AB199426

Anti-TIAM2 antibody [EPR16838]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal TIAM2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

KIAA2016, STEF, TIAM2, Rho guanine nucleotide exchange factor TIAM2, SIF and TIAM1-like exchange factor, T-lymphoma invasion and metastasis-inducing protein 2, TIAM-2

7 Images

Flow Cytometry (Intracellular) - Anti-TIAM2 antibody [EPR16838] (AB199426)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TIAM2 antibody [EPR16838] (AB199426)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow)cells labeling U TIAM2 with ab199426 at 1/450 dilution (red). The secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is Rabbit monoclonal IgG (black) and the cell without incubation with primary antibody and secondary antibody is blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM2 antibody [EPR16838] (AB199426)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM2 antibody [EPR16838] (AB199426)

Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling TIAM2 with ab199426 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human gliocytoma tissue is observed. Counter stained with Hematoxylin.
Negative control : Used only secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TIAM2 antibody [EPR16838] (AB199426)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TIAM2 antibody [EPR16838] (AB199426)

Immunocytochemistry/Immunofluorescence analysis of Raw264.7 cells labelling TIAM2 with ab199426 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control : PBS only.
Nuclear counter stain : DAPI.

Supplier Data

Western blot - Anti-TIAM2 antibody [EPR16838] (AB199426)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/10000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Lane 2:

293 (Human embryonic kidney) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 190 kDa

Observed band size: 193 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-TIAM2 antibody [EPR16838] (AB199426)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426) at 2000 Cells

All lanes:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 190 kDa

Observed band size: 193 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-TIAM2 antibody [EPR16838] (AB199426)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 mg/mL

Lane 4:

NIH/3T3 (mouse embryo fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 190 kDa

Observed band size: 193 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-TIAM2 antibody [EPR16838] (AB199426)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TIAM2 antibody [EPR16838] (ab199426) at 1/1000 dilution

All lanes:

C6 (rat glial tumor cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 190 kDa

Observed band size: 193 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16838

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/450", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIAM2 also known as T-cell lymphoma invasion and metastasis 2 is a guanine nucleotide exchange factor (GEF) with a mass of approximately 180 kDa. It plays a significant role mechanically by activating the small GTPase Rac1 leading to changes in actin cytoskeleton organization. This protein localizes in the cytoplasm and shows expression in many tissues but finds higher levels in the brain and lymphoid organs. Its role extends to cellular processes like cell migration proliferation and adhesion.

Biological function summary

TIAM2 contributes to intracellular signaling that governs cell shape and movement. It participates as part of a larger signaling complex that includes other GEFs and scaffold proteins. By facilitating the switch of Rac1 from inactive GDP-bound state to active GTP-bound state TIAM2 influences pathways that control the dynamics of the actin cytoskeleton important for cellular structural change highlighting its importance in the development of cellular architecture and motility.

Pathways

TIAM2 activates signaling cascades related to cell migration and differentiation. It plays an essential role in the Rac1-PAK pathway which regulates the actin cytoskeleton dynamics. This pathway interconnects with other signaling molecules like CDC42 and PAK kinases creating a network that controls cellular responses and contacts with the extracellular matrix. In this network TIAM2 acts as a facilitator for cellular adaptation to environmental stimuli.

TIAM2 links to conditions such as cancer metastasis and neurological disorders. Alterations in its expression or function contribute to the progression of certain cancers by promoting increased motility and invasive capabilities of tumor cells. Additionally abnormalities in TIAM2 activity have been observed in diseases like schizophrenia where it interacts with proteins such as DISC1 affecting neuronal signaling and stability. Understanding TIAM2's involvement in these diseases provides insight into therapeutic targets for managing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA.

See full target information TIAM2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 49:188-200 PubMed39533770

2024

SSX2IP promotes cell proliferation and migration in breast cancer by regulating FANCI.

Applications

Unspecified application

Species

Unspecified reactive species

Xianfu Liu,Xiaojing Zhang,Yansong Chen,Jingwei Tang,Hao Zhang,Gongsheng Jin

eNeuro 11: PubMed38871458

2024

RhoGEF Tiam2 Regulates Glutamatergic Synaptic Transmission in Hippocampal CA1 Pyramidal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Sadhna Rao,Feng Liang,Bruce E Herring

Journal of bone oncology 37:100461 PubMed36419799

2022

TIAM2 promotes proliferation and invasion of osteosarcoma cells by activating the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohua Cheng,Xinghan Huang,Weichun Guo

International journal of molecular sciences 23: PubMed36142328

2022

Contributes to Osimertinib Resistance, Cell Motility, and Tumor-Associated Macrophage M2-like Polarization in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Liang,Hua He,Shiyao Jiang,Yueying Liu,Jingjing Huang,Xiaoyan Sun,Yi Li,Yiqun Jiang,Li Cong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIAM2 antibody [EPR16838]

Flow Cytometry (Intracellular) - Anti-TIAM2 antibody [EPR16838] (AB199426)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIAM2 antibody [EPR16838] (AB199426)

Immunocytochemistry/ Immunofluorescence - Anti-TIAM2 antibody [EPR16838] (AB199426)

Western blot - Anti-TIAM2 antibody [EPR16838] (AB199426)

Western blot - Anti-TIAM2 antibody [EPR16838] (AB199426)

Western blot - Anti-TIAM2 antibody [EPR16838] (AB199426)

Western blot - Anti-TIAM2 antibody [EPR16838] (AB199426)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise