AB203057

Anti-Thyroid Peroxidase/TPO antibody

Name: Anti-Thyroid Peroxidase/TPO antibody (ab203057) | Abcam
Brand: Abcam Limited
SKU: AB203057
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(5 Publications)

Rabbit Polyclonal Thyroid Peroxidase/TPO antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Mouse Thyroid peroxidase aa 350-450 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Thyroid peroxidase, TPO

1 Images

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Thyroid Peroxidase/TPO antibody (AB203057)

MCF-7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Thyroid peroxidase Polyclonal Antibody (ab203057) at 1/100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Synthetic Peptide within Mouse Thyroid peroxidase aa 350-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P35419

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thyroid Peroxidase (TPO) also known as thyroperoxidase is an enzyme with a molecular mass of around 105 kDa. It is expressed in the thyroid gland's follicular cells. TPO plays a mechanical role in the synthesis of thyroid hormones by catalyzing iodination and coupling reactions needed to produce triiodothyronine (T3) and thyroxine (T4). These hormones are essential for regulating metabolism growth and development. The activity of TPO is an important step in thyroid hormone production.

Biological function summary

TPO participates in the thyroid's hormone biosynthesis pathway. It forms a part of the multi-enzyme complex that includes thyroglobulin and iodide. The enzyme facilitates the iodination of tyrosine residues on thyroglobulin and the coupling of iodotyrosines important for creating active thyroid hormones. TPO's enzymatic function is important for maintaining appropriate levels of circulating T3 and T4 impacting various physiological processes in the body.

Pathways

TPO contributes to the thyroid hormone synthesis pathway which is a critical pathway for metabolic regulation. It works in close association with other proteins such as thyroglobulin and sodium/iodide symporter (NIS) to ensure efficient thyroid hormone production. TPO's involvement in these pathways indicates its importance in the endocrine system's normal function and homeostasis.

TPO is often associated with autoimmune thyroid diseases like Hashimoto's thyroiditis and Graves' disease. High TPO antibodies such as anti-TPO antibodies are commonly detected in these conditions indicating an autoimmune response against the thyroid. The presence of high anti-thyroid peroxidase antibodies can lead to the destruction of thyroid tissue often resulting in hypothyroidism. TPO's connection to these diseases highlights its role in autoimmune conditions affecting thyroid function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).

See full target information Thyroid peroxidase

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 14:e1694 PubMed38797942

2024

Targeting miR-31 represses tumourigenesis and dedifferentiation of BRAF-associated thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Peitao Zhang,Lizhao Guan,Wei Sun,Yu Zhang,Yaying Du,Shukai Yuan,Xiaolong Cao,Zhengquan Yu,Qiang Jia,Xiangqian Zheng,Zhaowei Meng,Xingrui Li,Li Zhao

Environmental toxicology 37:2924-2936 PubMed36005737

2022

Di(2-ethylhexyl) phthalate induced thyroid toxicity via endoplasmic reticulum stress: In vivo and in vitro study.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Xu,Tianyang Zhao,Hyonju Ri,Jiaming Ye,Weisen Zhao,Yuezhu Zhang,Lin Ye

Frontiers in immunology 12:637659 PubMed33767707

2021

Activation of Toll-Like Receptor 7 Signaling Pathway in Primary Sjögren's Syndrome-Associated Thrombocytopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Zhang,Jingge Qu,Li Wang,Mengtao Li,Dong Xu,Yan Zhao,Fengchun Zhang,Xiaofeng Zeng

Molecular medicine reports 23: PubMed33649816

2021

Novel insights into di‑(2‑ethylhexyl)phthalate activation: Implications for the hypothalamus‑pituitary‑thyroid axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyu Wu,Wanying Zhang,Yunbo Zhang,Zhen Kang,Xinxiunan Miao,Xiaolin Na

Scientific reports 8:4004 PubMed29507327

2018

Generation and characterization of a hypothyroidism rat model with truncated thyroid stimulating hormone receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Jianqiang Yang,Ning Yi,Junhui Zhang,Wen He,Di He,Wanwan Wu,Shuyang Xu,Feng Li,Guoping Fan,Xianmin Zhu,Zhigang Xue,Wensheng Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thyroid Peroxidase/TPO antibody

Flow Cytometry - Anti-Thyroid Peroxidase/TPO antibody (AB203057)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise