Anti-TIA1 antibody [EPR9304]
- KO Validated
- RabMAb
- Recombinant
- 20ul selling size
- What is this?
2
(1 Review)
|
(20 Publications)
Anti-TIA1 antibody [EPR9304] (ab140595) is a rabbit monoclonal antibody detecting TIA1 in Western Blot, IP, IHC-P, ICC/IF. Suitable for Human, Mouse.
- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications
View Alternative Names
Cytotoxic granule associated RNA binding protein TIA1, Nucleolysin TIA-1 isoform p40, RNA-binding protein TIA-1, T-cell-restricted intracellular antigen-1, p40-TIA-1, TIA-1, TIA1
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-TIA1 antibody [EPR9304] (AB140595)
ab140595 staining TIA1 in wild-type Hap1 cells (top panel) and TIA1 knockout Hap1 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab140595 at 1/250 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] (AB140595)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TIA1 with unpurified ab140595 at a dilution of 1/100.
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Anti-TIA1 antibody [EPR9304] (AB140595)
Immunocytochemistry/Immunofluorescence analysis of HuT-78 cells labelling TIA1 with purified ab140595 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] (AB140595)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TIA1 with purified ab140595 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
- IP
Lab
Immunoprecipitation - Anti-TIA1 antibody [EPR9304] (AB140595)
ab140595 (purified) at 1/40 immunoprecipitating TIA1 in HuT-78 whole cell lysate.
Lane 1 (input) : HuT-78 whole cell lysate (10μg)
Lane 2 (+) : ab140595 + HuT-78 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab140595 in HuT-78 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.
All lanes:
Immunoprecipitation - Anti-TIA1 antibody [EPR9304] (ab140595)
Predicted band size: 43 kDa
Observed band size: 42 kDa,43 kDa
false
- IP
Lab
Immunoprecipitation - Anti-TIA1 antibody [EPR9304] (AB140595)
Immunoprecipitation of TIA1 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 µg of ab263945 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Immunoprecipitation - Anti-TIA1 antibody [EPR9304] (ab140595) at 1 µg
All lanes:
HAP1 cells
false
- WB
Lab
Western blot - Anti-TIA1 antibody [EPR9304] (AB140595)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-TIA1 antibody [EPR9304] (ab140595) at 1/5000 dilution
Lane 1:
Jurkat whole cell lysate at 20 µg
Lane 2:
K562 whole cell lysate at 20 µg
Lane 3:
HUT-78 whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 42-43 kDa
false
- WB
Lab
Western blot - Anti-TIA1 antibody [EPR9304] (AB140595)
Lane 1 : Wild-type HAP1 cell lysate (40 μg)
Lane 2 : TIA1 knockout HAP1 cell lysate (40 μg)
Lane 3 : Jurkat cell lysate (40 μg)
Lane 4 : K562 cell lysate (40 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab140595 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab140595 was shown to specifically react with TIA1 when TIA1 knockout samples were used. Wild-type and TIA1 knockout samples were subjected to SDS-PAGE. ab140595 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-TIA1 antibody [EPR9304] (ab140595)
Predicted band size: 43 kDa
false
- WB
Unknown
Western blot - Anti-TIA1 antibody [EPR9304] (AB140595)
All lanes:
Western blot - Anti-TIA1 antibody [EPR9304] (ab140595)
Lane 1:
HuT-78 cell lysate at 10 µg
Lane 2:
Jurkat cell lysate at 10 µg
Lane 3:
Molt-4 cell lysate at 10 µg
Lane 4:
K562 cell lysate at 10 µg
Secondary
All lanes:
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 43 kDa
false
- WB
Lab
Western blot - Anti-TIA1 antibody [EPR9304] (AB140595)
ab140595 was shown to react with TIA1 in wild-type HAP1 cells in Western blot with loss of signal observed in a TIA1 knockout cell line. Wild-type HAP1 and TIA1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab140595 overnight at 4 °C at a 1/5000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot - Anti-TIA1 antibody [EPR9304] (ab140595) at 1/5000 dilution
Lane 1:
Wild-type HAP1 lysate at 50 µg
Lane 2:
TIA1 knock-out HAP1 lysate at 50 µg
false
- WB
Lab
Western blot - Anti-TIA1 antibody [EPR9304] (AB140595)
Blocking and dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-TIA1 antibody [EPR9304] (ab140595) at 1/5000 dilution
All lanes:
NIH/3T3 whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 42-43 kDa
false
Related conjugates and formulations (7)
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-TIA1 antibody [EPR9304]
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Anti-TIA1 antibody [EPR9304] - BSA and Azide free
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-TIA1 antibody [EPR9304]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-TIA1 antibody [EPR9304]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-TIA1 antibody [EPR9304]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-TIA1 antibody [EPR9304]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-TIA1 antibody [EPR9304]
Reactivity data
Product details
What is this antibody validated in?
Anti-TIA1 antibody [EPR9304] (ab140595) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of TIA1?
Anti-TIA1 [EPR9304] (ab140595) specifically detects a band for TIA1 (UniProt: P31483) at a molecular weight of 43kDa.
Trusted by the scientific community
Anti-TIA1 [EPR9304] (ab140595) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Specificity confirmed
The specificity of Anti-TIA1 antibody [EPR9304] (ab140595) has been confirmed by Western blot testing in TIA1 Knockout HAP1 cells.
Other related products
We have a range of other formats of antibody clone [EPR9304] also available for your convenience: ab140595, Alexa Fluor® 488 - ab196382, Carrier free - ab230829, Alexa Fluor® 647 - ab311116, Alexa Fluor® 594 - ab311742, Alexa Fluor® 568 - ab313022, Alexa Fluor® 555 - ab313224, Alexa Fluor® 750 - ab321117
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TIA1 functions in the assembly of stress granules which are aggregates of proteins and RNAs that form in response to cellular stress. It is part of a complex dynamic system that regulates mRNA translation by directing untranslated mRNAs to stress granules thereby influencing gene expression at the post-transcriptional level. TIA1 is implicated in apoptosis and can bind with other proteins like HuR to affect mRNA stability and translation.
Pathways
TIA1 participates in stress response signaling and apoptotic pathways. It is involved in the regulation of mRNA metabolism playing a critical role in the cellular stress response pathway by modulating the translation and stability of mRNAs during stress. TIA1 interacts with kinases like JNK to mediate these stress responses and works alongside related proteins including G3BP to organize mRNAs into stress granules.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (20)
Recent publications for all applications. Explore the full list and refine your search
European journal of immunology 55:e51885 PubMed40538191
2025
Applications
Unspecified application
Species
Unspecified reactive species
Translational neurodegeneration 14:22 PubMed40355949
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature immunology 26:557-566 PubMed40050432
2025
Applications
Unspecified application
Species
Unspecified reactive species
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409086 PubMed39804990
2025
Applications
Unspecified application
Species
Unspecified reactive species
F1000Research 12:745 PubMed38638178
2024
Applications
WB, IP
Species
Human, Human
Nucleic acids research 52:5356-5375 PubMed38366783
2024
Applications
Unspecified application
Species
Unspecified reactive species
European journal of haematology 109:513-518 PubMed35871391
2022
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 13:2306 PubMed35484142
2022
Applications
Unspecified application
Species
Unspecified reactive species
FEBS letters 595:2872-2896 PubMed34780058
2021
Applications
Unspecified application
Species
Unspecified reactive species
International journal of molecular sciences 22: PubMed34681673
2021
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com