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Rabbit Recombinant Monoclonal Thyroxine (T4) antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples.

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Images

Competitive ELISA - Anti-Thyroxine (T4) antibody [EPR28885-68] - BSA and Azide free (AB322008), expandable thumbnail
  • Competitive ELISA - Anti-Thyroxine (T4) antibody [EPR28885-68] - BSA and Azide free (AB322008), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
cELISA
Chemical
Tested

Tested
Tested

Species
Chemical
Dilution info
-
Notes

-

Target data

Recommended products

Rabbit Recombinant Monoclonal Thyroxine (T4) antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28885-68
Purification technique
Affinity purification Protein A
Specificity

This antibody has 0.13% cross reaction with 3, 3', 5'-Triiodo-L-thyronine and 0.28% cross reaction with 3, 3', 5-Triiodo-L-thyronine, but does not cross-react with 3, 5-Diiodo-L-thyronine and 3, 3'-Diiodo-L-thyronine.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab322008 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for competitive ELISAs to quantify Thyroxine (T4).

The reference range value is 0.25-32 ng/mL.

Please note that the range provided for this antibody is only an estimation. Performance of the antibody depends on the specific characteristics of your assay. We guarantee the product works in competitive ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Thyroxine also known as T4 is a hormone that originates from the thyroid gland. It plays a mechanical role in regulating the basal metabolic rate by controlling the rate of oxygen use by cells. Its molecular weight is approximately 776.87 Da. T4 primarily circulates in the bloodstream bound to carrier proteins such as thyroxine-binding globulin transthyretin and albumin. The thyroid gland synthesizes and releases T4 in response to stimulation by thyroid-stimulating hormone (TSH). This hormone is important for maintaining metabolic processes and occurs both in human and mouse models demonstrating its cross-species significance.

Biological function summary

Thyroxine acts on tissues throughout the body affecting growth development and metabolism. It is not part of a simple structure but rather influences multiple aspects of endocrinological systems. On a cellular level T4 enters target cells and converts to its active form triiodothyronine (T3) which then binds to thyroid hormone receptors inside the nucleus. This binding alters gene transcription modulating metabolic activity within those cells. Although T4 itself does not form complexes its metabolic conversion and subsequent gene regulatory functions play complex roles in cellular processes.

Pathways

Thyroxine is integral to the hypothalamic-pituitary-thyroid (HPT) axis and metabolic pathway. The HPT axis involves TSH which stimulates the release of T4 forming a feedback loop essential for maintaining homeostasis. T4 impacts various metabolic pathways directly influencing proteins such as deiodinases which convert T4 into the more active T3. Additionally T4 affects pathways involving energy production thermoregulation and lipid metabolism showcasing its widespread importance in physiological processes.

Associated diseases and disorders

Thyroxine imbalance is directly linked to conditions like hypothyroidism and hyperthyroidism. In hypothyroidism there's insufficient T4 leading to symptoms such as fatigue weight gain and depression. Conversely hyperthyroidism involves excess T4 causing symptoms like weight loss increased heart rate and anxiety. Proteins such as TSH and thyroglobulin are significantly affected by these conditions as their levels can indicate thyroid gland dysfunction. Understanding T4's role provides insights into therapeutic interventions targeting thyroid-related disorders highlighting the importance of effective monitoring through assays such as TSH ELISA and other diagnostic tools.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Competitive ELISA - Anti-Thyroxine (T4) antibody [EPR28885-68] - BSA and Azide free (ab322008), expandable thumbnail

    Competitive ELISA - Anti-Thyroxine (T4) antibody [EPR28885-68] - BSA and Azide free (ab322008)

    0.2 ug/mL Thyroxine (T4)-crosslink-BSA was coated onto 96-wells. Serial dilution of free Thyroxine (T4) (0, 0.25, 0.5, 1, 2, 4, 8, 16, 32 ng/mL) was added (50 uL). This was followed by adding 0.1 ug/mL ab322008 (50 uL) into each well. The plate was incubated for 30 mins. After washing, 1:20000 diluted GAR Fc-HRP was added and incubated for 30 mins. After washing, 100 uL of TMB was added and incubated for 5-10 mins without shaking for color development. Then 100 uL of stop solution (4.9% Orthophosphoric acid) was dispensed and OD was read at 450nm on a microplate reader within 5-10 mins.

  • Competitive ELISA - Anti-Thyroxine (T4) antibody [EPR28885-68] - BSA and Azide free (ab322008), expandable thumbnail

    Competitive ELISA - Anti-Thyroxine (T4) antibody [EPR28885-68] - BSA and Azide free (ab322008)

    ab322008 (0.1 ug/mL) is specific to Thyroxine (T4) and the percent cross reactivity with other compounds is demonstrated in the table.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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