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AB17221

Anti-TICAM2 antibody

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(4 Publications)

Rabbit Polyclonal TICAM2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TICAM2.

View Alternative Names

TIRAP3, TIRP, TRAM, TICAM2, TIR domain-containing adapter molecule 2, TICAM-2, Putative NF-kappa-B-activating protein 502, TRIF-related adapter molecule, Toll-like receptor adaptor protein 3, Toll/interleukin-1 receptor domain-containing protein, MyD88-4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TICAM2 antibody (AB17221)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TICAM2 antibody (AB17221)

ab17221 at 10μg/ml staining TICAM2 in Raji cells by ICC/IF

Western blot - Anti-TICAM2 antibody (AB17221)
  • WB

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Western blot - Anti-TICAM2 antibody (AB17221)

Western Blot of PC-3 cell lysate labeling TICAM2 with Anti-TICAM2 antibody (ab17221) at 1μg/ml.

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Western blot - Anti-TICAM2 antibody (ab17221)

Predicted band size: 27 kDa

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Western blot - Anti-TICAM2 antibody (AB17221)
  • WB

Unknown

Western blot - Anti-TICAM2 antibody (AB17221)

Western blot using ab17221 at 1 μg/ml on PC-3 cell lysate.

All lanes:

Western blot - Anti-TICAM2 antibody (ab17221) at 1 µg/mL

All lanes:

PC-3 cell lysate

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TICAM2. The exact immunogen used to generate this antibody is proprietary information.

Q86XR7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TICAM2 also known as TRAM (TRIF-related adaptor molecule) plays an important role in the innate immune response by mediating signaling from Toll-like receptors (TLRs). It has a mass of approximately 27 kDa. TICAM2 is mainly expressed in immune cells such as macrophages and dendritic cells. It engages in complex interactions with TLRs specifically TLR4 to trigger downstream signaling cascades.
Biological function summary

The molecule participates in the modulation of immune responses. TICAM2 is a part of the MyD88-independent pathway interacting closely with TRIF (TIR-domain-containing adapter-inducing interferon-β) in signaling complexes. This interaction leads to the activation of transcription factors that stimulate the production of type I interferons and other cytokines critical for fighting viral infections.

Pathways

TICAM2 functions in the TLR signaling pathway bridging TLR4 and TRIF to regulate immune responses. It plays a significant role in the TRAM-TRIF pathway. This pathway is essential for activating NF-κB and IRF3 which lead to the expression of inflammatory genes. Proteins like TLR4 and TRIF are closely related to TICAM2 in these signaling pathways and contribute to immune regulation.

TICAM2 is associated with immune system-related conditions such as sepsis and autoimmune diseases. Its role in modulating inflammatory responses links it to these disorders. The interaction with TLR4 is significant as it implicates TICAM2 in the hyperactivation of inflammatory signaling pathways leading to excessive immune responses in sepsis. Similarly an overactive TICAM2-related pathway can contribute to autoimmune diseases by sustaining chronic inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a sorting adapter in different signaling pathways to facilitate downstream signaling leading to type I interferon induction (PubMed : 16603631, PubMed : 16757566, PubMed : 25385819, PubMed : 25825441). In TLR4 signaling, physically bridges TLR4 and TICAM1 and functionally transmits signal to TICAM1 in early endosomes after endocytosis of TLR4. In TLR2 signaling, physically bridges TLR2 and MYD88 and is required for the TLR2-dependent movement of MYD88 to endosomes following ligand engagement (PubMed : 25385819). Involved in IL-18 signaling and is proposed to function as a sorting adapter for MYD88 in IL-18 signaling during adaptive immune response (PubMed : 22685567). Forms a complex with RAB11FIP2 that is recruited to the phagosomes to promote the activation of the actin-regulatory GTPases RAC1 and CDC42 and subsequent phagocytosis of Gram-negative bacteria (PubMed : 30883606).. Isoform 2. Proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1 thereby disrupting the association of isoform 1 with TICAM1. May be involved in TLR4 degradation in late endosomes.
See full target information TICAM2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of oral science 17:31 PubMed40246812

2025

Lysine-specific demethylase 1 controls key OSCC preneoplasia inducer STAT3 through CDK7 phosphorylation during oncogenic progression and immunosuppression.

Applications

Unspecified application

Species

Unspecified reactive species

Amit Kumar Chakraborty,Rajnikant Dilip Raut,Kisa Iqbal,Chumki Choudhury,Thabet Alhousami,Sami Chogle,Alexa S Acosta,Lana Fagman,Kelly Deabold,Marilia Takada,Bikash Sahay,Vikas Kumar,Manish V Bais

EMBO reports 25:770-795 PubMed38182816

2024

DDX5 inhibits inflammation by modulating m6A levels of TLR2/4 transcripts during bacterial infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xu,Li-Yuan Liu,Fei-Jie Zhi,Yin-Juan Song,Zi-Hui Zhang,Bin Li,Fu-Ying Zheng,Peng-Cheng Gao,Su-Zi Zhang,Yu-Yu Zhang,Ying Zhang,Ying Qiu,Bo Jiang,Yong-Qing Li,Chen Peng,Yue-Feng Chu

Oncotarget 8:73372-73386 PubMed29088714

2017

Inhibition of LSD1 epigenetically attenuates oral cancer growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Saqer F Alsaqer,Mustafa M Tashkandi,Vinay K Kartha,Ya-Ting Yang,Yazeed Alkheriji,Andrew Salama,Xaralabos Varelas,Maria Kukuruzinska,Stefano Monti,Manish V Bais

PloS one 9:e98517 PubMed24870145

2014

Differences in PGE2 production between primary human monocytes and differentiated macrophages: role of IL-1β and TRIF/IRF3.

Applications

WB

Species

Human

Yukinori Endo,Ksenia Blinova,Tatiana Romantseva,Hana Golding,Marina Zaitseva
View all publications
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