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Rabbit Recombinant Monoclonal Thyroid Peroxidase/TPO antibody. Suitable for mIHC, IHC-P, IP, WB and reacts with Human samples. Cited in 8 publications.


Images

Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (AB109383), expandable thumbnail
  • Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (AB109383), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (AB109383), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (AB109383), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-PIPWB
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).

Alternative names

Thyroid peroxidase, TPO

Recommended products

Rabbit Recombinant Monoclonal Thyroid Peroxidase/TPO antibody. Suitable for mIHC, IHC-P, IP, WB and reacts with Human samples. Cited in 8 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5380
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Thyroid Peroxidase (TPO) also known as thyroperoxidase is an enzyme with a molecular mass of around 105 kDa. It is expressed in the thyroid gland's follicular cells. TPO plays a mechanical role in the synthesis of thyroid hormones by catalyzing iodination and coupling reactions needed to produce triiodothyronine (T3) and thyroxine (T4). These hormones are essential for regulating metabolism growth and development. The activity of TPO is an important step in thyroid hormone production.

Biological function summary

TPO participates in the thyroid's hormone biosynthesis pathway. It forms a part of the multi-enzyme complex that includes thyroglobulin and iodide. The enzyme facilitates the iodination of tyrosine residues on thyroglobulin and the coupling of iodotyrosines important for creating active thyroid hormones. TPO's enzymatic function is important for maintaining appropriate levels of circulating T3 and T4 impacting various physiological processes in the body.

Pathways

TPO contributes to the thyroid hormone synthesis pathway which is a critical pathway for metabolic regulation. It works in close association with other proteins such as thyroglobulin and sodium/iodide symporter (NIS) to ensure efficient thyroid hormone production. TPO's involvement in these pathways indicates its importance in the endocrine system's normal function and homeostasis.

Associated diseases and disorders

TPO is often associated with autoimmune thyroid diseases like Hashimoto's thyroiditis and Graves' disease. High TPO antibodies such as anti-TPO antibodies are commonly detected in these conditions indicating an autoimmune response against the thyroid. The presence of high anti-thyroid peroxidase antibodies can lead to the destruction of thyroid tissue often resulting in hypothyroidism. TPO's connection to these diseases highlights its role in autoimmune conditions affecting thyroid function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383), expandable thumbnail

    Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)

    All lanes: Western blot - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383) at 1/1000 dilution

    All lanes: Human thyroid lysate at 10 µg

    Predicted band size: 103 kDa, 35 kDa, 54 kDa

  • Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383), expandable thumbnail

    Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)

    Thyroid Peroxidase/TPO was immunoprecipitated from 0.35 mg Human thyroid whole cell lysate 10 μg with 109383 at 1/100 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human thyroid whole cell lysate 10 μg

    Lane 2: ab109383 IP in Human thyroid whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109383 in Human thyroid whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)

    Predicted band size: 103 kDa

    Observed band size: 110 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)

    ab109383, at a 1/100 dilution, staining Thyroid Peroxidase/TPO in paraffin-embedded Human thyroid gland tissue

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Multiplex immunohistochemistry - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (ab109383)

    Fluorescence multiplex immunohistochemical analysis of Human thyroid gland tissue (Formalin/PFA-fixed paraffin-embedded sections).

    Panel A: merged staining of anti-Thyroglobulin (Anti-Thyroglobulin antibody [EPR3614(2)] ab151539, magenta; Opal™690), anti-Thyroid Peroxidase/TPO (ab109383, green; Opal™520) and anti-PAX8 (Anti-PAX8 antibody [SP348] - N-terminal ab227707, red; Opal™570) on human thyroid gland. Panel B: anti-Thyroid Peroxidase/TPO stained on cytoplasm of follicular epithelial cells. Panel C: anti-PAX8 stained on nucleus of follicular epithelial cells. Panel D: anti-Thyroglobulin stained on colloid. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

    The section was incubated in three rounds of staining: in the order of Anti-Thyroglobulin antibody [EPR3614(2)] ab151539 at 1/5000 dilution (0.167 μg/ml), ab109383 at 1/1500 dilution (0.072 μg/ml) , and Anti-PAX8 antibody [SP348] - N-terminal ab227707 at 1/200 dilution (5.22 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. DAPI (blue) was used as a nuclear counter stain.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

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