Rabbit Polyclonal THA antibody. Suitable for ChIP, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human THRA aa 1-100.
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- Reproductive biology and endocrinology : RB&E 21:1082023Thyroid hormone transport and metabolism are disturbed in the placental villi of miscarriage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Yu,Xinghao Feng,Zhongshan Lin,Xuan Li,Shiyue Su,Huiru Cheng,Yuanyuan Yang,Zhaolian WeiPubMed 37968664
- Cells 11:2022Alterations of Lipid Metabolism in the Heart in Spontaneously Hypertensive Rats Precedes Left Ventricular Hypertrophy and Cardiac Dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomasz K Bednarski,Monika K Duda,Pawel DobrzynPubMed 36230994
- Thyroid : official journal of the American Thyroid Association 32:581-5932022Maternal Exercise Before and During Pregnancy Facilitates Embryonic Myogenesis by Enhancing Thyroid Hormone Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Gao,Liang Zhao,Jun Seok Son,Xiangdong Liu,Yanting Chen,Jeanene Marie Deavila,Mei-Jun Zhu,Gordon K Murdoch,Min DuPubMed 35286177
- Journal of applied toxicology : JAT 42:1628-163820222,3',4,4',5-Pentachlorobiphenyl attenuated fast-twitch fibers and fiber size of skeletal muscle via disturbing thyroid hormone signaling and mitochondrial dynamics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoxia Zhu,Yuxin Zou,Xinyu Qi,Yunlu Sheng,Shan Lv,Jing Yu,Xiaodong Wang,Guoxian Ding,Yu DuanPubMed 35411558
- Journal of clinical medicine 10:2021Changes in Thyroid Hormone Signaling Mediate Cardiac Dysfunction in the Tg197 Mouse Model of Arthritis: Potential Therapeutic Implications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLydia Ntari,Polyxeni Mantzouratou,Athanasia Katsaouni,Constantinos Pantos,George Kollias,Iordanis MourouzisPubMed 34884213
- International journal of molecular sciences 22:2021Smoothelin-Like Protein 1 Regulates the Thyroid Hormone-Induced Homeostasis and Remodeling of C2C12 Cells via the Modulation of Myosin Phosphatase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvelin Major,Ilka Keller,Dániel Horváth,István Tamás,Ferenc Erdődi,Beáta LontayPubMed 34638630
- International journal of molecular sciences 22:2020Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam Olichwier et. al.PubMed 33374300
- Journal of trace elements in medicine and biology 47:89-972018Thyroid function alterations attributed to high iodide supplementation in maternal rats and their offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Liang et. al.PubMed 29544812
- Animal science journal = Nihon chikusan Gakkaiho 89:513-5262017Thyroid hormones alter estrous cyclicity and antioxidative status in the ovaries of rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuanwei Wei,Jaafar Sulieman Fedail,Lingfa Kong,Kaizhi Zheng,Chunhua Meng,Mohamed Babo Fadlalla,Fangxiong ShiPubMed 29214681
- PloS one 11:e01678052016Effects of In Utero Thyroxine Exposure on Murine Cranial Suture Growth.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human THRA aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P10827
Reactivity data
Select an application| ChIP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected | Expected |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-5.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Isoform Alpha-1. Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. Isoform Alpha-2. Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action.
Alternative names
EAR7, ERBA1, NR1A1, THRA1, THRA2, THRA, Thyroid hormone receptor alpha, Nuclear receptor subfamily 1 group A member 1, V-erbA-related protein 7, c-erbA-1, c-erbA-alpha, EAR-7
Recommended products
Rabbit Polyclonal THA antibody. Suitable for ChIP, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human THRA aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human THRA aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P10827
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab53729 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thyroid Hormone Receptor alpha (THRA) also known as NR1A1 or c-erbA-1 acts as a nuclear receptor that binds thyroid hormones particularly triiodothyronine (T3). THRA weighs approximately 54 kDa. This receptor exists in various isoforms with the alpha-1 version playing a significant role in mediating thyroid hormone action. THRA is widely expressed in many tissues with high levels in the heart skeletal muscles and brain emphasizing its diverse functional impact.
- Biological function summary
The receptor influences many cellular processes such as metabolism differentiation and development. It often works within a complex composed of other nuclear receptors and coregulators to regulate gene expression upon thyroid hormone binding. By altering transcriptional activity THRA affects cell growth energy metabolism and the proper functioning of vital organs. This regulation is critical during both developmental phases and adult physiological maintenance.
- Pathways
THRA integrates within the thyroid hormone signaling and metabolic regulation pathways. It modulates genes involved in energy metabolism such as uncoupling proteins influencing how cells utilize and store energy. THRA interacts with related proteins like Thyroid Hormone Receptor beta (THRB) collectively orchestrating the intricate control of metabolic processes particularly in response to hormonal signals that adjust body functions based on physiological needs.
- Associated diseases and disorders
Mutations or dysfunction of THRA associates with conditions like RTHalpha (Resistance to Thyroid Hormone alpha) and some metabolic syndromes. RTHalpha manifests with symptoms such as impaired growth and cognitive deficits. THRA's interaction with THRB can exacerbate these issues highlighting their linked contribution to thyroid hormone resistance. Investigating these interactions presents opportunities for understanding and potentially treating related disorders more effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
ChIP - Anti-Thyroid Hormone Receptor alpha antibody (ab53729)
Chromatin was prepared from C2C12 cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 5μg of ab53729 (red), and 20μl of protein A/G sepharose beads slurry (10μl of sepharose A beads + 10μl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Western blot - Anti-Thyroid Hormone Receptor alpha antibody (ab53729)
The molecular weight observed is consistent with what has been described in the literature.
Lot: 811200345
All lanes: Western blot - Anti-Thyroid Hormone Receptor alpha antibody (ab53729) at 1/1000 dilution
Lane 1: HeLa
Lane 2: HepG2
Lane 3: CACO2
Lane 4: SW480
SecondaryAll lanes: Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor alpha antibody (ab53729)
ab53729 at 1/50 dilution staining Thyroid Hormone Receptor alpha in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor alpha antibody (ab53729)
ICC/IF image of ab53729 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53729, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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