Rabbit Recombinant Monoclonal TIM 4 antibody. Carrier free. Suitable for ICC/IF and reacts with Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (AB307559), expandable thumbnail](https://content.abcam.com/products/images/tim-4-antibody-epr26194-8-bsa-and-azide-free-ab307559--immunocytochemistry-immunofluorescence-img405727.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (AB307559)")
![Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (AB307559), expandable thumbnail](https://content.abcam.com/products/images/tim-4-antibody-epr26194-8-bsa-and-azide-free-ab307559--immunocytochemistry-immunofluorescence-img405726.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (AB307559)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Target data
Function
Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation (PubMed:17960135). Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways. Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells (PubMed:18354194, PubMed:18367551). Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion (By similarity). Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of STK11/PRKAA1 pathway in a phosphatidylserine-dependent mechanism (PubMed:31263038).
Alternative names
Tim4, Timd4, T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, SMUCKLER, TIM-4
Recommended products
Rabbit Recombinant Monoclonal TIM 4 antibody. Carrier free. Suitable for ICC/IF and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26194-8
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TIM 4 also known as T cell immunoglobulin and mucin domain 4 is a protein of significant interest in immunology. It belongs to the TIM family of proteins which includes TIM 1 TIM 2 and TIM 3. TIM 4 weighs approximately 70 kDa and is mainly expressed on antigen-presenting cells including dendritic cells and macrophages. This protein plays an important role in immune response through its ability to recognize phosphatidylserine on apoptotic cells facilitating their clearance.
- Biological function summary
The function of TIM 4 extends to regulating immune homeostasis and tolerance. TIM 4 does not possess signaling motifs itself but often associates with other adaptor proteins to exert its biological effects. It acts independently or forms part of complexes that modulate cell signaling events influencing the outcome of immune responses. The protein plays important roles in maintaining the delicate balance of immune activation and suppression which is critical to preventing autoimmunity.
- Pathways
TIM 4 interaction occurs in pathways like the phosphatidylserine-mediated phagocytosis pathway which involves clearance of apoptotic cells. TIM 4 engages with phosphatidylserine a process important to maintaining tissue homeostasis and preventing inflammation. It works alongside other proteins in similar pathways including TIM 3 where together they mediate distinct and overlapping cellular processes involved in immune regulation.
- Associated diseases and disorders
TIM 4 presents significant implications in conditions including autoimmune diseases and cancer. Aberrant expression or function of TIM 4 associates with autoimmune disorders where it may contribute to the insufficient clearance of apoptotic cells subsequently promoting autoimmunity. In cancer altered TIM 4 activity can influence tumor progression by affecting immune evasion mechanisms. As TIM 4 interacts with TIM 3 these proteins together play roles in the development and progression of these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (ab307559), expandable thumbnail](https://content.abcam.com/products/images/tim-4-antibody-epr26194-8-bsa-and-azide-free-ab307559--immunocytochemistry-immunofluorescence-img405727.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (ab307559)")
Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (ab307559)
This data was developed using Anti-TIM 4 antibody [EPR26194-8] ab307558, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized RAW 264.7 (mouse ABelson murine leukemia virus-induced tumor macrophage) cells lABelling TIM 4 with Anti-TIM 4 antibody [EPR26194-8] ab307558 at 1/50 (10.66 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in RAW 264.7 cells treated with lipopolysaccharide (100ng/ml) for 16 h.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2ug/ml dilution.![Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (ab307559), expandable thumbnail](https://content.abcam.com/products/images/tim-4-antibody-epr26194-8-bsa-and-azide-free-ab307559--immunocytochemistry-immunofluorescence-img405726.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (ab307559)")
Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody [EPR26194-8] - BSA and Azide free (ab307559)
This data was developed using Anti-TIM 4 antibody [EPR26194-8] ab307558, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeABilized NIH/3T3 (mouse embryonic fibroblast) cells lABelling TIM 4 with Anti-TIM 4 antibody [EPR26194-8] ab307558 at 1/50 (10.66 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing no staining in NIH/3T3 cell line.Negative control: NIH/3T3 (PMID: 28768810).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2ug/ml dilution.
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