AB181024

Anti-TID1 antibody [EPR12414]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TID1 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HCA57, TID1, DNAJA3, DnaJ protein Tid-1, Hepatocellular carcinoma-associated antigen 57, Tumorous imaginal discs protein Tid56 homolog, hTid-1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TID1 antibody [EPR12414] (AB181024)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TID1 antibody [EPR12414] (AB181024)

Immunofluorescent analysis of acetone fixed HeLa cells labeling TID1 using ab181024 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.

Supplier Data

Immunoprecipitation - Anti-TID1 antibody [EPR12414] (AB181024)

Lysate from Jurkat cells (Lane 1) and negative control (Lane 2) were immunoprecipitated with ab181024 at a 1/30 dilution. A specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TID1 antibody [EPR12414] (ab181024)

Predicted band size: 52 kDa

false

Supplier Data

Western blot - Anti-TID1 antibody [EPR12414] (AB181024)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-TID1 antibody [EPR12414] (ab181024) at 1/5000 dilution

Lane 1:

JAR cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 52 kDa

false

Carrier free

Anti-TID1 antibody [EPR12414] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12414

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TID1 also known as Tumorous Imaginal Disc 1 or Tid1 functions as a mitochondrial DnaJ homolog characterized by its cochaperone activity. TID1 assists in protein folding and degradation processes within the cell by interacting with heat shock proteins such as Hsp70. The protein has a molecular mass of approximately 43 kDa. TID1 is mainly expressed in the mitochondria of various tissues including the heart skeletal muscles and brain.

Biological function summary

TID1 participates in maintaining mitochondrial integrity and cellular homeostasis. It is part of a complex involving multiple proteins that mediate signal transduction and apoptosis regulation. TID1 supports the correct folding and assembly of proteins imported into mitochondria directly impacting mitochondrial function. Additionally TID1 interacts with key molecules involved in apoptotic pathways influencing cell survival outcomes.

Pathways

TID1 plays roles in apoptosis and mitochondrial import pathways. Within the apoptosis pathway TID1 interacts with proteins like Bax and Bcl-2 influencing the release of cytochrome c. This release triggers the apoptosome formation activating the caspase cascade leading to programmed cell death. In mitochondrial import pathways TID1 aids the entry and proper folding of nuclear-encoded proteins into the mitochondrial matrix highlighting its connection to these essential cellular processes.

Dysfunction in TID1 relates to cancer and neurodegenerative diseases. Mutations or altered expressions of TID1 can disrupt apoptosis leading to the unchecked cell proliferation seen in cancers. In neurodegenerative contexts TID1's role in mitochondrial function links it to conditions like Huntington's disease where mitochondrial dysfunction is evident. In these disorders TID1’s interaction with apoptosis-related proteins like Bax becomes important in understanding disease progression and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.

See full target information DNAJA3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The international journal of biochemistry & cell b 65:268-74 PubMed26117454

2015

Exposure to arginine analog canavanine induces aberrant mitochondrial translation products, mitoribosome stalling, and instability of the mitochondrial proteome.

Applications

Species

Human

Svetlana Konovalova,Taru Hilander,Fabricio Loayza-Puch,Koos Rooijers,Reuven Agami,Henna Tyynismaa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TID1 antibody [EPR12414]

Immunocytochemistry/ Immunofluorescence - Anti-TID1 antibody [EPR12414] (AB181024)

Immunoprecipitation - Anti-TID1 antibody [EPR12414] (AB181024)

Western blot - Anti-TID1 antibody [EPR12414] (AB181024)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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