JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB324719

Anti-TIGIT antibody [EPR28147-69] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TIGIT antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

Vstm3, Tigit, T-cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3

3 Images
Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] - BSA and Azide free (AB324719)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] - BSA and Azide free (AB324719)

This data was developed using ab324717, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cells were treated with 5ug/ml of immobilized anti-CD3 and anti-CD28 for 24 hours (Lower left and right) / Untreated control (Upper left and right) cells labelling TIGIT with ab324717 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD3 conjugated to Alexa Fluor® 647.

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] - BSA and Azide free (AB324719)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] - BSA and Azide free (AB324719)

This data was developed using ab324717, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cells were treated with 5ug/ml of immobilized anti-CD3 and anti-CD28 for 24 hours (Lower left and right) / Untreated control (Upper left and right) cells labelling TIGIT with ab324717 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD25 conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] - BSA and Azide free (AB324719)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TIGIT antibody [EPR28147-69] - BSA and Azide free (AB324719)

This data was developed using ab324717, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cells were treated with 5ug/ml of immobilized anti-CD3 and anti-CD28 for 24 hours (Lower left and right) / Untreated control (Upper left and right) cells labelling TIGIT with ab324717 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with CD19 conjugated to PE/Cy7.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28147-69

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

Product details

ab324719 is the carrier-free version of ab324717

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity.
See full target information Tigit
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com