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AB254208

Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free

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Rabbit Recombinant Monoclonal TIM 4 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.

View Alternative Names

TIM4, TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4

2 Images
Flow Cytometry - Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (AB254208)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (AB254208)

Flow cytometric analysis of 4% paraformaldehyde-fixed HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a myc-tagged TIM 4 expression construct labeling TIM 4 with ab222093 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue).

Cells were surface stained with ab222093. Then fixed with 2% PFA for 10min, followed by intracellular staining of anti-myc tag conjugated to Alexa Fluor®647. Gated on myc -positive population.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222093).

Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (AB254208)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR22304-3] - BSA and Azide free (AB254208)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TIM 4 transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells labeling TIM 4 (Green) with ab222093 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) antibody at 1/1000 dilution. Confocal image showing positive staining in HEK-293T cells transfected with a myc-tagged TIM 4 expression construct. myc-Tag (9B11) Mouse mAb (Alexa Fluor® 594 Conjugate) (ab223894) was used as the counterstain (Red) at 1/100 dilution. The nuclear counterstain was DAPI (Blue).

Negative control : Myc only transfected HEK-293T cells.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222093).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22304-3

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab254208 is the carrier-free version of ab222093.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM 4 also known as T cell immunoglobulin and mucin domain 4 is a protein of significant interest in immunology. It belongs to the TIM family of proteins which includes TIM 1 TIM 2 and TIM 3. TIM 4 weighs approximately 70 kDa and is mainly expressed on antigen-presenting cells including dendritic cells and macrophages. This protein plays an important role in immune response through its ability to recognize phosphatidylserine on apoptotic cells facilitating their clearance.
Biological function summary

The function of TIM 4 extends to regulating immune homeostasis and tolerance. TIM 4 does not possess signaling motifs itself but often associates with other adaptor proteins to exert its biological effects. It acts independently or forms part of complexes that modulate cell signaling events influencing the outcome of immune responses. The protein plays important roles in maintaining the delicate balance of immune activation and suppression which is critical to preventing autoimmunity.

Pathways

TIM 4 interaction occurs in pathways like the phosphatidylserine-mediated phagocytosis pathway which involves clearance of apoptotic cells. TIM 4 engages with phosphatidylserine a process important to maintaining tissue homeostasis and preventing inflammation. It works alongside other proteins in similar pathways including TIM 3 where together they mediate distinct and overlapping cellular processes involved in immune regulation.

TIM 4 presents significant implications in conditions including autoimmune diseases and cancer. Aberrant expression or function of TIM 4 associates with autoimmune disorders where it may contribute to the insufficient clearance of apoptotic cells subsequently promoting autoimmunity. In cancer altered TIM 4 activity can influence tumor progression by affecting immune evasion mechanisms. As TIM 4 interacts with TIM 3 these proteins together play roles in the development and progression of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation. Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways (By similarity). Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells (PubMed : 32703939, PubMed : 34067457). Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion (PubMed : 32640697). Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of LKB1/PRKAA1 pathway in a phosphatidylserine-dependent mechanism (By similarity).. (Microbial infection) Plays a positive role in exosome-mediated trafficking of HIV-1 virus and its entry into immune cells.
See full target information TIMD4
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Product promise

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For full details, please see our Terms & Conditions

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