AB233404

Anti-TIGIT antibody

Name: Anti-TIGIT antibody (ab233404) | Abcam
Brand: Abcam Limited
SKU: AB233404
Price: 515 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal TIGIT antibody. Suitable for WB and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tigit aa 1 to C-terminus.

View Alternative Names

Vstm3, Tigit, T-cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3

2 Images

Supplier Data

Western blot - Anti-TIGIT antibody (AB233404)

All lanes:

Western blot - Anti-TIGIT antibody (ab233404) at 3 µg/mL

All lanes:

Mouse spleen tissue lysate

Secondary

All lanes:

HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 26 kDa

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Supplier Data

Western blot - Anti-TIGIT antibody (AB233404)

All lanes:

Western blot - Anti-TIGIT antibody (ab233404) at 2 µg/mL

All lanes:

Recombinant mouse TIGIT protein

Predicted band size: 26 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Recombinant Fragment Protein within Mouse Tigit aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P86176

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.

Biological function summary

TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.

Pathways

The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.

TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity.

See full target information Tigit

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107891 PubMed37766980

2023

Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Mick J M van Eijs,José J M Ter Linde,Matthijs J D Baars,Mojtaba Amini,Miangela M Laclé,Eelco C Brand,Eveline M Delemarre,Julia Drylewicz,Stefan Nierkens,Rik J Verheijden,Bas Oldenburg,Yvonne Vercoulen,Karijn P M Suijkerbuijk,Femke van Wijk

Communications medicine 3:60 PubMed37117276

2023

Digital morphometry and cluster analysis identifies four types of melanocyte during uveal melanoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Gustav Stålhammar,Viktor Torgny Gill

Cancer medicine 8:2784-2792 PubMed30993893

2019

Expression of immune checkpoint receptors Indoleamine 2,3-dioxygenase and T cell Ig and ITIM domain in metastatic versus nonmetastatic choroidal melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Gustav Stålhammar,Stefan Seregard,Hans E Grossniklaus

Oncoimmunology 8:e1593807 PubMed31069158

2019

Clinical significance of CD8 T cell immunoreceptor with Ig and ITIM domains in locally advanced gastric cancer treated with SOX regimen after D2 gastrectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Tang,Xiongxiong Pan,Di Han,Dawei Rong,Minghui Zhang,Lulu Yang,Jie Ying,Hua Guan,Ziyi Chen,Xuehao Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIGIT antibody

Western blot - Anti-TIGIT antibody (AB233404)

Western blot - Anti-TIGIT antibody (AB233404)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise