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Rabbit Recombinant Monoclonal THA antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-Thyroid Hormone Receptor alpha antibody [EPR7862] - BSA and Azide free (AB250085), expandable thumbnail
  • Western blot - Anti-Thyroid Hormone Receptor alpha antibody [EPR7862] - BSA and Azide free (AB250085), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Isoform Alpha-1. Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. Isoform Alpha-2. Does not bind thyroid hormone and functions as a weak dominant negative inhibitor of thyroid hormone action.

Alternative names

EAR7, ERBA1, NR1A1, THRA1, THRA2, THRA, Thyroid hormone receptor alpha, Nuclear receptor subfamily 1 group A member 1, V-erbA-related protein 7, c-erbA-1, c-erbA-alpha, EAR-7

Recommended products

Rabbit Recombinant Monoclonal THA antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7862
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250085 is the carrier-free version of Anti-Thyroid Hormone Receptor alpha antibody [EPR7862] ab179438.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Thyroid Hormone Receptor alpha (THRA) also known as NR1A1 or c-erbA-1 acts as a nuclear receptor that binds thyroid hormones particularly triiodothyronine (T3). THRA weighs approximately 54 kDa. This receptor exists in various isoforms with the alpha-1 version playing a significant role in mediating thyroid hormone action. THRA is widely expressed in many tissues with high levels in the heart skeletal muscles and brain emphasizing its diverse functional impact.

Biological function summary

The receptor influences many cellular processes such as metabolism differentiation and development. It often works within a complex composed of other nuclear receptors and coregulators to regulate gene expression upon thyroid hormone binding. By altering transcriptional activity THRA affects cell growth energy metabolism and the proper functioning of vital organs. This regulation is critical during both developmental phases and adult physiological maintenance.

Pathways

THRA integrates within the thyroid hormone signaling and metabolic regulation pathways. It modulates genes involved in energy metabolism such as uncoupling proteins influencing how cells utilize and store energy. THRA interacts with related proteins like Thyroid Hormone Receptor beta (THRB) collectively orchestrating the intricate control of metabolic processes particularly in response to hormonal signals that adjust body functions based on physiological needs.

Associated diseases and disorders

Mutations or dysfunction of THRA associates with conditions like RTHalpha (Resistance to Thyroid Hormone alpha) and some metabolic syndromes. RTHalpha manifests with symptoms such as impaired growth and cognitive deficits. THRA's interaction with THRB can exacerbate these issues highlighting their linked contribution to thyroid hormone resistance. Investigating these interactions presents opportunities for understanding and potentially treating related disorders more effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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