Rabbit Recombinant Monoclonal TRIM24 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TRIM24 aa 1000 to C-terminus.
Images
![Western blot - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (AB314090), expandable thumbnail](https://content.abcam.com/products/images/tif1-alpha-trim24-antibody-blr225k-bsa-free-ab314090--western-blot-img420592.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (AB314090)")
![Immunoprecipitation - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (AB314090), expandable thumbnail](https://content.abcam.com/products/images/tif1-alpha-trim24-antibody-blr225k-bsa-free-ab314090--immunoprecipitation-img420593.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (AB314090)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human TRIM24 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15164
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation. After sufficient DNA repair has occurred, TP53 activates TRIM24 transcription, ultimately leading to TRIM24-mediated TP53 ubiquitination and degradation (PubMed:24820418). Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity). Participates also in innate immunity by mediating the specific 'Lys-63'-linked ubiquitination of TRAF3 leading to activation of downstream signal transduction of the type I IFN pathway (PubMed:32324863). Additionally, negatively regulates NLRP3/CASP1/IL-1beta-mediated pyroptosis and cell migration probably by ubiquitinating NLRP3 (PubMed:33724611).
Alternative names
RNF82, TIF1, TIF1A, TRIM24, Transcription intermediary factor 1-alpha, TIF1-alpha, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, RING-type E3 ubiquitin transferase TIF1-alpha, Tripartite motif-containing protein 24
Recommended products
Rabbit Recombinant Monoclonal TRIM24 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TRIM24 aa 1000 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human TRIM24 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O15164
- Clone number
- BLR225K
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRIM24 also known as transcription intermediary factor 1-alpha (TIF1α) is a protein that acts mechanically as a transcriptional regulator. It has a molecular mass of approximately 140 kDa. This protein contains several domains including a tripartite motif (the eponymous TRIM) which consists of a RING domain B-box domains and a coiled-coil region. TRIM24 interacts with nuclear receptors and is expressed in various tissues but shows higher expression in the liver and lungs. It also plays a role in bridging the interaction between chromatin and transcription factors.
- Biological function summary
TRIM24 is involved in transcriptional regulation by influencing gene expression. It acts as a co-regulator being part of large protein complexes. This protein interacts directly with histone tails to read histone marks and release transcriptional repression. TRIM24 also plays a role in ubiquitination and subsequent proteasomal degradation of specific proteins suggesting its involvement in maintaining protein homeostasis. Additionally TRIM24 has been shown to interact with p53 influencing cell cycle regulation and apoptosis.
- Pathways
TRIM24 is involved in the regulation of the retinoic acid and vitamin D signaling pathways. It modulates the transcriptional activity of nuclear receptors through these pathways influencing cell proliferation and differentiation processes. TRIM24 does so by interacting with other proteins such as retinoid X receptor (RXR) and estrogen receptor (ER) establishing important crosstalk between signaling cascades critical for cell fate decisions.
- Associated diseases and disorders
TRIM24 has been implicated in the development of certain cancers such as breast cancer and liver cancer. In these contexts it functions as an oncogene promoting tumorigenesis through aberrant transcriptional regulation. TRIM24 can interact with other proteins associated with oncogenesis including p53 modulating its tumor suppressor functions. Furthermore deregulation of TRIM24 activity or expression levels has been correlated with inflammatory disorders highlighting its relevance in both cancer and inflammation-related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Western blot - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (ab314090), expandable thumbnail](https://content.abcam.com/products/images/tif1-alpha-trim24-antibody-blr225k-bsa-free-ab314090--western-blot-img420592.jpg "Western blot - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (ab314090)")
Western blot - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (ab314090)
Samples: Whole cell lysate (10 µg) from HeLa, OVCAR-8, HEK293T, MOLT-4, and OVCAR-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TIF1 Alpha/TRIM24 recombinant monoclonal antibody [BLR225K] (A700-225) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
All lanes: Western blot - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (ab314090) at 1/1000 dilution
Lane 1: HeLa Whole cell lysate at 10 µg
Lane 2: OVCAR-8 Whole cell lysate at 10 µg
Lane 3: HEK293T Whole cell lysate at 10 µg
Lane 4: MOLT-4 Whole cell lysate at 10 µg
Lane 5: OVCAR-4 Whole cell lysate at 10 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Immunoprecipitation - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (ab314090), expandable thumbnail](https://content.abcam.com/products/images/tif1-alpha-trim24-antibody-blr225k-bsa-free-ab314090--immunoprecipitation-img420593.jpg "Immunoprecipitation - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (ab314090)")
Immunoprecipitation - Anti-TIF1 Alpha/TRIM24 antibody [BLR225K] - BSA free (ab314090)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-TIF1 Alpha/TRIM24 recombinant monoclonal antibody [BLR225K] (A700-225) used for IP at 20 µl/mg lysate. TIF1 Alpha/TRIM24 was also immunoprecipitated by a second antibody against a different epitope of TIF1 Alpha/TRIM24 (BL-2106A-1B4). For blotting immunoprecipitated TIF1 Alpha/TRIM24, A700-225 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.
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