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Rabbit Recombinant Monoclonal TIA1 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (AB263953), expandable thumbnail
  • Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (AB263953), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (AB263953), expandable thumbnail
  • Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (AB263953), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (AB263953), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/4000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

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Target data

Function

RNA-binding protein involved in the regulation of alternative pre-RNA splicing and mRNA translation by binding to uridine-rich (U-rich) RNA sequences (PubMed:11106748, PubMed:12486009, PubMed:17488725, PubMed:8576255). Binds to U-rich sequences immediately downstream from a 5' splice sites in a uridine-rich small nuclear ribonucleoprotein (U snRNP)-dependent fashion, thereby modulating alternative pre-RNA splicing (PubMed:11106748, PubMed:8576255). Preferably binds to the U-rich IAS1 sequence in a U1 snRNP-dependent manner; this binding is optimal if a 5' splice site is adjacent to IAS1 (By similarity). Activates the use of heterologous 5' splice sites; the activation depends on the intron sequence downstream from the 5' splice site, with a preference for a downstream U-rich sequence (PubMed:11106748). By interacting with SNRPC/U1-C, promotes recruitment and binding of spliceosomal U1 snRNP to 5' splice sites followed by U-rich sequences, thereby facilitating atypical 5' splice site recognition by U1 snRNP (PubMed:11106748, PubMed:12486009, PubMed:17488725). Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIAR mRNA (By similarity). Acts as a modulator of alternative splicing for the apoptotic FAS receptor, thereby promoting apoptosis (PubMed:11106748, PubMed:17488725, PubMed:1934064). Binds to the 5' splice site region of FAS intron 5 to promote accumulation of transcripts that include exon 6 at the expense of transcripts in which exon 6 is skipped, thereby leading to the transcription of a membrane-bound apoptotic FAS receptor, which promotes apoptosis (PubMed:11106748, PubMed:17488725, PubMed:1934064). Binds to a conserved AU-rich cis element in COL2A1 intron 2 and modulates alternative splicing of COL2A1 exon 2 (PubMed:17580305). Also binds to the equivalent AT-rich element in COL2A1 genomic DNA, and may thereby be involved in the regulation of transcription (PubMed:17580305). Binds specifically to a polypyrimidine-rich controlling element (PCE) located between the weak 5' splice site and the intronic splicing silencer of CFTR mRNA to promote exon 9 inclusion, thereby antagonizing PTB1 and its role in exon skipping of CFTR exon 9 (PubMed:14966131). Involved in the repression of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs), including target ARE-bearing mRNAs encoding TNF and PTGS2 (By similarity). Also participates in the cellular response to environmental stress, by acting downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SGs), leading to stress-induced translational arrest (PubMed:10613902). Formation and recruitment to SGs is regulated by Zn(2+) (By similarity). Possesses nucleolytic activity against cytotoxic lymphocyte target cells (PubMed:1934064). Isoform Short. Displays enhanced splicing regulatory activity compared with TIA isoform Long.

Alternative names

Cytotoxic granule associated RNA binding protein TIA1, Nucleolysin TIA-1 isoform p40, RNA-binding protein TIA-1, T-cell-restricted intracellular antigen-1, p40-TIA-1, TIA-1, TIA1

Recommended products

Rabbit Recombinant Monoclonal TIA1 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22999-80
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab263953 is the carrier-free version of Anti-TIA1 antibody [EPR22999-80] ab263945.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TIA1 also known as T-cell intracellular antigen 1 is a protein that plays a role in RNA binding and regulation of gene expression. It possesses a molecular mass of approximately 43 kDa. TIA1 is characterized by its presence in the cytoplasm of various cell types including immune cells and neuronal cells. It contains three RNA recognition motifs (RRM) that enable it to interact with its RNA targets and modulate their fate during cellular stress.

Biological function summary

TIA1 functions in the assembly of stress granules which are aggregates of proteins and RNAs that form in response to cellular stress. It is part of a complex dynamic system that regulates mRNA translation by directing untranslated mRNAs to stress granules thereby influencing gene expression at the post-transcriptional level. TIA1 is implicated in apoptosis and can bind with other proteins like HuR to affect mRNA stability and translation.

Pathways

TIA1 participates in stress response signaling and apoptotic pathways. It is involved in the regulation of mRNA metabolism playing a critical role in the cellular stress response pathway by modulating the translation and stability of mRNAs during stress. TIA1 interacts with kinases like JNK to mediate these stress responses and works alongside related proteins including G3BP to organize mRNAs into stress granules.

Associated diseases and disorders

TIA1 has been linked to conditions such as neurodegenerative diseases and certain types of cancer. Mutations or dysregulations in TIA1 expression can lead to disorders characterized by defective stress granule formation and RNA metabolism. In the context of amyotrophic lateral sclerosis (ALS) TIA1 interacts with proteins like TDP-43 which have roles in similar pathological aggregation processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Western blot - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:30533021).

