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Rabbit Recombinant Monoclonal TIGIT antibody. Suitable for IHC-P, WB and reacts with Transfected cell line - Human, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (AB324237), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (AB324237), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (AB324237), expandable thumbnail
  • Western blot - Anti-TIGIT antibody [EPR29350-46] (AB324237), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (AB324237), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human
Dilution info
-
Notes

-

Target data

Function

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed:19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed:23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed:24817116). Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed:32503945).

Alternative names

VSIG9, VSTM3, TIGIT, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

Rabbit Recombinant Monoclonal TIGIT antibody. Suitable for IHC-P, WB and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29350-46
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for WB in human tissues.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237)

    TIGIT Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human tonsil using rabbit Anti-TIGIT antibody

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TIGIT with ab324237 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human tonsil. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237)

    TIGIT Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human pancreas using rabbit Anti-TIGIT antibody

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TIGIT with ab324237 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: no staining on human pancreas. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237)

    TIGIT Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human Hodgkin lymphoma using rabbit Anti-TIGIT antibody

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling TIGIT with ab324237 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human Hodgkin lymphoma. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-TIGIT antibody [EPR29350-46] (ab324237), expandable thumbnail

    Western blot - Anti-TIGIT antibody [EPR29350-46] (ab324237)

    TIGIT Western blot staining using rabbit Anti-TIGIT antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: 293T, PC-3.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TIGIT antibody [EPR29350-46] (ab324237) at 1/1000 dilution

    Lane 1: No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) whole cell lysate at 48 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 48 µg

    Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 48 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 30-50 kDa, 36 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237)

    TIGIT Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-TIGIT antibody

    Immunohistochemical analysis of paraffin-embedded A HEK-293T (human embryonic kidney epithelial cell) transfected with a human TIGIT expression vector containing a His tag. B HEK-293T transfected with empty vector containing a His tag. tissue labeling TIGIT with ab324237 at 1/500 (1.024 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on A: HEK-293T transfected with a human TIGIT expression vector containing a His tag, no staining on B: HEK-293T transfected with empty vector containing a His tag. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237)

    TIGIT Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human non-Hodgkin lymphoma using rabbit Anti-TIGIT antibody

    Immunohistochemical analysis of paraffin-embedded Human non-Hodgkin lymphoma tissue labeling TIGIT with ab324237 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on human non-Hodgkin lymphoma. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237)

    TIGIT Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human gastric carcinoma using rabbit Anti-TIGIT antibody

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling TIGIT with ab324237 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on stroma cells in human gastric carcinoma. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIGIT antibody [EPR29350-46] (ab324237)

    TIGIT Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Human kidney using rabbit Anti-TIGIT antibody

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TIGIT with ab324237 at 1/100 (5.12 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Negative control: no staining on human kidney. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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