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AB285114

Anti-Thyroxine Binding Globulin antibody [EPR24453-49] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Thyroxine Binding Globulin antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

TBG, SERPINA7, Thyroxine-binding globulin, Serpin A7, T4-binding globulin

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Sandwich ELISA - Anti-Thyroxine Binding Globulin antibody [EPR24453-49] - BSA and Azide free (Detector) (AB285114)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Thyroxine Binding Globulin antibody [EPR24453-49] - BSA and Azide free (Detector) (AB285114)

Example of Human Thyroxine Binding Globulin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24453-49

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab285114 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Thyroxine Binding Globulin. The recommended pair for sandwich ELISA is:
Capture: ab285008, Human Thyroxine Binding Globulin Capture Antibody (unconjugated)
Detector: ab285114, Human Thyroxine Binding Globulin Detector Antibody (unconjugated)
The reference range value is 19.5 - 2,500 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thyroxine Binding Globulin (TBG) also called globulin 23 is a glycoprotein that binds thyroid hormones primarily thyroxine (T4) and triiodothyronine (T3). It facilitates the transport of these hormones through the bloodstream to various tissues. This binding globulin has a molecular weight of approximately 54 kDa. TBG is predominantly expressed in the liver and is an essential component within the circulatory system for hormone regulation. Although there is no substance called 'globulin green' TBG plays an important role in maintaining the systemic balance of thyroid hormones.
Biological function summary

The thyroxine-binding globulin helps maintain adequate levels of thyroid hormones in circulation ensuring a ready reserve supply to target tissues. TBG acts as a carrier protein and does not form part of a larger complex. It provides stability to the hormones and prevents their premature clearance from the bloodstream. Without it the availability and functionality of thyroid hormones within the body would be compromised affecting metabolic and growth functions.

Pathways

Thyroxine-binding globulin is critical in the thyroid hormone signaling pathway. This pathway regulates metabolism growth and development through the action of thyroid hormones. TBG is closely related to other hormone-binding proteins such as transthyretin and albumin which also participate in thyroid hormone transport albeit with different affinities. Its presence ensures that these hormones remain available for cellular uptake and action within various tissues influencing energy expenditure and developmental processes.

The function and levels of thyroxine-binding globulin have significant implications in conditions like hypothyroidism and hyperthyroidism. Altered TBG levels either due to genetic variations or external factors can lead to abnormal blood concentrations of thyroid hormones affecting growth and metabolism. In such disorders the interaction between TBG and thyroid hormones becomes critical. TBG levels can be influenced by other proteins like sex hormone-binding globulin (SHBG) which may reflect changes in the hormone-binding protein landscape during disease states contributing to dysregulated hormonal balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major thyroid hormone transport protein in serum.
See full target information SERPINA7
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Product promise

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For full details, please see our Terms & Conditions

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