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Rabbit Polyclonal TIAM1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TIAM1 aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human TIAM1 aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13009
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Target data

Function

Guanyl-nucleotide exchange factor that activates RHO-like proteins and connects extracellular signals to cytoskeletal activities. Activates RAC1, CDC42, and to a lesser extent RHOA and their downstream signaling to regulate processes like cell adhesion and cell migration.

Alternative names

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Rabbit Polyclonal TIAM1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TIAM1 aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human TIAM1 aa 1450-1550 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q13009
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

TIAM1 influences several cellular processes such as cell polarity migration and growth. It forms a complex with several other proteins and plays a role in reorganizing the actin cytoskeleton. This ability to modulate the cytoskeleton impacts the cell's ability to move and change shape which are essential during developmental processes and in maintaining tissue architecture. TIAM1 also affects cell-cell adhesion through interactions with E-cadherin which suggests its involvement in stabilizing cellular contacts.

Activity summary

TIAM1 also known as T-cell lymphoma invasion and metastasis 1 is a significant regulator in cellular dynamics. It functions as a guanine nucleotide exchange factor (GEF) predominantly for the Rho family of GTPases particularly Rac1. With a mass of approximately 188 kDa TIAM1 is ubiquitously expressed in various tissues indicating its broad role in cellular activities. The protein modulates cytoskeletal organization and cell-migration by exchanging GDP for GTP on Rac1 therefore activating it.

Pathways

The role of TIAM1 can be observed prominently in the Wnt and PI3K/Akt signaling pathways. In the Wnt pathway TIAM1 interacts with the β-catenin pathway influencing processes like gene expression cell behavior and differentiation. It co-localizes with Dsh and APC influencing the signaling cascade that controls cell fate decisions. In the PI3K/Akt pathway it impacts cell survival and metabolism showing TIAM1's engagement with multiple proteins like PI3K and Akt which further affects cell proliferation and survival mechanisms.

Associated diseases and disorders

TIAM1 shows a significant connection to cancer and neurological disorders. Its role in promoting cancer cell invasion and metastasis is evident in various tumor types where altered TIAM1 expression correlates with tumor progression and poor prognosis. In cancer it engages with proteins such as MMPs facilitating extracellular matrix degradation and metastasis. Furthermore aberrant TIAM1 expression or dysfunction is associated with some neurological disorders indicating its possible involvement in neurodevelopmental or neurodegenerative conditions where related proteins like Rac1 play an important part.

Product promise

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM1 antibody (ab211518), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM1 antibody (ab211518)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling TIAM1 with ab211518 at 1/100 dilution.

  • Western blot - Anti-TIAM1 antibody (ab211518), expandable thumbnail

    Western blot - Anti-TIAM1 antibody (ab211518)

    All lanes: Western blot - Anti-TIAM1 antibody (AB211518) at 1/1000 dilution

    All lanes: ZR-75-1 cell line lysates at 35 µg

    Predicted band size: 178 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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