AB47637

Anti-TIM 4 antibody

Name: Anti-TIM 4 antibody (ab47637) | Abcam
Brand: Abcam Limited
SKU: AB47637
Price: 460 USD
Availability: InStock

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(11 Publications)

Anti-TIM 4 antibody (ab47637) is a rabbit polyclonal antibody detecting TIM 4 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Trusted since 2007

View Alternative Names

TIM4, TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4

5 Images

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Western blot - Anti-TIM 4 antibody (AB47637)

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Western blot - Anti-TIM 4 antibody (ab47637) at 1 µg/mL

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Western blot - Anti-TIM 4 antibody (ab47637) at 2 µg/mL

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Jurkat cell lysate at 15 µg

Predicted band size: 42 kDa

Observed band size: 27 kDa,30 kDa,50 kDa,75 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody (AB47637)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody (AB47637)

ab47637 at 10μg/ml staining TIM 4 in Jurkat cells by ICC/IF

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody (AB47637)

Immunofluorescence of TIM-4 in Jurkat cells using ab47637 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody (AB47637)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody (AB47637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse liver tissue staining TIM 4 with ab47637 at 2µg/ml

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Western blot - Anti-TIM 4 antibody (AB47637)

Western Blot of RAW264.7 cell lysate labeling TIM 4 with Anti-TIM 4 antibody (ab47637) at 2μg/ml in (1) the absence and (2) the presence of blocking peptide.

All lanes:

Western blot - Anti-TIM 4 antibody (ab47637)

Predicted band size: 42 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TIMD4. The exact immunogen used to generate this antibody is proprietary information.

Q96H15

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10-20 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-TIM 4 antibody (ab47637) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of TIM 4?
Anti-TIM 4 (ab47637) specifically detects a band for TIM 4 (UniProt: Q96H15) at a molecular weight of 42kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM 4 also known as T cell immunoglobulin and mucin domain 4 is a protein of significant interest in immunology. It belongs to the TIM family of proteins which includes TIM 1 TIM 2 and TIM 3. TIM 4 weighs approximately 70 kDa and is mainly expressed on antigen-presenting cells including dendritic cells and macrophages. This protein plays an important role in immune response through its ability to recognize phosphatidylserine on apoptotic cells facilitating their clearance.

Biological function summary

The function of TIM 4 extends to regulating immune homeostasis and tolerance. TIM 4 does not possess signaling motifs itself but often associates with other adaptor proteins to exert its biological effects. It acts independently or forms part of complexes that modulate cell signaling events influencing the outcome of immune responses. The protein plays important roles in maintaining the delicate balance of immune activation and suppression which is critical to preventing autoimmunity.

Pathways

TIM 4 interaction occurs in pathways like the phosphatidylserine-mediated phagocytosis pathway which involves clearance of apoptotic cells. TIM 4 engages with phosphatidylserine a process important to maintaining tissue homeostasis and preventing inflammation. It works alongside other proteins in similar pathways including TIM 3 where together they mediate distinct and overlapping cellular processes involved in immune regulation.

TIM 4 presents significant implications in conditions including autoimmune diseases and cancer. Aberrant expression or function of TIM 4 associates with autoimmune disorders where it may contribute to the insufficient clearance of apoptotic cells subsequently promoting autoimmunity. In cancer altered TIM 4 activity can influence tumor progression by affecting immune evasion mechanisms. As TIM 4 interacts with TIM 3 these proteins together play roles in the development and progression of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation. Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways (By similarity). Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells (PubMed : 32703939, PubMed : 34067457). Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion (PubMed : 32640697). Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of LKB1/PRKAA1 pathway in a phosphatidylserine-dependent mechanism (By similarity).. (Microbial infection) Plays a positive role in exosome-mediated trafficking of HIV-1 virus and its entry into immune cells.

See full target information TIMD4

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 41:111738 PubMed36450259

2022

Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchun Wang,Yuzhen Wang,Lu Ding,Xiaolei Ren,Bo Wang,Liyuan Wang,Songbo Zhao,Xuetian Yue,Zhuanchang Wu,Chunyang Li,Xiaohong Liang,Chunhong Ma,Lifen Gao

Experimental and therapeutic medicine 24:679 PubMed36185763

2022

TIM4/NLRP3 axis participates in the effects of M1 macrophages on inflammatory factor release, apoptosis and cell adhesion in thyroid follicular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjun Cao,Yumeng Sun,Xiaowen Jin,Xiaoqian Li,Le Chen,Yan Qiu

Therapeutic advances in respiratory disease 15:17534666211039769 PubMed34852704

2021

Inhibition of LC3-associated phagocytosis in COPD and in response to cigarette smoke.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick F Asare,Hai B Tran,Plinio R Hurtado,Griffith B Perkins,Phan Nguyen,Hubertus Jersmann,Eugene Roscioli,Sandra Hodge

Journal of cellular and molecular medicine 24:1276-1285 PubMed31774937

2019

Inhibition of TIM-4 protects against cerebral ischaemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lifang Zheng,Yongqian Huang,Xinghua Wang,Xijia Wang,Wei Chen,Wei Cheng,Chunlian Pan

Journal for immunotherapy of cancer 6:145 PubMed30526680

2018

Tumor-derived exosomal HMGB1 fosters hepatocellular carcinoma immune evasion by promoting TIM-1 regulatory B cell expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Linsen Ye,Qi Zhang,Yusheng Cheng,Xiaolong Chen,Guoying Wang,Mengchen Shi,Tong Zhang,Yingjiao Cao,Hang Pan,Liting Zhang,Genshu Wang,Yinan Deng,Yang Yang,Guihua Chen

International journal of biological sciences 14:1186-1195 PubMed30123068

2018

Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Qiang Shen,Yuanxing Liu,Hui Chen,Xiaoxiao Zheng,Shangzhi Xie,Haofeng Ji,Shusen Zheng

PloS one 12:e0180143 PubMed28686604

2017

Boosting efferocytosis in alveolar space using BCG vaccine to protect host against influenza pneumonia.

Applications

Species

Mouse

Sanjay Mukherjee,Renuka Subramaniam,Han Chen,Anthony Smith,Shiva Keshava,Homayoun Shams

Nature communications 7:10871 PubMed26972991

2016

Stabilin-2 modulates the efficiency of myoblast fusion during myogenic differentiation and muscle regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Seung-Yoon Park,Youngeun Yun,Jung-Suk Lim,Mi-Jin Kim,Sang-Yeob Kim,Jung-Eun Kim,In-San Kim

British journal of cancer 113:1484-92 PubMed26512878

2015

TIM-4 promotes the growth of non-small-cell lung cancer in a RGD motif-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Zhang,Hongxing Wang,Xiaodong Wu,Bing Liu,Wen Liu,Rong Wang,Xiaohong Liang,Chunhong Ma,Lifen Gao

Journal of leukocyte biology 88:329-36 PubMed20453106

2010

T cell immunoglobulin- and mucin-domain-containing molecule-4 attenuates concanavalin A-induced hepatitis by regulating macrophage.

Applications

Species

Mouse

Liyun Xu,Jianni Qi,Peiqing Zhao,Xiaohong Liang,Ying Ju,Peng Liu,Bing Liu,Chun Guo,Lining Zhang,Chunhong Ma,Lifen Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TIM 4 antibody

Western blot - Anti-TIM 4 antibody (AB47637)

Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody (AB47637)

Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody (AB47637)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody (AB47637)

Western blot - Anti-TIM 4 antibody (AB47637)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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