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Rabbit Recombinant Monoclonal Thyroxine Binding Globulin antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (AB280954), expandable thumbnail
  • Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (AB280954), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (AB280954), expandable thumbnail
  • Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (AB280954), expandable thumbnail
  • Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (AB280954), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)IHC-P
Human
Not recommended
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/300
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Major thyroid hormone transport protein in serum.

Alternative names

TBG, SERPINA7, Thyroxine-binding globulin, Serpin A7, T4-binding globulin

Recommended products

Rabbit Recombinant Monoclonal Thyroxine Binding Globulin antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24453-121
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Thyroxine Binding Globulin (TBG) also called globulin 23 is a glycoprotein that binds thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3). It facilitates the transport of these hormones through the bloodstream to various tissues. This binding globulin has a molecular weight of approximately 54 kDa. TBG is predominantly expressed in the liver and is an essential component within the circulatory system for hormone regulation. Although there is no substance called 'globulin green' TBG plays an important role in maintaining the systemic balance of thyroid hormones.

Biological function summary

The thyroxine-binding globulin helps maintain adequate levels of thyroid hormones in circulation ensuring a ready reserve supply to target tissues. TBG acts as a carrier protein and does not form part of a larger complex. It provides stability to the hormones and prevents their premature clearance from the bloodstream. Without it the availability and functionality of thyroid hormones within the body would be compromised affecting metabolic and growth functions.

Pathways

Thyroxine-binding globulin is critical in the thyroid hormone signaling pathway. This pathway regulates metabolism growth and development through the action of thyroid hormones. TBG is closely related to other hormone-binding proteins such as transthyretin and albumin which also participate in thyroid hormone transport albeit with different affinities. Its presence ensures that these hormones remain available for cellular uptake and action within various tissues influencing energy expenditure and developmental processes.

Associated diseases and disorders

The function and levels of thyroxine-binding globulin have significant implications in conditions like hypothyroidism and hyperthyroidism. Altered TBG levels either due to genetic variations or external factors can lead to abnormal blood concentrations of thyroid hormones affecting growth and metabolism. In such disorders the interaction between TBG and thyroid hormones becomes critical. TBG levels can be influenced by other proteins like sex hormone-binding globulin (SHBG) which may reflect changes in the hormone-binding protein landscape during disease states contributing to dysregulated hormonal balance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Thyroxine Binding Globulin with ab280954 at 1/300 (5.92 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the plasma in human colon. The section was incubated with ab280954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954), expandable thumbnail

    Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Thyroxine Binding Globulin was immunoprecipitated from 0.35 mg Human lung tissue lysate with ab280954 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280954 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human lung tissue lysate 10 ug

    Lane 2: ab280954 IP in Human lung tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab280954 in Human lung tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Predicted band size: 46 kDa

    Observed band size: 49 kDa, 54 kDa

  • Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling Thyroxine Binding Globulin with ab280954 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa cells.

  • Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954), expandable thumbnail

    Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.
    Samples are non-boiled as boiling may cause protein aggregates.
    The MW observed is consistent with the literature (PMID: 12107243).
    Thyroxine-binding globulin is highly expressed in plasma. (PMID:820495, 8106612).
    Bands of 54KDa and 49KDa respectively represent the full length and endogenous cleavage form.
    Exposure time: Lane 1: 15 seconds Lane 2: 37 seconds Lane 3, 4: 109 seconds.

    All lanes: Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954) at 1/1000 dilution

    Lane 1: Human plasma at 20 µL

    Lane 2: Human small intestine tissue lysate at 40 µg

    Lane 3: Human lung tissue lysate 40ug at 40 µg

    Lane 4: Human skeletal muscle lysate at 40 µg

    Secondary

    Lane 1: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Lanes 2 - 4: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 46 kDa

    Observed band size: 49 kDa, 54 kDa

  • Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954), expandable thumbnail

    Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Thyroxine Binding Globulin was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) supernatant with ab280954 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280954 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) supernatant 10 μl

    Lane 2: ab280954 IP in HepG2 supernatant

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab280954 in HepG2 supernatant

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Predicted band size: 46 kDa

    Observed band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Thyroxine Binding Globulin with ab280954 at 1/300 (5.92 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the plasma in human placenta. The section was incubated with ab280954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling Thyroxine Binding Globulin with ab280954 at 1/300 (5.92 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human skeletal muscle (PMID: 8143919). The section was incubated with ab280954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (ab280954)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells labelling Thyroxine Binding Globulin with ab280954 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HEK-293T cells.

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Product protocols

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