Anti-Thyroid Hormone Receptor alpha antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor alpha antibody (AB53729)

ab53729 at 1/50 dilution staining Thyroid Hormone Receptor alpha in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor alpha antibody (AB53729)

ICC/IF image of ab53729 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53729, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ChIP

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ChIP - Anti-Thyroid Hormone Receptor alpha antibody (AB53729)

Chromatin was prepared from C2C12 cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 5μg of ab53729 (red), and 20μl of protein A/G sepharose beads slurry (10μl of sepharose A beads + 10μl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

• WB

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Western blot - Anti-Thyroid Hormone Receptor alpha antibody (AB53729)

The molecular weight observed is consistent with what has been described in the literature.

Lot : 811200345

All lanes:

Western blot - Anti-Thyroid Hormone Receptor alpha antibody (ab53729) at 1/1000 dilution

Lane 1:

HeLa

Lane 2:

HepG2

Lane 3:

CACO2

Lane 4:

SW480

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

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