Rabbit Polyclonal TIAM1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TIAM1 aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Histology and histopathology 36:355-3652021MiR-590-5p regulates cell proliferation, apoptosis, migration and invasion in oral squamous cell carcinoma by targeting RECK.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei-Wei Bao,You-Ling Shi,Yan Ma,Xing-Hui Qu,Guang-Ming Pang,Lei YangPubMed 33447989
- Biochemical genetics :953-9652020TIAM1 Upregulation Confers NVP-BEZ235 Resistance to Breast Cancer Cells Through FGFR/STAT3 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Qiao,Man Li,Na Li,Lingzhi Xu,Gena Huang,Jing YuPubMed 32671511
- Archives of biochemistry and biophysics 663:101-1082018NF45 promotes esophageal squamous carcinoma cell invasion by increasing Rac1 activity through 14-3-3ε protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Wen-Jian et. al.PubMed 30550728
- Cellular physiology and biochemistry : internation 51:1763-17772018miR-10b Downregulated by DNA Methylation Acts as a Tumor Suppressor in HPV-Positive Cervical Cancer via Targeting Tiam1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiaomei Yu et. al.PubMed 30504727
- Oncogene 38:699-7152018MicroRNA 483-3p targets Pard3 to potentiate TGF-β1-induced cell migration, invasion, and epithelial-mesenchymal transition in anaplastic thyroid cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoping Zhang,Lin Liu,Xianzhao Deng,Dan Li,Haidong Cai,Yushui Ma,Chengyou Jia,Bo Wu,Youben Fan,Zhongwei LvPubMed 30171257
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TIAM1 aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13009
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Target data
Function
Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration.
Alternative names
Rho guanine nucleotide exchange factor TIAM1, T-lymphoma invasion and metastasis-inducing protein 1, TIAM-1, TIAM1
Recommended products
Rabbit Polyclonal TIAM1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TIAM1 aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TIAM1 aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13009
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIAM1 also known as T-cell lymphoma invasion and metastasis 1 is a significant regulator in cellular dynamics. It functions as a guanine nucleotide exchange factor (GEF) predominantly for the Rho family of GTPases particularly Rac1. With a mass of approximately 188 kDa TIAM1 is ubiquitously expressed in various tissues indicating its broad role in cellular activities. The protein modulates cytoskeletal organization and cell-migration by exchanging GDP for GTP on Rac1 therefore activating it.
- Biological function summary
TIAM1 influences several cellular processes such as cell polarity migration and growth. It forms a complex with several other proteins and plays a role in reorganizing the actin cytoskeleton. This ability to modulate the cytoskeleton impacts the cell's ability to move and change shape which are essential during developmental processes and in maintaining tissue architecture. TIAM1 also affects cell-cell adhesion through interactions with E-cadherin which suggests its involvement in stabilizing cellular contacts.
- Pathways
The role of TIAM1 can be observed prominently in the Wnt and PI3K/Akt signaling pathways. In the Wnt pathway TIAM1 interacts with the β-catenin pathway influencing processes like gene expression cell behavior and differentiation. It co-localizes with Dsh and APC influencing the signaling cascade that controls cell fate decisions. In the PI3K/Akt pathway it impacts cell survival and metabolism showing TIAM1's engagement with multiple proteins like PI3K and Akt which further affects cell proliferation and survival mechanisms.
- Associated diseases and disorders
TIAM1 shows a significant connection to cancer and neurological disorders. Its role in promoting cancer cell invasion and metastasis is evident in various tumor types where altered TIAM1 expression correlates with tumor progression and poor prognosis. In cancer it engages with proteins such as MMPs facilitating extracellular matrix degradation and metastasis. Furthermore aberrant TIAM1 expression or dysfunction is associated with some neurological disorders indicating its possible involvement in neurodevelopmental or neurodegenerative conditions where related proteins like Rac1 play an important part.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM1 antibody (ab211518)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling TIAM1 with ab211518 at 1/100 dilution.
Western blot - Anti-TIAM1 antibody (ab211518)
All lanes: Western blot - Anti-TIAM1 antibody (ab211518) at 1/1000 dilution
All lanes: ZR-75-1 cell line lysates at 35 µg
Predicted band size: 178 kDa
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Product protocols
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