Anti-TID1 antibody [EPR12414] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TID1 antibody [EPR12414] - BSA and Azide free (AB250291)

This data was developed using ab181024, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone fixed HeLa cells labeling TID1 using ab181024 at a 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor®555) was used as the secondary at a 1/200 dilution. Counterstain DAPI.

• IP

Supplier Data

Immunoprecipitation - Anti-TID1 antibody [EPR12414] - BSA and Azide free (AB250291)

This data was developed using ab181024, the same antibody clone in a different buffer formulation.

Lysate from Jurkat cells (Lane 1) and negative control (Lane 2) were immunoprecipitated with ab181024 at a 1/30 dilution. A specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TID1 antibody [EPR12414] (<a href='/en-us/products/primary-antibodies/tid1-antibody-epr12414-ab181024'>ab181024</a>)

Predicted band size: 52 kDa

false

• WB

Supplier Data

Western blot - Anti-TID1 antibody [EPR12414] - BSA and Azide free (AB250291)

This data was developed using ab181024, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TID1 antibody [EPR12414] (<a href='/en-us/products/primary-antibodies/tid1-antibody-epr12414-ab181024'>ab181024</a>) at 1/5000 dilution

Lane 1:

JAR cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 52 kDa

false