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Rabbit Recombinant Monoclonal TIGIT antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tigit aa 1-150.

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Images

Western blot - Anti-TIGIT antibody [BLR235K] - BSA free (AB314059), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Mouse Tigit aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P86176

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity.

Alternative names

Vstm3, Tigit, T-cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3

Recommended products

Rabbit Recombinant Monoclonal TIGIT antibody. Suitable for WB and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Tigit aa 1-150.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Mouse Tigit aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P86176
Clone number
BLR235K
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.

Biological function summary

TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.

Pathways

The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.

Associated diseases and disorders

TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.

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1 product image

  • Western blot - Anti-TIGIT antibody [BLR235K] - BSA free (ab314059), expandable thumbnail

    Western blot - Anti-TIGIT antibody [BLR235K] - BSA free (ab314059)

    Samples:Whole cell lysate (50 µg) from EL4, NIH 3T3, TCMK-1, ND7/23, and WEHI-231 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-TIGIT recombinant monoclonal antibody [BLR235K] (A700-235 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection:Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit antiActin recombinant monoclonal antibody [BLR057F] (A700-057).

    All lanes: Western blot - Anti-TIGIT antibody [BLR235K] - BSA free (ab314059) at 1/1000 dilution

    Lane 1: EL4 Whole cell lysate at 50 µg

    Lane 2: NIH 3T3 Whole cell lysate at 50 µg

    Lane 3: TCMK-1 Whole cell lysate at 50 µg

    Lane 4: ND7/23 Whole cell lysate at 50 µg

    Lane 5: WEHI-231 Whole cell lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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