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AB256484

Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TIM 1 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD365, KIM1, TIM1, TIMD1, HAVCR1, Hepatitis A virus cellular receptor 1, HAVcr-1, Kidney injury molecule 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell immunoglobulin mucin receptor 1, T-cell membrane protein 1, KIM-1, TIMD-1, TIM, TIM-1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (AB256484)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (AB256484)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Huh7 (human hepatocellular carcinoma epithelial cell) cells labeling TIM 1 with ab228973 at 1/100 (5.3μg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000, 2μg/ml dilution (Green). Confocal image showing cytoplasmic punctate and membranous staining in Huh7 cell line (PMID : 23233528) is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2μg/ml) dilution.

Negative control : HEK-293T（PMID : 23084921).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228973).

Flow Cytometry - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (AB256484)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (AB256484)

Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / Huh7 (human hepatocellular carcinoma epithelial cell, Right) cells labeling TIM 1 with ab228973 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Negative control : HEK-293T (PMID : 23084921).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab228973).

Western blot - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (AB256484)
  • WB

Lab

Western blot - Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (AB256484)

This data was developed using ab228973, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Please do not boil the lysate prior to loading it into the gel.<.p>

All lanes:

Western blot - Anti-TIM 1 antibody [EPR22650-136] (<a href='/en-us/products/primary-antibodies/tim-1-antibody-epr22650-136-ab228973'>ab228973</a>) at 1/1000 dilution

Lane 1:

Boiled Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Un-boiled Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa

false

Exposure time: 20s

  • Carrier free

    Anti-TIM 1 antibody [EPR22650-136] - BSA and Azide free (Detector)

  • Unconjugated

    Anti-TIM 1 antibody [EPR22650-136]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22650-136

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Please do not boil the lysate prior to loading it into the gel.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab256484 is the carrier-free version of ab228973.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.
Biological function summary

The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein’s activity influences cytokine production and this can affect inflammation processes.

Pathways

TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.

TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1’s involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphatidylserine receptor that plays an important functional role in regulatory B-cell homeostasis including generation, expansion and suppressor functions (By similarity). As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses (PubMed : 24703780). Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease (PubMed : 24703780). Also regulates expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10 (By similarity). Acts as a regulator of T-cell proliferation (By similarity). Confers phagocytic ability on injured kidney epithelial cells, allowing them to bind to and internalize apoptotic bodies and necrotic debris following kidney injury (PubMed : 18414680). The ectodomain binds to the surface of apoptotic kidney epithelial cells via phosphatidylserine and oxidized phospholipids exposed on the cell membrane of the apoptotic cells (PubMed : 18414680). HAVCR1-expressing cells are also capable of phagocytosing Gram-negative and Gram-positive bacteria (PubMed : 18414680).. (Microbial infection) Acts as a receptor for Hepatitis A virus.. (Microbial infection) Acts as a receptor for Ebolavirus and Marburg virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed : 21536871). Serves as a dual receptor for Ebolavirus by also interacting with envelope glycoprotein GP (PubMed : 26487564).. (Microbial infection) Acts as a receptor for Dengue virus by binding exposed phosphatidyl-serine at the surface of virion membrane (PubMed : 23084921). TIM1 and Dengue virus are co-internalized during virus entry (PubMed : 29742433).. (Microbial infection) Acts as a receptor for Zika virus by binding to envelope protein E.. (Microbial infection) Plays a positive role in Chikungunya virus cell entry.
See full target information HAVCR1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109471 PubMed38551005

2024

T cells with increased responsiveness cause obesity in mice without diet intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Ida Gregersen,Xiang Y Kong,Sander Kooijman,Håvard Foyn,Helene Grannes,Maria B Olsen,Anna M Lone,Kuan Yang,Ana Quiles-Jiménez,Marianne Tran,Jonas Øgaard,Filip M Segers,Azita Rashidi,Ellen Lund Sagen,Knut H Lauritzen,Amanda C M Pronk,Jan Freark de Boer,Kirsten B Holven,Espen Melum,Pål Aukrust,Kjetil Taskén,Sverre Holm,Patrick C N Rensen,Tuva B Dahl,Bente Halvorsen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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