).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (AB229449)

Intracellular Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with purified ab156008 at 1/70 dilution (10 �g/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^� 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (AB229449)

Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with Purified ab156008 at 1 : 100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA glycerol and sodium azide (ab156008).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (AB229449)

Unpurified ab156008 staining Thyroglobulin in mouse thyroid cells by intracellular flow cytometry. Cells were fixed with formaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/100 in FACS buffer) for 30 minutes at 24�C. An Alexa Fluor^� 647-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Gating Strategy : Epithlial cells. Red line shows unlabeled sample, blue line shows labeled sample.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab156008).

This image is courtesy of an Abreview submitted by Sanjay Gawade.