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AB307558

Anti-TIM 4 antibody [EPR26194-8]

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(1 Publication)

Rabbit Recombinant Monoclonal TIM 4 antibody. Suitable for ICC/IF and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Tim4, Timd4, T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, SMUCKLER, TIM-4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] (AB307558)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] (AB307558)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized NIH/3T3 (mouse embryonic fibroblast) cells lABelling TIM 4 with ab307558 at 1/50 (10.66 ug/ml) dilution, followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing no staining in NIH/3T3 cell line.Negative control : NIH/3T3 (PMID : 28768810).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2ug/ml dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] (AB307558)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] (AB307558)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized RAW 264.7 (mouse ABelson murine leukemia virus-induced tumor macrophage) cells lABelling TIM 4 with ab307558 at 1/50 (10.66 ug/ml) dilution, followed by ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in RAW 264.7 cells treated with lipopolysaccharide (100ng/ml) for 16 h.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Carrier free

    Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26194-8

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM 4 also known as T cell immunoglobulin and mucin domain 4 is a protein of significant interest in immunology. It belongs to the TIM family of proteins which includes TIM 1 TIM 2 and TIM 3. TIM 4 weighs approximately 70 kDa and is mainly expressed on antigen-presenting cells including dendritic cells and macrophages. This protein plays an important role in immune response through its ability to recognize phosphatidylserine on apoptotic cells facilitating their clearance.
Biological function summary

The function of TIM 4 extends to regulating immune homeostasis and tolerance. TIM 4 does not possess signaling motifs itself but often associates with other adaptor proteins to exert its biological effects. It acts independently or forms part of complexes that modulate cell signaling events influencing the outcome of immune responses. The protein plays important roles in maintaining the delicate balance of immune activation and suppression which is critical to preventing autoimmunity.

Pathways

TIM 4 interaction occurs in pathways like the phosphatidylserine-mediated phagocytosis pathway which involves clearance of apoptotic cells. TIM 4 engages with phosphatidylserine a process important to maintaining tissue homeostasis and preventing inflammation. It works alongside other proteins in similar pathways including TIM 3 where together they mediate distinct and overlapping cellular processes involved in immune regulation.

TIM 4 presents significant implications in conditions including autoimmune diseases and cancer. Aberrant expression or function of TIM 4 associates with autoimmune disorders where it may contribute to the insufficient clearance of apoptotic cells subsequently promoting autoimmunity. In cancer altered TIM 4 activity can influence tumor progression by affecting immune evasion mechanisms. As TIM 4 interacts with TIM 3 these proteins together play roles in the development and progression of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation (PubMed : 17960135). Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways. Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells (PubMed : 18354194, PubMed : 18367551). Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion (By similarity). Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of STK11/PRKAA1 pathway in a phosphatidylserine-dependent mechanism (PubMed : 31263038).
See full target information Timd4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Basic research in cardiology 119:243-260 PubMed38329499

2024

Mineralocorticoid receptor promotes cardiac macrophage inflammaging.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Fraccarollo,Robert Geffers,Paolo Galuppo,Johann Bauersachs
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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