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AB321793

Anti-TIGIT antibody [EPR28146-94]

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(3 Publications)

Rabbit Recombinant Monoclonal TIGIT antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples. Cited in 3 publications.

View Alternative Names

VSIG9, VSTM3, TIGIT, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-TIGIT antibody [EPR28146-94] (AB321793)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIGIT antibody [EPR28146-94] (AB321793)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HDLM-2 (human Hodgkin lymphoma cell) cells labelling TIGIT with ab321793 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Low expression : Confocal image showing no staining in HDLM-2 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)

Flow cytometric analysis of No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) (right) / PC-3 (human prostate adenocarcinoma epithelial cell) (Left) cells labelling TIGIT with ab321793 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : PC-3.

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TIGIT with ab321793 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD45RO conjugated to Pacific Blue.
Gated on viable CD3+ T Lymphocytes.

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TIGIT with ab321793 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD19 conjugated to PE/Cy7 (780/60BP).
Gated on viable leukomonocyte.

Immunocytochemistry/ Immunofluorescence - Anti-TIGIT antibody [EPR28146-94] (AB321793)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIGIT antibody [EPR28146-94] (AB321793)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) cells labelling TIGIT with ab321793 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing membranous staining in No-GFP-CD16.NK-92 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : PC-3.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TIGIT antibody [EPR28146-94] (AB321793)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIGIT antibody [EPR28146-94] (AB321793)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labelling TIGIT with ab321793 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in subsets of human PBMCs (shown in green), which is partial co-stain with CD3, the T cell maker. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-human CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TIGIT with ab321793 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD14 conjugated to Alexa Fluor®488.
Gated on viable cell.

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28146-94] (AB321793)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TIGIT with ab321793 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD56 conjugated to Alexa BV421 (440/50BP).
Gated on viable Lymphocytes.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28146-94

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.
Biological function summary

TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.

Pathways

The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.

TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed : 19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed : 23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed : 24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed : 32503945).
See full target information TIGIT
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40643462

2025

Insulin Modulates NK Cell Activity in Liver Fibrosis MASH Patients via the STING Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Amiram Ariel,Rifaat Safadi

CytoJournal 22:41 PubMed40469710

2025

Matrix gla protein mediates CD8 T-cell exhaustion to facilitate immune evasion in intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohan Cao,Shiqian Lang,Yuchi Xie,Kai Zheng,Jun Liu

Clinical cancer research : an official journal of the American Association for Cancer Research 31:2495-2514 PubMed40192737

2025

Single-Cell Transcriptomic Landscape Deciphers Intratumoral Heterogeneity and Subtypes of Acral and Mucosal Melanomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yunyan Li,Ziyang Cui,Xiaole Song,Yeqing Chen,Cang Li,Junfeng Shi,Wenkang Qian,Guoxin Ren,Jiang Zhou,Chunpu Li,Xiaoqing Ma,Yifan Chen,Dongdong Jia,Yongli Zhang,Zhilin Zhang,Ronghao Zhang,Zhaotian Zhang,Yong Chen,Zhixiang Xu,Wantao Chen,Xiao Miao,Hongmeng Yu,Jianxin Chen,Kai Wang,Colin R Goding,Zhi Wei,Tao Li,Rutao Cui
View all publications
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Complementary Products

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AB314598

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