Rabbit Recombinant Monoclonal TIE2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.
Images
![Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (AB307557), expandable thumbnail](https://content.abcam.com/products/images/tie2-antibody-epr26540-78-bsa-and-azide-free-ab307557--western-blot-img405725.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (AB307557)")
![Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (AB307557), expandable thumbnail](https://content.abcam.com/products/images/tie2-antibody-epr26540-78-bsa-and-azide-free-ab307557--western-blot-img405724.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (AB307557)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1.
Alternative names
Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, hTIE2, TEK, TIE2, VMCM, VMCM1
Recommended products
Rabbit Recombinant Monoclonal TIE2 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples.
Alternative names
Angiopoietin-1 receptor, Endothelial tyrosine kinase, Tunica interna endothelial cell kinase, Tyrosine kinase with Ig and EGF homology domains-2, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK, hTIE2, TEK, TIE2, VMCM, VMCM1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR26540-78
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
TIE2 also known as TEK or CD202B is a receptor tyrosine kinase with a mass of approximately 145 kDa. It plays an important role in vascular development and maintenance. TIE2 is mainly expressed in endothelial cells which line the interior surface of blood vessels. It binds angiopoietins predominantly Angiopoietin-1 (Angpt1) influencing vascular stability and permeability. Researchers use TIE2 antibodies including anti-TIE and anti-clone to study its role and functions across various contexts.
- Biological function summary
TIE2 plays a central role in angiogenesis and blood vessel maturation. It forms a receptor complex with other components to mediate its effects. This interaction mainly involves Angpt1 which binds to TIE2 stabilizing blood vessels and preserving their integrity. TIE2 also participates in cell survival and migration processes necessary for normal vascular function. Researchers often perform Angpt1 ELISA in bulk to quantify interactions involving TIE2 in experiments.
- Pathways
TIE2 is important in the angiopoietin-TIE signaling pathway regulating vascular development. TIE2 is related to proteins such as Angpt1 which are centrally involved in this pathway. It also interacts with various signaling molecules including phosphatidylinositol-3-kinase (PI3K) which contributes to cell survival pathways. Alterations in the TIE2 signaling cascade can lead to aberrant angiogenesis and related pathologies.
- Associated diseases and disorders
TIE2 is implicated in several vascular diseases and cancer. Mutations or dysregulation of TIE2 can result in venous malformations and further compromise blood vessel integrity. In cancer TIE2 expression can influence tumor angiogenesis enhancing tumor growth and metastasis. TIE2 interacts with proteins like vascular endothelial growth factor (VEGF) which are involved in neovascularization and cancer progression. Understanding these interactions helps target therapeutic strategies in relevant diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (ab307557), expandable thumbnail](https://content.abcam.com/products/images/tie2-antibody-epr26540-78-bsa-and-azide-free-ab307557--western-blot-img405725.jpg "Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (ab307557)")
Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (ab307557)
This data was developed using 307556, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: TIE 2 is specifically expressed in endothelial cells and their progenitors (PMID:?8386827; PMID:?1630810; PMID:?8382358).
Low expression: mouse thymus (PMID:?8395828).
Lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
The identity of the bands lower than 75 kDa is unknown.
Exposure time:All lanes: Western blot - Anti-TIE2 antibody [EPR26540-78] (Anti-TIE2 antibody [EPR26540-78] ab307556) at 1/1000 dilution
Lane 1: Mouse lu tissue fresh lysate 20 μg
Lane 2: Mouse thymus tissue fresh lysate 20 μg
Lane 3: bEnd.3 (mouse brain endothelial cell) whole cell fresh lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 150 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTTIE 2 is specifically expressed in endothelial cells and their progenitors (PMID:?8386827; PMID:?1630810; PMID:?8382358).
Low expression: mouse thymus (PMID:?8395828).
Lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
The identity of the bands lower than 75 kDa is unknown.
Exposure time:![Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (ab307557), expandable thumbnail](https://content.abcam.com/products/images/tie2-antibody-epr26540-78-bsa-and-azide-free-ab307557--western-blot-img405724.jpg "Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (ab307557)")
Western blot - Anti-TIE2 antibody [EPR26540-78] - BSA and Azide free (ab307557)
This data was developed using 307556, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: TIE 2 is specifically expressed in endothelial cells and their progenitors (PMID: 8386827; PMID: 1630810; PMID: 8382358).
Negative control: HEK-293 (PMID: 17189382; PMID: 15851516).
The identity of the bands at approximately 60 kDa and 37 kDa is unknown.
This blot was developed using a high sensitivity ECL substrate.
180 seconds
Exposure time:All lanes: Western blot - Anti-TIE2 antibody [EPR26540-78] (Anti-TIE2 antibody [EPR26540-78] ab307556) at 1/1000 dilution
Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 20 μg
Lane 2: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 150 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBSTTIE 2 is specifically expressed in endothelial cells and their progenitors (PMID: 8386827; PMID: 1630810; PMID: 8382358).
Negative control: HEK-293 (PMID: 17189382; PMID: 15851516).
The identity of the bands at approximately 60 kDa and 37 kDa is unknown.
This blot was developed using a high sensitivity ECL substrate.
Exposure time: 180 seconds
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