AB111547

Anti-TIE1 antibody

Name: Anti-TIE1 antibody (ab111547) | Abcam
Brand: Abcam Limited
SKU: AB111547

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(5 Publications)

Rabbit Polyclonal TIE1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TIE1.

View Alternative Names

TIE, TIE1, Tyrosine-protein kinase receptor Tie-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody (AB111547)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody (AB111547)

Immunohistochemical analysis of paraffin embedded Colon carcinoma, using ab111547 at 1 : 500 dilution.

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Western blot - Anti-TIE1 antibody (AB111547)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TIE1 antibody (ab111547) at 1/1000 dilution

All lanes:

A549 lysate at 30 µg/mL

Predicted band size: 125 kDa

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CiteAb

Western blot - Anti-TIE1 antibody (AB111547)

TIE1 western blot using anti-TIE1 antibody ab111547. Publication image and figure legend from Zhang, J., Xue, W., et al., 2020, Theranostics, PubMed 32641990.

ab111547 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab111547 please see the product overview.

Molecular profiling of angiogenesis-associated proteins after dual therapy and the effect of dual therapy on Tie1 expression. (A) Heatmap and the fold change of the differently expressed proteins (n = 9). (B) Western blot of Tie1 in ECs and tumor cells after treated with BEV or BEV+3PO for 24 h (n = 6). The top 12 relevant pathways based on DEPs from dual therapy group (C) and BEV monotherapy group (D). All data are means ± SD. *p < 0.05 compared with control.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TIE1. The exact immunogen used to generate this antibody is proprietary information.

P35590

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Alternative antigen retrieval method: Tris-EDTA buffer pH 8.0</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIE1 also known as Tyrosine Kinase with Immunoglobulin-like and EGF-like Domains 1 plays a role as a receptor tyrosine kinase. It has a molecular mass of approximately 130 kDa. TIE1 is mainly expressed in vascular endothelial cells and is involved in the vascular system. It shares structural characteristics with other tyrosine kinases providing a framework for signaling and interaction. TIE1 activation involves phosphorylation leading to subsequent signaling cascades in endothelial cells.

Biological function summary

TIE1 associates with angiogenesis and blood vessel integrity. It forms a complex with other proteins notably TIE2 within the endothelial cells where it modulates endothelial cell survival and proliferation. Its role becomes particularly significant in dynamic environments where new blood vessel formation or remodeling occurs. TIE1 interacts with angiopoietins providing a network essential for maintaining vascular homeostasis and integrity.

Pathways

TIE1 interacts within angiopoietin-TIE signaling pathways. This pathway plays a critical role in vascular maturation and stability. TIE1 working in conjunction with TIE2 and the angiopoietins orchestrates the intricate balance between vessel stabilization and sprouting needed for healthy vascular function. These interactions link TIE1 with core processes in endothelial cell communication and vascular development.

TIE1 shows association with cancer and atherosclerosis. Its interaction with TIE2 and angiopoietin proteins contributes to pathological angiogenesis often observed in tumor growth and progression. In atherosclerosis TIE1 influences endothelial dysfunction and vascular inflammation. These diseases highlight TIE1's contribution to pathological processes highlighting the importance of its precise regulation in maintaining vascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.

See full target information TIE1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 28:378 PubMed38939621

2024

Gene expression profiling and the isocitrate dehydrogenase mutational landscape of temozolomide‑resistant glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wu-Fu Chen,Jimmy Ming-Jung Chuang,San-Nan Yang,Nan-Fu Chen,Manojit Bhattacharya,Hsin-Tzu Liu,Kuldeep Dhama,Chiranjib Chakraborty,Zhi-Hong Wen

Cancer discovery 12:484-501 PubMed34548310

2021

Mesenchymal Lineage Heterogeneity Underlies Nonredundant Functions of Pancreatic Cancer-Associated Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Erin J Helms,Mark W Berry,R Crystal Chaw,Christopher C DuFort,Duanchen Sun,M Kathrina Onate,Chet Oon,Sohinee Bhattacharyya,Hannah Sanford-Crane,Wesley Horton,Jennifer M Finan,Ariana Sattler,Rosemary Makar,David W Dawson,Zheng Xia,Sunil R Hingorani,Mara H Sherman

Cancers 12: PubMed32604863

2020

Potential of Tyrosine Kinase Receptor TIE-1 as Novel Therapeutic Target in High-PI3K-Expressing Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuewei Zhang,Masumi Ishibashi,Kazuyuki Kitatani,Shogo Shigeta,Hideki Tokunaga,Masafumi Toyoshima,Muneaki Shimada,Nobuo Yaegashi

Theranostics 10:7245-7259 PubMed32641990

2020

Dual inhibition of PFKFB3 and VEGF normalizes tumor vasculature, reduces lactate production, and improves chemotherapy in glioblastoma: insights from protein expression profiling and MRI.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Zhang,Wei Xue,Kai Xu,Liang Yi,Yu Guo,Tian Xie,Haipeng Tong,Bo Zhou,Shunan Wang,Qing Li,Heng Liu,Xiao Chen,Jingqin Fang,Weiguo Zhang

Journal of translational medicine 15:203 PubMed29017512

2017

Metastatic pathway and the microvascular and physicochemical microenvironments of human melanoma xenografts.

Applications

IHC

Species

Mouse

Ruixia Huang,Lise Mari K Andersen,Einar K Rofstad

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIE1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIE1 antibody (AB111547)

Western blot - Anti-TIE1 antibody (AB111547)

Western blot - Anti-TIE1 antibody (AB111547)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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