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Rabbit Recombinant Monoclonal TIM 4 antibody. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human samples.

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Images

Dot Blot - Anti-TIM 4 antibody [EPR29500-15] (AB323572), expandable thumbnail
  • Western blot - Anti-TIM 4 antibody [EPR29500-15] (AB323572), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (AB323572), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (AB323572), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (AB323572), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIHC-PWB
Human
Expected
Tested
Tested
Transfected cell lysate - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

Phosphatidylserine receptor that plays different role in immune response including phagocytosis of apoptotic cells and T-cell regulation. Controls T-cell activation in a bimodal fashion, decreasing the activation of naive T-cells by inducing cell cycle arrest, while increasing proliferation of activated T-cells by activating AKT1 and ERK1/2 phosphorylations and subsequent signaling pathways (By similarity). Also plays a role in efferocytosis which is the process by which apoptotic cells are removed by phagocytic cells (PubMed:32703939, PubMed:34067457). Mechanistically, promotes the engulfment of apoptotic cells or exogenous particles by securing them to phagocytes through direct binding to phosphatidylserine present on apoptotic cells, while other engulfment receptors such as MERTK efficiently recognize apoptotic cells and mediate their ingestion (PubMed:32640697). Additionally, promotes autophagy process by suppressing NLRP3 inflammasome activity via activation of LKB1/PRKAA1 pathway in a phosphatidylserine-dependent mechanism (By similarity). (Microbial infection) Plays a positive role in exosome-mediated trafficking of HIV-1 virus and its entry into immune cells.

Alternative names

TIM4, TIMD4, T-cell immunoglobulin and mucin domain-containing protein 4, TIMD-4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4

Rabbit Recombinant Monoclonal TIM 4 antibody. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29500-15
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Dot Blot - Anti-TIM 4 antibody [EPR29500-15] (ab323572), expandable thumbnail

    Dot Blot - Anti-TIM 4 antibody [EPR29500-15] (ab323572)

    Dot blot analysis of TIM 4 using ab323572 at 1:1000 (0.496 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: 293T cells transfected with a human TIM 4 expression vector containing a myc-His-tag®, whole cell lysate 10 ng

    Lane2: 293T cells transfected with a human TIM 1 expression vector containing a myc-His-tag®, whole cell lysate 500 ng

    Exposure time: 180 seconds.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human TIM 1.

    #In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Dot Blot - Anti-TIM 4 antibody [EPR29500-15] (ab323572) at 1/1000 dilution

    Lane 1: 293T cells transfected with a human TIM 4 expression vector containing a myc-His-tag®, whole cell lysate at 10 ng

    Lane 2: 293T cells transfected with a human TIM 1 expression vector containing a myc-His-tag®, whole cell lysate at 500 ng

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-TIM 4 antibody [EPR29500-15] (ab323572), expandable thumbnail

    Western blot - Anti-TIM 4 antibody [EPR29500-15] (ab323572)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: liver, tetsis (PMID: 14768054; PMID: 17960135)

    The samples are unboiled.

    Lane 1 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    The identity of the bands lower than 50 kDa in lanes 1-2 are unknown.

    The identity of the bands higher than 50 kDa in lane 1 are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1: 126 seconds; Lanes 2-4: 180 seconds.

    All lanes: Western blot - Anti-TIM 4 antibody [EPR29500-15] (ab323572) at 1/1000 dilution

    Lane 1: Human lymphoma tissue lysate at 20 µg

    Lane 2: Human tonsil tissue lysate at 20 µg

    Lane 3: Human liver tissue lysate at 20 µg

    Lane 4: Human testis tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 50 kDa, 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human cardiac muscle.

    The section was incubated with ab323572 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human tonsil.

    The section was incubated with ab323572 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: Weak staining on human liver.

    The section was incubated with ab323572 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in immunocytes of human colon.

    The section was incubated with ab323572 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (ab323572)

    Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human cervical carcinoma.

    The section was incubated with ab323572 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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