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AB274326

Anti-TIFA antibody [EPR20925-114] - BSA and Azide free

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Rabbit Recombinant Monoclonal TIFA antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

T2BP, TIFA, TRAF-interacting protein with FHA domain-containing protein A, Putative MAPK-activating protein PM14, Putative NF-kappa-B-activating protein 20, TRAF2-binding protein

1 Images
Western blot - Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (AB274326)
  • WB

Collaborator

Western blot - Anti-TIFA antibody [EPR20925-114] - BSA and Azide free (AB274326)

This data was developed using ab239352, the same antibody clone in a different buffer formulation.

The image was kindly provided by our collaborator Dr Feng Shao's lab, NIBS.

ab239352 Anti-TIFA antibody [EPR20925-114] was shown to react with TIFA in HEK-293T cells in Western blot. Loss of signal was observed when TIFA knockout sample was used. Wild-type and TIFA knockout samples were subjected to SDS-PAGE. ab239352 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging. Anti-beta Tubulin was used as loading control.

Exposure time : 2 minutes.

All lanes:

Western blot - Anti-TIFA antibody [EPR20925-114] (<a href='/en-us/products/primary-antibodies/tifa-antibody-epr20925-114-ab239352'>ab239352</a>) at 1/1000 dilution

Lane 1:

TIFA knockout HEK-293T whole cell lysate at 20 µg

Lane 2:

Wild-type HEK-293T whole cell lysate at 20 µg

Lane 3:

MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

Predicted band size: 21 kDa

Observed band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20925-114

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab274326 is the carrier-free version of ab239352.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TRAF-interacting protein with forkhead-associated domain (TIFA) also known as 55 kDa protein plays an important role in cellular signaling. It engages through interactions with TRAF2 and TRAF6 proteins which are involved in many cellular processes. TIFA has wide expression in several tissues including immune cells where it mediates immune responses. The protein localizes in the cytoplasm and its activities are often important in maintaining cellular homeostasis.
Biological function summary

TIFA acts as a mediator in various cellular responses by forming signaling complexes. It possesses a threonine-phosphorylation motif necessary for the formation of oligomeric structures which facilitates its interactions with other proteins. Through this TIFA participates in immune response and inflammation regulation contributing to cellular resilience against external stresses.

Pathways

TIFA is an important player in the NF-κB signaling and Toll-like receptor pathways. It regulates protein interactions within these pathways such as TRAF2 and TRAF6 that help propagate proinflammatory signals. The induction of TIFA in these pathways leads to the activation of downstream effectors which result in targeted immune responses.

TIFA functions have been linked with inflammatory disorders and type 1 diabetes. Dysregulation of TIFA may contribute to improper immune response leading to chronic inflammation seen in inflammatory conditions. It also interfaces with proteins like TRAF2 which are involved in metabolic disorders suggesting a role for TIFA in the pathogenesis of autoimmune diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter molecule that plays a key role in the activation of pro-inflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs) (PubMed : 12566447, PubMed : 15492226, PubMed : 26068852, PubMed : 28222186, PubMed : 28877472, PubMed : 30111836). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism (PubMed : 15492226, PubMed : 26068852). TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria : ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of pro-inflammatory NF-kappa-B signaling (PubMed : 30111836).
See full target information TIFA
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Product promise

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