AB180612

Anti-Thyroid Hormone Receptor beta antibody - N-terminal

Name: Anti-Thyroid Hormone Receptor beta antibody - N-terminal (ab180612) | Abcam
Brand: Abcam Limited
SKU: AB180612
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal Thyroid Hormone Receptor beta antibody. N-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human THRB aa 1-250.

View Alternative Names

ERBA2, NR1A2, THR1, THRB, Thyroid hormone receptor beta, Nuclear receptor subfamily 1 group A member 2, c-erbA-2, c-erbA-beta

1 Images

Supplier Data

Western blot - Anti-Thyroid Hormone Receptor beta antibody - N-terminal (AB180612)

Blocking buffer : 3% nonfat dry milk in TBST

All lanes:

Western blot - Anti-Thyroid Hormone Receptor beta antibody - N-terminal (ab180612) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 25 µg

Lane 2:

U-87MG cell lysate at 25 µg

Lane 3:

Mouse liver tissue lysate at 25 µg

Lane 4:

Mouse eye tissue lysate at 25 µg

Lane 5:

Rat liver tissue lysate at 25 µg

Lane 6:

Rat eye tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat AntiRabbit IgG (H+L)

Predicted band size: 52 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human THRB aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P10828

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Thyroid Hormone Receptor beta (THRB) also known as c-erbA-2 and NR1A2 is a nuclear hormone receptor and transcription factor weighing approximately 55 kDa. THRB binds thyroid hormones and plays a central role in moderating gene expression. Expression of this receptor occurs in various tissues with notable presence in the liver brain and heart. As a transcription factor it influences the transcription of specific target genes upon binding to thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3).

Biological function summary

This receptor regulates critical metabolic processes and plays a role in development. THRB functions as part of a larger receptor complex partnering with retinoid X receptor (RXR) to form a heterodimer. This dimer binds to thyroid hormone response elements (TREs) on DNA modulating the expression of genes involved in metabolism thermogenesis and development of the central nervous system. The receptor's activity impacts many key physiological functions ensuring proper growth and metabolic balance.

Pathways

THRB engages significantly in the thyroid hormone signaling pathway and the lipid degradation pathway. It interacts with proteins like the aforementioned RXR in promoting transcriptional activities. In its regulatory capacity THRB helps facilitate lipid metabolism cholesterol homeostasis and energy management. As part of the thyroid hormone signaling pathway it controls numerous genes aligning with proteins like thyroid peroxidase (TPO) which in turn affect hormone synthesis.

Mutations or malfunctions in THRB relate to conditions such as resistance to thyroid hormone (RTH) and medullary thyroid carcinoma. In RTH inappropriate THRB activity results in altered thyroid hormone action and impaired feedback mechanism. This connects to disrupted signaling involving proteins like thyroglobulin. In medullary thyroid carcinoma THRB alterations can influence tumor progression demonstrating its impact on cellular regulatory mechanisms. Understanding THRB's involvement in these disorders aids in diagnosing and developing targeted therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.

See full target information THRB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 6: PubMed34237030

2021

Thyroid hormone receptor β sumoylation is required for thyrotropin regulation and thyroid hormone production.

Applications

Unspecified application

Species

Unspecified reactive species

Sujie Ke,Yan-Yun Liu,Rajendiran Karthikraj,Kurunthachalam Kannan,Jingjing Jiang,Kiyomi Abe,Anna Milanesi,Gregory A Brent

International journal of molecular sciences 22: PubMed33374300

2020

Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Olichwier,Volodymyr V Balatskyi,Marcin Wolosiewicz,James M Ntambi,Pawel Dobrzyn

PloS one 11:e0167805 PubMed27959899

2016

Effects of In Utero Thyroxine Exposure on Murine Cranial Suture Growth.

Applications

IHC-P

Species

Mouse

R Nicole Howie,Emily L Durham,Laurel Black,Grace Bennfors,Trish E Parsons,Mohammed E Elsalanty,Jack C Yu,Seth M Weinberg,James J Cray

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thyroid Hormone Receptor beta antibody - N-terminal

Western blot - Anti-Thyroid Hormone Receptor beta antibody - N-terminal (AB180612)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise