AB53170

Anti-Thyroid Hormone Receptor beta antibody

Name: Anti-Thyroid Hormone Receptor beta antibody (ab53170) | Abcam
Brand: Abcam Limited
SKU: AB53170

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(2 Publications)

Rabbit Polyclonal Thyroid Hormone Receptor beta antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human THRB.

View Alternative Names

ERBA2, NR1A2, THR1, THRB, Thyroid hormone receptor beta, Nuclear receptor subfamily 1 group A member 2, c-erbA-2, c-erbA-beta

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

ICC/IF image of ab53170 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53170, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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Western blot - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

All lanes:

Western blot - Anti-Thyroid Hormone Receptor beta antibody (ab53170) at 1/500 dilution

Lane 1:

LoVo cell extracts minus immunising peptide

Lane 2:

LoVo cell extracts plus immunising peptide

Predicted band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human THRB. The exact immunogen used to generate this antibody is proprietary information.

P10828

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Thyroid Hormone Receptor beta (THRB) also known as c-erbA-2 and NR1A2 is a nuclear hormone receptor and transcription factor weighing approximately 55 kDa. THRB binds thyroid hormones and plays a central role in moderating gene expression. Expression of this receptor occurs in various tissues with notable presence in the liver brain and heart. As a transcription factor it influences the transcription of specific target genes upon binding to thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3).

Biological function summary

This receptor regulates critical metabolic processes and plays a role in development. THRB functions as part of a larger receptor complex partnering with retinoid X receptor (RXR) to form a heterodimer. This dimer binds to thyroid hormone response elements (TREs) on DNA modulating the expression of genes involved in metabolism thermogenesis and development of the central nervous system. The receptor's activity impacts many key physiological functions ensuring proper growth and metabolic balance.

Pathways

THRB engages significantly in the thyroid hormone signaling pathway and the lipid degradation pathway. It interacts with proteins like the aforementioned RXR in promoting transcriptional activities. In its regulatory capacity THRB helps facilitate lipid metabolism cholesterol homeostasis and energy management. As part of the thyroid hormone signaling pathway it controls numerous genes aligning with proteins like thyroid peroxidase (TPO) which in turn affect hormone synthesis.

Mutations or malfunctions in THRB relate to conditions such as resistance to thyroid hormone (RTH) and medullary thyroid carcinoma. In RTH inappropriate THRB activity results in altered thyroid hormone action and impaired feedback mechanism. This connects to disrupted signaling involving proteins like thyroglobulin. In medullary thyroid carcinoma THRB alterations can influence tumor progression demonstrating its impact on cellular regulatory mechanisms. Understanding THRB's involvement in these disorders aids in diagnosing and developing targeted therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

See full target information THRB

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Endocrinology 157:3278-92 PubMed27253998

2016

Thyroid Hormone Receptor-β (TRβ) Mediates Runt-Related Transcription Factor 2 (Runx2) Expression in Thyroid Cancer Cells: A Novel Signaling Pathway in Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Frances E Carr,Phillip W L Tai,Michael S Barnum,Noelle E Gillis,Katherine G Evans,Thomas H Taber,Jeffrey H White,Jennifer A Tomczak,Diane M Jaworski,Sayyed K Zaidi,Jane B Lian,Janet L Stein,Gary S Stein

The American journal of pathology 184:230-47 PubMed24215914

2013

The thyroid hormone triiodothyronine controls macrophage maturation and functions: protective role during inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Cristiana Perrotta,Marcella Buldorini,Emma Assi,Denise Cazzato,Clara De Palma,Emilio Clementi,Davide Cervia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thyroid Hormone Receptor beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

Western blot - Anti-Thyroid Hormone Receptor beta antibody (AB53170)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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