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AB151704

Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)]

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(7 Publications)

Rabbit Recombinant Monoclonal TIE1 phospho Y1007 antibody. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 7 publications.

View Alternative Names

TIE, TIE1, Tyrosine-protein kinase receptor Tie-1

4 Images
Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (AB151704)
  • WB

Lab

Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (AB151704)

Blocking buffer : 5% BSA/TBST
Dilution buffer : 5% BSA /TBST for primary antibody, 5% NFDM/TBST for secondary antibody

All lanes:

Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

Lane 1:

HUVEC cell lysate treated with pervanadate at 10 µg

Lane 2:

HUVEC cell lysate treated with pervanadate at 10 µg with TIE2 (phospho Y992) peptide

Lane 3:

HUVEC cell lysate treated with pervanadate at 10 µg with TIE2 unmodified peptide

Lane 4:

HUVEC cell lysate treated with pervanadate at 10 µg with TIE1 (phospho Y1007) peptide

Lane 5:

HUVEC cell lysate treated with pervanadate at 10 µg with TIE1 unmodified peptide

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000000 dilution

Observed band size: 125 kDa

false

Exposure time: 5s

Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (AB151704)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (AB151704)

Immunocytochemistry/Immunofluorescence analysis of HUVEC () cells labelling TIE2 + TIE1 (phospho Y992 + Y1007) with ab151704 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) at a dilution of 1/500 and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000. Nuclei were counterstained with DAPI (blue).

Confocal image showing increased cytoplasmic staining after PER (Pervanadate, 1mM, 30min) treatment on HUVEC cells. The LP treatment decreased the PER induced cytoplasmic staining.

Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (AB151704)
  • WB

Lab

Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (AB151704)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

Lane 1:

Untreated HUVEC whole cell lysates at 10 µg

Lane 2:

HUVEC treated with Pervanadate whole cell lysates at 10 µg

Lane 3:

HUVEC treated with Pervanadate whole cell lysates, then the membrane was incubated with phosphatase. at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 125 kDa

false

Exposure time: 10s

Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (AB151704)
  • Dot

Supplier Data

Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (AB151704)

Dot blot analysis of TIE2 (pY992) phospho peptide (lane 1) and TIE2 non-phospho peptide (lane 2) labelling TIE2 (phospho Y992) with ab151704 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

  • Carrier free

    Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1053(N)(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab151704 only detects TIE1 phosphorylated at tyrosine 1007 and TIE2 phosphorylated at tyrosine 992.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIE1 and TIE2 also known as Tyrosine Kinase with Immunoglobulin and EGF homology domains 1 and 2 are receptor tyrosine kinases. TIE1 has a molecular mass of roughly 130 kDa while TIE2 is about 145 kDa. These receptors express mainly in endothelial cells and they localize on the cell surface. Their structure allows them to bind specific ligands leading to signal transduction that influences cell behaviors.
Biological function summary

These receptors play significant roles in vascular development and maintenance. TIE1 and TIE2 form a complex with angiopoietins a group of growth factors important for blood vessel maturation and stability. The interactions between TIE receptors and angiopoietins control processes like endothelial cell survival migration and permeability making them essential for normal and pathological angiogenesis.

Pathways

TIE1 and TIE2 receptors feature prominently in the angiogenesis pathway and the endothelial cell signaling pathway. They interact closely with the angiopoietin family and influence downstream signaling through pathways involving proteins like PI3K/Akt and ERK/MAPK. These pathways are important for regulating endothelial cell functions linking TIE receptors to the maintenance of vascular integrity and response to environmental cues.

Disruptions in TIE1 and TIE2 signaling associate with conditions like cancer and cardiovascular disease. Aberrant TIE2 signaling correlates with tumor angiogenesis and metastasis often involving interactions with VEGF another key angiogenic factor. The dysfunction of these receptors may also contribute to atherosclerosis where improper endothelial cell communication leads to vascular inflammation and plaque formation. Understanding these connections aids in developing targeted therapies for managing such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis.
See full target information TIE1 phospho Y1007
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:70 PubMed39940043

2025

Pericyte-derived extracellular vesicles improve vascular barrier function in sepsis via the Angpt1/PI3K/AKT pathway and pericyte recruitment: an in vivo and in vitro study.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Sen Zhang,Ao Yang,Xi Luo,He-Nan Zhou,Yi-Yan Liu,Dai-Qin Bao,Jie Zhang,Jia-Tao Zang,Qing-Hui Li,Tao Li,Liang-Ming Liu

Frontiers in cell and developmental biology 11:1178045 PubMed37274734

2023

Role of UDP-glucose ceramide glucosyltransferase in venous malformation.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Chen,Yuan Wang,Liangliang Kong,Yi Ji,Jie Cui,Weimin Shen

International journal of molecular sciences 23: PubMed35806141

2022

Glycation of Tie-2 Inhibits Angiopoietin-1 Signaling Activation and Angiopoietin-1-Induced Angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Zhou,Tangting Chen,Yongjie Li,Jingcan You,Xin Deng,Ni Chen,Tian Li,Youkun Zheng,Rong Li,Mao Luo,Jianbo Wu,Liqun Wang

Communications biology 4:1370 PubMed34876695

2021

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xihua Yue,Neeraj Tiwari,Lianhui Zhu,Hai Dang Truong Ngo,Jae-Min Lim,Bopil Gim,Shuaiyang Jing,Yijing Wang,Yi Qian,Intaek Lee

Frontiers in cardiovascular medicine 8:707897 PubMed34651022

2021

Notch Intracellular Domain Plasmid Delivery via Poly(Lactic-Co-Glycolic Acid) Nanoparticles to Upregulate Notch Pathway Molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria L Messerschmidt,Uday Chintapula,Aneetta E Kuriakose,Samantha Laboy,Thuy Thi Dang Truong,LeNaiya A Kydd,Justyn Jaworski,Zui Pan,Hashem Sadek,Kytai T Nguyen,Juhyun Lee

Scientific reports 11:14021 PubMed34234265

2021

An engineered tetra-valent antibody fully activates the Tie2 receptor with comparable potency to its natural ligand angiopoietin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yukari Koya,Hiromi Nara,Shigenori Yagi,Chihoko Ueno,Masazumi Kamohara

Journal of cellular biochemistry 120:7115-7124 PubMed30378162

2018

Improved angiogenic activity of endothelial progenitor cell in diabetic patients treated with insulin plus metformin.

Applications

Unspecified application

Species

Unspecified reactive species

Simin Asadian,Mahdi Alibabrdel,Nazanin Daei,Hadi Cheraghi,Seyedeh Maedeh Jafari,Elnaz Noshadirad,Masoome Jabarpour,Vahid Siavashi,Seyed Mahdi Nassiri
View all publications
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