AB5622

Anti-Thyroid Hormone Receptor beta antibody

Name: Anti-Thyroid Hormone Receptor beta antibody (ab5622) | Abcam
Brand: Abcam Limited
SKU: AB5622
Price: 535 USD
Availability: InStock

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(36 Publications)

Rabbit Polyclonal Thyroid Hormone Receptor beta antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human THRB aa 50-100.

View Alternative Names

ERBA2, NR1A2, THR1, THRB, Thyroid hormone receptor beta, Nuclear receptor subfamily 1 group A member 2, c-erbA-2, c-erbA-beta

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody (AB5622)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody (AB5622)

ab5622 staining Thyroid Hormone Receptor in Human colon tissue sections (right) compared to negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/20) for 1 hour at 37°C. A HRP-conjugated secondary antibody was used for detection.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody (AB5622)

ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Human thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody (AB5622)

ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Rat thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1 : 200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human THRB aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P10828

Specificity

This antibody does not detect TR alpha-1 or TRv alpha-2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Thyroid Hormone Receptor beta (THRB) also known as c-erbA-2 and NR1A2 is a nuclear hormone receptor and transcription factor weighing approximately 55 kDa. THRB binds thyroid hormones and plays a central role in moderating gene expression. Expression of this receptor occurs in various tissues with notable presence in the liver brain and heart. As a transcription factor it influences the transcription of specific target genes upon binding to thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3).

Biological function summary

This receptor regulates critical metabolic processes and plays a role in development. THRB functions as part of a larger receptor complex partnering with retinoid X receptor (RXR) to form a heterodimer. This dimer binds to thyroid hormone response elements (TREs) on DNA modulating the expression of genes involved in metabolism thermogenesis and development of the central nervous system. The receptor's activity impacts many key physiological functions ensuring proper growth and metabolic balance.

Pathways

THRB engages significantly in the thyroid hormone signaling pathway and the lipid degradation pathway. It interacts with proteins like the aforementioned RXR in promoting transcriptional activities. In its regulatory capacity THRB helps facilitate lipid metabolism cholesterol homeostasis and energy management. As part of the thyroid hormone signaling pathway it controls numerous genes aligning with proteins like thyroid peroxidase (TPO) which in turn affect hormone synthesis.

Mutations or malfunctions in THRB relate to conditions such as resistance to thyroid hormone (RTH) and medullary thyroid carcinoma. In RTH inappropriate THRB activity results in altered thyroid hormone action and impaired feedback mechanism. This connects to disrupted signaling involving proteins like thyroglobulin. In medullary thyroid carcinoma THRB alterations can influence tumor progression demonstrating its impact on cellular regulatory mechanisms. Understanding THRB's involvement in these disorders aids in diagnosing and developing targeted therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

See full target information THRB

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 45:2115-2133 PubMed40446811

2025

Brain-targeted mild hypothermia ameliorates ischemic brain injury and promotes stroke recovery in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Junxuan Lyu,Liqiang Liu,Mengyuan Guo,Sicheng Li,Wei Su,Jia Liu,Xunming Ji

Scientific reports 14:19522 PubMed39174599

2024

AUTS2 disruption causes neuronal differentiation defects in human cerebral organoids through hyperactivation of the WNT/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuangzhuang Geng,Yen Teng Tai,Qiang Wang,Zhonghua Gao

Life science alliance 7: PubMed38609183

2024

Nr1h4 and Thrb ameliorate ER stress and provide protection in the MPTP mouse model of Parkinson's.

Applications

Unspecified application

Species

Unspecified reactive species

Nancy Ahuja,Shalini Gupta,Rashmi Arora,Ella Bhagyaraj,Drishti Tiwari,Sumit Kumar,Pawan Gupta

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38148154

2023

Distinctive In Vitro Phenotypes in iPSC-Derived Neurons From Patients With Gain- and Loss-of-Function Developmental and Epileptic Encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Mao,Cristiana Mattei,Ben Rollo,Sean Byars,Claire Cuddy,Geza Berecki,Jacqueline Heighway,Svenja Pachernegg,Trevelyan Menheniott,Danielle Apted,Linghan Jia,Kelley Dalby,Alex Nemiroff,Saul Mullen,Christopher A Reid,Snezana Maljevic,Steven Petrou

Reproductive biology and endocrinology : RB&E 21:108 PubMed37968664

2023

Thyroid hormone transport and metabolism are disturbed in the placental villi of miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Yu,Xinghao Feng,Zhongshan Lin,Xuan Li,Shiyue Su,Huiru Cheng,Yuanyuan Yang,Zhaolian Wei

Cell death & disease 14:474 PubMed37500624

2023

Statins suppress cell-to-cell propagation of α-synuclein by lowering cholesterol.

Applications

Unspecified application

Species

Unspecified reactive species

Joo-Ok Min,Hoang-Anh Ho,Wonjae Lee,Byung Chul Jung,Sung Jun Park,Seokjoong Kim,Seung-Jae Lee

eLife 12: PubMed37272619

2023

Microcephaly-associated protein WDR62 shuttles from the Golgi apparatus to the spindle poles in human neural progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Dell'Amico,Marilyn M Angulo Salavarria,Yutaka Takeo,Ichiko Saotome,Maria Teresa Dell'Anno,Maura Galimberti,Enrica Pellegrino,Elena Cattaneo,Angeliki Louvi,Marco Onorati

Life sciences 311:121158 PubMed36370870

2022

Sfrp4 expression in thyroxine treated calvarial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emily L Durham,Zachary J Grey,Laurel Black,R Nicole Howie,Jeremy L Barth,Beth S Lee,James J Cray

CNS neuroscience & therapeutics 29:129-139 PubMed36341958

2022

Selective brain hypothermia attenuates focal cerebral ischemic injury and improves long-term neurological outcome in aged female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liqiang Liu,Jia Liu,Ming Li,Junxuan Lyu,Wei Su,Shejun Feng,Xunming Ji

Annals of neurology 92:45-60 PubMed35467038

2022

A Role for Insulin-like Growth Factor 1 in the Generation of Epileptic Spasms in a murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos J Ballester-Rosado,John T Le,Trang T Lam,Carrie A Mohila,Sandi Lam,Anne E Anderson,James D Frost,John W Swann

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Thyroid Hormone Receptor beta antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody (AB5622)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody (AB5622)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody (AB5622)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

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