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AB249497

Anti-TIAL1 antibody [EPR11323(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TIAL1 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Nucleolysin TIAR, TIA-1-related protein, TIAL1

1 Images
Western blot - Anti-TIAL1 antibody [EPR11323(B)] - BSA and Azide free (AB249497)
  • WB

Lab

Western blot - Anti-TIAL1 antibody [EPR11323(B)] - BSA and Azide free (AB249497)

This data was developed using the same antibody clone in a different buffer formulation (ab169547).

Lanes 1-4 : Merged signal (red and green). Green - ab169547 observed at 49 kDa. Red - loading control ab8245 observed at 36 kDa.

ab169547 Anti-TIAL1 antibody [EPR11323(B)] was shown to specifically react with TIAL1 in wild-type HeLa cells. Loss of signal was observed when knockout sample ab257743 was used. Wild-type and TIAL1 knockout samples were subjected to SDS-PAGE. ab169547 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TIAL1 antibody [EPR11323(B)] (<a href='/en-us/products/primary-antibodies/tial1-antibody-epr11323b-ab169547'>ab169547</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TIAL1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TIAL1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-tial1-knockout-hela-cell-line-ab265793'>ab265793</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

K-562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11323(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249497 is the carrier-free version of ab169547.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIAL1 also known as TIA-1 related protein is a 42 kDa protein that plays a mechanical role in RNA processing. It belongs to the RNA-binding protein family and contains three RNA recognition motifs and a glutamine-rich domain. TIAL1 is widely expressed in various tissues including the spleen thymus and brain. It influences the regulation of mRNA stability and translation specifically recognizing and binding to U-rich elements in the 3’-untranslated regions of target mRNAs.
Biological function summary

TIAL1 influences gene expression by associating with stress granules cytoplasmic aggregates formed in response to cellular stress. This association helps inhibit translation initiation modulating the cellular stress response. TIAL1 is part of a complex with other RNA-binding proteins that integrate signaling events and manage mRNA turnover and stress granule dynamics. Through this interaction it affects apoptosis and contributes to cell survival under stress conditions.

Pathways

Studies demonstrate that TIAL1 plays a role in the apoptotic signaling pathway and the stress response pathway. By interacting with proteins such as eIF2α kinase TIAL1 helps mediate the cellular response to environmental and oxidative stress. These pathways maintain cellular homeostasis and TIAL1 assists in regulating translation during stress ensuring proper cell function.

TIAL1 has relevance in neurodegenerative diseases like Alzheimer's and amyotrophic lateral sclerosis (ALS). The formation of stress granules influenced by TIAL1 is connected to the pathological misfolding of proteins implicated by related proteins such as TDP-43 and FUS. Faulty regulation of these processes may lead to the progression of neurodegenerative conditions highlighting TIAL1's significant role in their development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation (PubMed : 10613902, PubMed : 1326761, PubMed : 17488725, PubMed : 8576255). Shows a preference for uridine-rich RNAs (PubMed : 8576255). Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA (By similarity). Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1 (PubMed : 17488725). Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG) (PubMed : 10613902). Possesses nucleolytic activity against cytotoxic lymphocyte target cells (PubMed : 1326761). May be involved in apoptosis (PubMed : 1326761).
See full target information Nucleolysin TIAR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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