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Rabbit Recombinant Monoclonal TIM 1 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples.

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Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Tested
Expected
Not recommended
Tested

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). May play a role in kidney injury and repair.(Microbial infection) Acts as a receptor for Hepatitis A virus.(Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed:21536871). Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP (PubMed:26487564).(Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane.(Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E.

Alternative names

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Rabbit Recombinant Monoclonal TIM 1 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR22650-136

Purification technique

Affinity purification Protein A

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab256484 is the carrier-free version of ab228973.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein’s activity influences cytokine production and this can affect inflammation processes.

Activity summary

TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.

Pathways

TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.

Associated diseases and disorders

TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1’s involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484), expandable thumbnail

    Flow Cytometry - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)

    Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labeling TIM 1 with ab228973 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control: HEK-293T (PMID: 23084921).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228973).

  • Immunocytochemistry/ Immunofluorescence - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (ab256484)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labeling TIM 1 with ab228973 at 1/100 (5.3μg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, 2μg/ml dilution (Green). Confocal image showing cytoplasmic punctate and membranous staining in Huh7 cell line (PMID:23233528) is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2μg/ml) dilution.

    Negative control: HEK-293T(PMID: 23084921).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228973).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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