AB27851

Anti-TIE1 antibody [8C9]

Name: Anti-TIE1 antibody [8C9] (ab27851) | Abcam
Brand: Abcam Limited
SKU: AB27851
Price: 535 USD
Availability: InStock

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(6 Publications)

Mouse Monoclonal TIE1 antibody. Carrier free. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TIE1.

View Alternative Names

TIE, TIE1, Tyrosine-protein kinase receptor Tie-1

2 Images

Flow Cytometry - Anti-TIE1 antibody [8C9] (AB27851)

Flow Cyt

Unknown

Flow Cytometry - Anti-TIE1 antibody [8C9] (AB27851)

Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Supplier Data

Western blot - Anti-TIE1 antibody [8C9] (AB27851)

All lanes:

Western blot - Anti-TIE1 antibody [8C9] (ab27851)

Lane 1:

Recombinant human soluble TIE1

Lane 2:

Recombinant human soluble TIE2

Lane 3:

Recombinant mouse soluble TIE1

Lane 4:

Recombinant mouse soluble TIE2

Predicted band size: 125 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8C9

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human TIE1. The exact immunogen used to generate this antibody is proprietary information.

P35590

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "200000-500000 µg/mL", "WB-species-notes": "" }, "Recombinant fragment": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "200000-500000 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

This antibody was purified from cell culture supernatant by chromatography.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIE1 also known as Tyrosine Kinase with Immunoglobulin-like and EGF-like Domains 1 plays a role as a receptor tyrosine kinase. It has a molecular mass of approximately 130 kDa. TIE1 is mainly expressed in vascular endothelial cells and is involved in the vascular system. It shares structural characteristics with other tyrosine kinases providing a framework for signaling and interaction. TIE1 activation involves phosphorylation leading to subsequent signaling cascades in endothelial cells.

Biological function summary

TIE1 associates with angiogenesis and blood vessel integrity. It forms a complex with other proteins notably TIE2 within the endothelial cells where it modulates endothelial cell survival and proliferation. Its role becomes particularly significant in dynamic environments where new blood vessel formation or remodeling occurs. TIE1 interacts with angiopoietins providing a network essential for maintaining vascular homeostasis and integrity.

Pathways

TIE1 interacts within angiopoietin-TIE signaling pathways. This pathway plays a critical role in vascular maturation and stability. TIE1 working in conjunction with TIE2 and the angiopoietins orchestrates the intricate balance between vessel stabilization and sprouting needed for healthy vascular function. These interactions link TIE1 with core processes in endothelial cell communication and vascular development.

TIE1 shows association with cancer and atherosclerosis. Its interaction with TIE2 and angiopoietin proteins contributes to pathological angiogenesis often observed in tumor growth and progression. In atherosclerosis TIE1 influences endothelial dysfunction and vascular inflammation. These diseases highlight TIE1's contribution to pathological processes highlighting the importance of its precise regulation in maintaining vascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.

See full target information TIE1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiovascular and thoracic research 13:131-140 PubMed34326967

2021

Resveratrol reduced the detrimental effects of malondialdehyde on human endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mehdi Hassanpour,Çıgır Biray Avci,Reza Rahbarghazi,Aysa Rezabakhsh,Alireza Nourazarian,Elahe Nabat,Farzaneh Fathi,Majid Khaksar

Annals of translational medicine 9:232 PubMed33708859

2021

MiR-4729 regulates TIE1 mRNA m6A modification and angiogenesis in hemorrhoids by targeting METTL14.

Applications

Unspecified application

Species

Unspecified reactive species

Te Liu,Haikun Zhou,Hong Lu,Chunsheng Luo,Qingming Wang,Yunhua Peng,Wei Yang,Yaojie Xin

Frontiers in cell and developmental biology 9:629274 PubMed33644068

2021

Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Jiajun Tang,Jie Liu,Bo Yan,Bin Yan,Minjun Huang,Zhongmin Zhang,Liang Wang

Stem cell research & therapy 11:139 PubMed32216836

2020

Autophagy modulation altered differentiation capacity of CD146 cells toward endothelial cells, pericytes, and cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Mehdi Hassanpour,Jafar Rezaie,Masoud Darabi,Amirataollah Hiradfar,Reza Rahbarghazi,Mohammad Nouri

Journal of cellular and molecular medicine 23:2595-2609 PubMed30672120

2019

Neurotrophin-3 acts on the endothelial-mesenchymal transition of heterotopic ossification in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Liang Wang,He Cao,Nan Chen,Bin Yan,Xiang Ao,Huiyu Zhao,Jun Chu,Minjun Huang,Zhongmin Zhang

Nature communications 9:994 PubMed29520052

2018

Expansion of functional personalized cells with specific transgene combinations.

Applications

Unspecified application

Species

Unspecified reactive species

Christoph Lipps,Franziska Klein,Tom Wahlicht,Virginia Seiffert,Milada Butueva,Jeannette Zauers,Theresa Truschel,Martin Luckner,Mario Köster,Roderick MacLeod,Jörn Pezoldt,Jochen Hühn,Qinggong Yuan,Peter Paul Müller,Henning Kempf,Robert Zweigerdt,Oliver Dittrich-Breiholz,Thomas Pufe,Rainer Beckmann,Wolf Drescher,Jose Riancho,Carolina Sañudo,Thomas Korff,Bertram Opalka,Vera Rebmann,Joachim R Göthert,Paula M Alves,Michael Ott,Roland Schucht,Hansjörg Hauser,Dagmar Wirth,Tobias May

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIE1 antibody [8C9]

Flow Cytometry - Anti-TIE1 antibody [8C9] (AB27851)

Western blot - Anti-TIE1 antibody [8C9] (AB27851)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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