Rabbit Recombinant Monoclonal TIA1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58526.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829)")
![Immunocytochemistry/ Immunofluorescence - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunocytochemistry-immunofluorescence-img22038.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829)")
![Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--western-blot-img11659.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829)")
![Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunoprecipitation-img21353.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65589.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (AB230829)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
7 products for Alternative Version
Target data
Function
Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
Alternative names
Nucleolysin TIA-1 isoform p40, RNA-binding protein TIA-1, T-cell-restricted intracellular antigen-1, p40-TIA-1, TIA-1, TIA1
Recommended products
Rabbit Recombinant Monoclonal TIA1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples.
Alternative names
Nucleolysin TIA-1 isoform p40, RNA-binding protein TIA-1, T-cell-restricted intracellular antigen-1, p40-TIA-1, TIA-1, TIA1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR9304
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab230829 is the carrier-free version of Anti-TIA1 antibody [EPR9304] ab140595.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
TIA1 also known as T-cell intracellular antigen 1 is a protein that plays a role in RNA binding and regulation of gene expression. It possesses a molecular mass of approximately 43 kDa. TIA1 is characterized by its presence in the cytoplasm of various cell types including immune cells and neuronal cells. It contains three RNA recognition motifs (RRM) that enable it to interact with its RNA targets and modulate their fate during cellular stress.
- Biological function summary
TIA1 functions in the assembly of stress granules which are aggregates of proteins and RNAs that form in response to cellular stress. It is part of a complex dynamic system that regulates mRNA translation by directing untranslated mRNAs to stress granules thereby influencing gene expression at the post-transcriptional level. TIA1 is implicated in apoptosis and can bind with other proteins like HuR to affect mRNA stability and translation.
- Pathways
TIA1 participates in stress response signaling and apoptotic pathways. It is involved in the regulation of mRNA metabolism playing a critical role in the cellular stress response pathway by modulating the translation and stability of mRNAs during stress. TIA1 interacts with kinases like JNK to mediate these stress responses and works alongside related proteins including G3BP to organize mRNAs into stress granules.
- Associated diseases and disorders
TIA1 has been linked to conditions such as neurodegenerative diseases and certain types of cancer. Mutations or dysregulations in TIA1 expression can lead to disorders characterized by defective stress granule formation and RNA metabolism. In the context of amyotrophic lateral sclerosis (ALS) TIA1 interacts with proteins like TDP-43 which have roles in similar pathological aggregation processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58526.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)
This IHC data was generated using the same anti-TIA1 antibody clone, EPR9304, in a different buffer formulation (cat# Anti-TIA1 antibody [EPR9304] ab140595).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TIA1 with purified Anti-TIA1 antibody [EPR9304] ab140595 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunocytochemistry-immunofluorescence-img22038.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)")
Immunocytochemistry/ Immunofluorescence - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)
Immunocytochemistry/Immunofluorescence analysis of HuT-78 cells labelling TIA1 with purified Anti-TIA1 antibody [EPR9304] ab140595 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR9304] ab140595).
![Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--western-blot-img11659.jpg "Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)")
Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)
This WB data was generated using the same anti-TIA1 antibody clone, EPR9304, in a different buffer formulation (cat# Anti-TIA1 antibody [EPR9304] ab140595).
Lane 1: Wild-type HAP1 cell lysate (40 μg)
Lane 2: TIA1 knockout HAP1 cell lysate (40 μg)
Lane 3: Jurkat cell lysate (40 μg)
Lane 4: K562 cell lysate (40 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-TIA1 antibody [EPR9304] ab140595 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.Anti-TIA1 antibody [EPR9304] ab140595 was shown to specifically react with TIA1 when TIA1 knockout samples were used. Wild-type and TIA1 knockout samples were subjected to SDS-PAGE. Anti-TIA1 antibody [EPR9304] ab140595 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TIA1 antibody [EPR9304] (Anti-TIA1 antibody [EPR9304] ab140595)
Predicted band size: 43 kDa
![Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunoprecipitation-img21353.jpg "Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)")
Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)
Anti-TIA1 antibody [EPR9304] ab140595 (purified) at 1/40 immunoprecipitating TIA1 in HuT-78 whole cell lysate.
Lane 1 (input): HuT-78 whole cell lysate (10µg)
Lane 2 (+): Anti-TIA1 antibody [EPR9304] ab140595 + HuT-78 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TIA1 antibody [EPR9304] ab140595 in HuT-78 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR9304] ab140595).
All lanes: Immunoprecipitation - Anti-TIA1 antibody [EPR9304] (Anti-TIA1 antibody [EPR9304] ab140595)
Predicted band size: 43 kDa
Observed band size: 42 kDa, 43 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65589.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TIA1 with unpurified Anti-TIA1 antibody [EPR9304] ab140595 at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TIA1 antibody [EPR9304] ab140595).
![Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--western-blot-img438513.jpg "Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)")
Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)
This data was developed using Anti-TIA1 antibody [EPR9304] ab140595, the same antibody clone in a different buffer formulation.
Anti-TIA1 antibody [EPR9304] ab140595 was shown to react with TIA1 in wild-type HAP1 cells in Western blot with loss of signal observed in a TIA1 knockout cell line. Wild-type HAP1 and TIA1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-TIA1 antibody [EPR9304] ab140595 overnight at 4 °C at a 1/5000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Western blot - Anti-TIA1 antibody [EPR9304] (Anti-TIA1 antibody [EPR9304] ab140595) at 1/5000 dilution
Lane 1: Wild-type HAP1 lysate at 50 µg
Lane 2: TIA1 knock-out HAP1 lysate at 50 µg
![Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunoprecipitation-img438569.jpg "Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)")
Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)
This data was developed using Anti-TIA1 antibody [EPR9304] ab140595, the same antibody clone in a different buffer formulation.
Immunoprecipitation of TIA1 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 µg of Anti-TIA1 antibody [EPR22999-80] ab263945 pre-coupled to Protein A beads. Samples were then washed and processed for western blot.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Immunoprecipitation - Anti-TIA1 antibody [EPR9304] (Anti-TIA1 antibody [EPR9304] ab140595) at 1 µg
All lanes: HAP1 cells
![Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--western-blot-img438513.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)")
![Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829), expandable thumbnail](https://content.abcam.com/products/images/tia1-antibody-epr9304-bsa-and-azide-free-ab230829--immunoprecipitation-img438569.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TIA1 antibody [EPR9304] - BSA and Azide free (ab230829)")
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