Rabbit Recombinant Monoclonal TIFA antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (AB274326), expandable thumbnail](https://content.abcam.com/products/images/tifa-antibody-epr20925-114-bsa-and-azide-free-ab274326--western-blot-img23323.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (AB274326)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Adapter molecule that plays a key role in the activation of pro-inflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs) (PubMed:12566447, PubMed:15492226, PubMed:26068852, PubMed:28222186, PubMed:28877472, PubMed:30111836). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism (PubMed:15492226, PubMed:26068852). TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of pro-inflammatory NF-kappa-B signaling (PubMed:30111836).
Alternative names
T2BP, TIFA, TRAF-interacting protein with FHA domain-containing protein A, Putative MAPK-activating protein PM14, Putative NF-kappa-B-activating protein 20, TRAF2-binding protein
Recommended products
Rabbit Recombinant Monoclonal TIFA antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20925-114
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab274326 is the carrier-free version of Anti-TIFA antibody [EPR20925-114] ab239352.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TRAF-interacting protein with forkhead-associated domain (TIFA) also known as 55 kDa protein plays an important role in cellular signaling. It engages through interactions with TRAF2 and TRAF6 proteins which are involved in many cellular processes. TIFA has wide expression in several tissues including immune cells where it mediates immune responses. The protein localizes in the cytoplasm and its activities are often important in maintaining cellular homeostasis.
- Biological function summary
TIFA acts as a mediator in various cellular responses by forming signaling complexes. It possesses a threonine-phosphorylation motif necessary for the formation of oligomeric structures which facilitates its interactions with other proteins. Through this TIFA participates in immune response and inflammation regulation contributing to cellular resilience against external stresses.
- Pathways
TIFA is an important player in the NF-κB signaling and Toll-like receptor pathways. It regulates protein interactions within these pathways such as TRAF2 and TRAF6 that help propagate proinflammatory signals. The induction of TIFA in these pathways leads to the activation of downstream effectors which result in targeted immune responses.
- Associated diseases and disorders
TIFA functions have been linked with inflammatory disorders and type 1 diabetes. Dysregulation of TIFA may contribute to improper immune response leading to chronic inflammation seen in inflammatory conditions. It also interfaces with proteins like TRAF2 which are involved in metabolic disorders suggesting a role for TIFA in the pathogenesis of autoimmune diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (ab274326), expandable thumbnail](https://content.abcam.com/products/images/tifa-antibody-epr20925-114-bsa-and-azide-free-ab274326--western-blot-img23323.jpg "Western blot - Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (ab274326)")
Western blot - Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (ab274326)
This data was developed using Anti-TIFA antibody [EPR20925-114] ab239352, the same antibody clone in a different buffer formulation.
The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.
Anti-TIFA antibody [EPR20925-114] ab239352 Anti-TIFA antibody [EPR20925-114] was shown to react with TIFA in HEK-293T cells in Western blot. Loss of signal was observed when TIFA knockout sample was used. Wild-type and TIFA knockout samples were subjected to SDS-PAGE. Anti-TIFA antibody [EPR20925-114] ab239352 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.
Exposure time: 2 minutes.
All lanes: Western blot - Anti-TIFA antibody [EPR20925-114] (Anti-TIFA antibody [EPR20925-114] ab239352) at 1/1000 dilution
Lane 1: TIFA knockout HEK-293T whole cell lysate at 20 µg
Lane 2: Wild-type HEK-293T whole cell lysate at 20 µg
Lane 3: MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution
Predicted band size: 21 kDa
Observed band size: 20 kDa
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