    Anti-TIA1 antibody [EPR22999-80] ab263945 was shown to specifically react with TIA1 in wild-type HAP1 cells as signal was lost in TIA1 knockout cells. Wild-type and TIA1 knockout samples were subjected to SDS-PAGE. Anti-TIA1 antibody [EPR22999-80] ab263945 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Exposure time: Lanes 1-3: 103 seconds Lane 4: 26 seconds Lanes 5-6: 103 seconds Lane 7: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR22999-80] ab263945).

    All lanes: Western blot - Anti-TIA1 antibody [EPR22999-80] (Anti-TIA1 antibody [EPR22999-80] ab263945) at 1/1000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: TIA1 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

    Lane 4: HuT-78 (human Sezary syndrome cutaneous T lymphocyte), whole cell lysate at 20 µg

    Lane 5: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate at 20 µg

    Lane 6: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

    Lane 7: Human tonsil tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa

    Observed band size: 42 kDa, 43 kDa

  • Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    TIA1 was immunoprecipitated from 0.35 mg HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate with Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate 10ug

    Lane 2: Anti-TIA1 antibody [EPR22999-80] ab263945 IP in HuT-78 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TIA1 antibody [EPR22999-80] ab263945 in HuT-78 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    Truncated form (~16kDa) of TIA1 was also immunoprecipitated. (PMID: 25224594).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR22999-80] ab263945).

    All lanes: Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] (Anti-TIA1 antibody [EPR22999-80] ab263945)

    Predicted band size: 43 kDa

    Observed band size: 16 kDa, 42 kDa, 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling TIA1 with Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/4000 dilution (0.15ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in tumor-infiltrating T-lymphocytes of human gastric cancer (PMID: 10658910) is observed.

    The section was incubated with Anti-C Reactive Protein antibody [EPR22862-10] ab256492 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR22999-80] ab263945).

  • Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    TIA1 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 10ug with Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 10ug

    Lane 2: Anti-TIA1 antibody [EPR22999-80] ab263945 IP in Jurkat whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TIA1 antibody [EPR22999-80] ab263945 in Jurkat whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Truncated form (~16kDa) of TIA1 was also immunoprecipitated. (PMID: 25224594).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR22999-80] ab263945).

    All lanes: Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] (Anti-TIA1 antibody [EPR22999-80] ab263945)

    Predicted band size: 43 kDa

    Observed band size: 16 kDa, 42 kDa, 43 kDa

  • Flow Cytometry (Intracellular) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized TIA1 knockout HAP1 (TIA1 knockout human chronic myelogenous leukemia near-haploid cell line, (Left) / WT HAP1 (Right) cells labelling TIA1 with Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR22999-80] ab263945).

  • Flow Cytometry (Intracellular) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Jurkat (human T cell leukemia T lymphocyte) cells labelling TIA1 with Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR22999-80] ab263945).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    Immunohistochemical analysis of paraffin-embedded Human classical hodgkin lymphoma tissue labeling TIA1 with Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/4000 dilution (0.15ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in T-lymphocytes of human classical hodgkin lymphoma (PMID: 19096012) is observed.

    The section was incubated with Anti-C Reactive Protein antibody [EPR22862-10] ab256492 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR22999-80] ab263945).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling TIA1 with Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/4000 dilution (0.15ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in T-lymphocytes of human spleen (PMID: 23510456) is observed.

    The section was incubated with Anti-C Reactive Protein antibody [EPR22862-10] ab256492 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR22999-80] ab263945).

  • Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    This data was developed using Anti-TIA1 antibody [EPR22999-80] ab263945, the same antibody clone in a different buffer formulation.

    Immunoprecipitation of TIA1 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 µg of 86050 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Immunoprecipitation - Anti-TIA1 antibody [EPR22999-80] (Anti-TIA1 antibody [EPR22999-80] ab263945) at 1 µg

    All lanes: HAP1 cells

  • Immunocytochemistry/ Immunofluorescence - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    This data was developed using Anti-TIA1 antibody [EPR22999-80] ab263945, the same antibody clone in a different buffer formulation.

    Anti-TIA1 antibody [EPR22999-80] ab263945 was shown to react with TIA1 in wild-type HAP1 cells in immunocytochemistry with loss of signal observed in a TIA1 knockout cell line. Anti-TIA1 antibody [EPR22999-80] ab263945 at 1/600 dilution.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

  • Western blot - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953), expandable thumbnail

    Western blot - Anti-TIA1 antibody [EPR22999-80] - BSA and Azide free (ab263953)

    This data was developed using Anti-TIA1 antibody [EPR22999-80] ab263945, the same antibody clone in a different buffer formulation.

    Anti-TIA1 antibody [EPR22999-80] ab263945 was shown to react with TIA1 in wild-type HAP1 cells in Western blot with loss of signal observed in a TIA1 knockout cell line. Wild-type HAP1 and TIA1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-TIA1 antibody [EPR22999-80] ab263945 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-TIA1 antibody [EPR22999-80] (Anti-TIA1 antibody [EPR22999-80] ab263945) at 1/1000 dilution

    Lane 1: Wild-type HAP1 lysate at 50 µg

    Lane 2: TIA1 knock-out HAP1 lysate at 50 µg

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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