Rabbit Recombinant Monoclonal Thyroxine Binding Globulin antibody. Carrier free. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105284.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962)")
![Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunoprecipitation-img11728.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962)")
![Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--flow-cytometry-intracellular-img70648.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962)")
![Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--western-blot-img147469.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962)")
![Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunoprecipitation-img4511.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (AB280962)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Major thyroid hormone transport protein in serum.
Alternative names
TBG, TBG, SERPINA7, Thyroxine-binding globulin, Serpin A7, T4-binding globulin
Recommended products
Rabbit Recombinant Monoclonal Thyroxine Binding Globulin antibody. Carrier free. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR24453-121
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab280962 is the carrier-free version of Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thyroxine Binding Globulin (TBG) also called globulin 23 is a glycoprotein that binds thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3). It facilitates the transport of these hormones through the bloodstream to various tissues. This binding globulin has a molecular weight of approximately 54 kDa. TBG is predominantly expressed in the liver and is an essential component within the circulatory system for hormone regulation. Although there is no substance called 'globulin green' TBG plays an important role in maintaining the systemic balance of thyroid hormones.
- Biological function summary
The thyroxine-binding globulin helps maintain adequate levels of thyroid hormones in circulation ensuring a ready reserve supply to target tissues. TBG acts as a carrier protein and does not form part of a larger complex. It provides stability to the hormones and prevents their premature clearance from the bloodstream. Without it the availability and functionality of thyroid hormones within the body would be compromised affecting metabolic and growth functions.
- Pathways
Thyroxine-binding globulin is critical in the thyroid hormone signaling pathway. This pathway regulates metabolism growth and development through the action of thyroid hormones. TBG is closely related to other hormone-binding proteins such as transthyretin and albumin which also participate in thyroid hormone transport albeit with different affinities. Its presence ensures that these hormones remain available for cellular uptake and action within various tissues influencing energy expenditure and developmental processes.
- Associated diseases and disorders
The function and levels of thyroxine-binding globulin have significant implications in conditions like hypothyroidism and hyperthyroidism. Altered TBG levels either due to genetic variations or external factors can lead to abnormal blood concentrations of thyroid hormones affecting growth and metabolism. In such disorders the interaction between TBG and thyroid hormones becomes critical. TBG levels can be influenced by other proteins like sex hormone-binding globulin (SHBG) which may reflect changes in the hormone-binding protein landscape during disease states contributing to dysregulated hormonal balance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105284.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
This data was developed using Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Thyroxine Binding Globulin with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 at 1/300 (5.92 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the plasma in human colon. The section was incubated with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunoprecipitation-img11728.jpg "Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
This data was developed using Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954, the same antibody clone in a different buffer formulation.
Thyroxine Binding Globulin was immunoprecipitated from 0.35 mg Human lung tissue lysate with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human lung tissue lysate 10 ug
Lane 2: Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 IP in Human lung tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 in Human lung tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This blot was developed using a higher sensitivity ECL substrate.
All lanes: Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954)
Predicted band size: 46 kDa
Observed band size: 49 kDa, 54 kDa
![Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--flow-cytometry-intracellular-img70648.jpg "Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
This data was developed using Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling Thyroxine Binding Globulin with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HeLa cells.
![Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--western-blot-img147469.jpg "Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
This data was developed using 280954, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregates.
The MW observed is consistent with the literature (PMID: 12107243).
Thyroxine-binding globulin is highly expressed in plasma. (PMID:820495, 8106612).
Bands of 54KDa and 49KDa respectively represent the full length and endogenous cleavage form.
Exposure time: Lane 1: 15 seconds Lane 2: 37 seconds Lane 3, 4: 109 seconds.
All lanes: Western blot - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954) at 1/1000 dilution
Lane 1: Human plasma at 20 µL
Lane 2: Human small intestine tissue lysate at 40 µg
Lane 3: Human lung tissue lysate 40ug at 40 µg
Lane 4: Human skeletal muscle lysate at 40 µg
SecondaryLane 1: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 2 - 4: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 46 kDa
Observed band size: 49 kDa, 54 kDa
![Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunoprecipitation-img4511.jpg "Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
This data was developed using Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954, the same antibody clone in a different buffer formulation.
Thyroxine Binding Globulin was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) supernatant with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) supernatant 10 μl
Lane 2: Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 IP in HepG2 supernatant
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 in HepG2 supernatant
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] (Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954)
Predicted band size: 46 kDa
Observed band size: 54 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135822.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
This data was developed using Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling Thyroxine Binding Globulin with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 at 1/300 (5.92 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the plasma in human placenta. The section was incubated with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111823.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
This data was developed using Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling Thyroxine Binding Globulin with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 at 1/300 (5.92 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on human skeletal muscle (PMID: 8143919). The section was incubated with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--flow-cytometry-intracellular-img27184.jpg "Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)
This data was developed using Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells labelling Thyroxine Binding Globulin with Anti-Thyroxine Binding Globulin antibody [EPR24453-121] ab280954 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HEK-293T cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135822.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111823.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
![Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962), expandable thumbnail](https://content.abcam.com/products/images/thyroxine-binding-globulin-antibody-epr24453-121-bsa-and-azide-free-ab280962--flow-cytometry-intracellular-img27184.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Thyroxine Binding Globulin antibody [EPR24453-121] - BSA and Azide free (ab280962)")
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