AB252385

Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TIM 1 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD365, Kim1, Tim1, Timd1, Havcr1, Hepatitis A virus cellular receptor 1 homolog, HAVcr-1, Kidney injury molecule 1, T cell immunoglobulin and mucin domain-containing protein 1, T cell membrane protein 1, T-cell immunoglobulin mucin receptor 1, KIM-1, TIMD-1, TIM-1

1 Images

Flow Cytometry - Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (AB252385)

Flow Cyt

Unknown

Flow Cytometry - Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (AB252385)

Flow cytometric analysis of Mouse splenocytes (Left panel) / A20 (mouse reticulum sarcoma cell line, Right panel) labeling TIM 1 with ab252220 at 1/50 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Negative control : Mouse splenocytes (PMID : 26332704; PMID : 17277130).

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252220).

Unconjugated

Anti-TIM 1 antibody [EPR17561-22]
Carrier free

Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17561-22

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab252385 is the carrier-free version of ab252220.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.

Biological function summary

The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein’s activity influences cytokine production and this can affect inflammation processes.

Pathways

TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.

TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1’s involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions (PubMed : 21821911, PubMed : 25645598, PubMed : 32668241). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses (PubMed : 24703780). Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (PubMed : 24703780). Regulates also expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10 (PubMed : 25582854, PubMed : 25645598). Acts as a regulator of T-cell proliferation (PubMed : 15793576). May play a role in kidney injury and repair (By similarity).

See full target information Havcr1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced healthcare materials 12:e2300887 PubMed37317936

2023

Nanosized Silk-Magnesium Complexes for Tissue Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaozhao Ding,Weinan Cheng,Lutong Liu,Gang Xu,Qiang Lu,David L Kaplan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free

Flow Cytometry - Anti-TIM 1 antibody [EPR17561-22] - BSA and Azide free (AB252385)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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