Rabbit Polyclonal TIE1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TIE1.
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- Cancer discovery 12:484-5012021Mesenchymal Lineage Heterogeneity Underlies Nonredundant Functions of Pancreatic Cancer-Associated Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErin J Helms,Mark W Berry,R Crystal Chaw,Christopher C DuFort,Duanchen Sun,M Kathrina Onate,Chet Oon,Sohinee Bhattacharyya,Hannah Sanford-Crane,Wesley Horton,Jennifer M Finan,Ariana Sattler,Rosemary Makar,David W Dawson,Zheng Xia,Sunil R Hingorani,Mara H ShermanPubMed 34548310
- Cancers 12:2020Potential of Tyrosine Kinase Receptor TIE-1 as Novel Therapeutic Target in High-PI3K-Expressing Ovarian Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuewei Zhang et. al.PubMed 32604863
- Theranostics 10:7245-72592020Dual inhibition of PFKFB3 and VEGF normalizes tumor vasculature, reduces lactate production, and improves chemotherapy in glioblastoma: insights from protein expression profiling and MRI.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunfeng Zhang et. al.PubMed 32641990
- Journal of translational medicine 15:2032017Metastatic pathway and the microvascular and physicochemical microenvironments of human melanoma xenografts.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TIE1. The exact immunogen used to generate this antibody is proprietary information. Database link P35590
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Alternative antigen retrieval method: Tris-EDTA buffer pH 8.0 |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
Alternative names
TIE, TIE1, Tyrosine-protein kinase receptor Tie-1
Recommended products
Rabbit Polyclonal TIE1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TIE1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TIE1. The exact immunogen used to generate this antibody is proprietary information. Database link P35590
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIE1 also known as Tyrosine Kinase with Immunoglobulin-like and EGF-like Domains 1 plays a role as a receptor tyrosine kinase. It has a molecular mass of approximately 130 kDa. TIE1 is mainly expressed in vascular endothelial cells and is involved in the vascular system. It shares structural characteristics with other tyrosine kinases providing a framework for signaling and interaction. TIE1 activation involves phosphorylation leading to subsequent signaling cascades in endothelial cells.
- Biological function summary
TIE1 associates with angiogenesis and blood vessel integrity. It forms a complex with other proteins notably TIE2 within the endothelial cells where it modulates endothelial cell survival and proliferation. Its role becomes particularly significant in dynamic environments where new blood vessel formation or remodeling occurs. TIE1 interacts with angiopoietins providing a network essential for maintaining vascular homeostasis and integrity.
- Pathways
TIE1 interacts within angiopoietin-TIE signaling pathways. This pathway plays a critical role in vascular maturation and stability. TIE1 working in conjunction with TIE2 and the angiopoietins orchestrates the intricate balance between vessel stabilization and sprouting needed for healthy vascular function. These interactions link TIE1 with core processes in endothelial cell communication and vascular development.
- Associated diseases and disorders
TIE1 shows association with cancer and atherosclerosis. Its interaction with TIE2 and angiopoietin proteins contributes to pathological angiogenesis often observed in tumor growth and progression. In atherosclerosis TIE1 influences endothelial dysfunction and vascular inflammation. These diseases highlight TIE1's contribution to pathological processes highlighting the importance of its precise regulation in maintaining vascular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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3 product images
Western blot - Anti-TIE1 antibody (ab111547)
7.5% SDS PAGE
All lanes: Western blot - Anti-TIE1 antibody (ab111547) at 1/1000 dilution
All lanes: A549 lysate at 30 µg/mL
Predicted band size: 125 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody (ab111547)
Immunohistochemical analysis of paraffin embedded Colon carcinoma, using ab111547 at 1:500 dilution.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-TIE1 antibody (ab111547)
TIE1 western blot using anti-TIE1 antibody ab111547. Publication image and figure legend from Zhang, J., Xue, W., et al., 2020, Theranostics, PubMed 32641990.
ab111547 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab111547 please see the product overview.
Molecular profiling of angiogenesis-associated proteins after dual therapy and the effect of dual therapy on Tie1 expression. (A) Heatmap and the fold change of the differently expressed proteins (n = 9). (B) Western blot of Tie1 in ECs and tumor cells after treated with BEV or BEV+3PO for 24 h (n = 6). The top 12 relevant pathways based on DEPs from dual therapy group (C) and BEV monotherapy group (D). All data are means ± SD. *P < 0.05 compared with control.
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