Anti-TIM 4 antibody [EPR29500-15]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (AB323572)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : Weak staining on human liver.
The section was incubated with ab323572 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (AB323572)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human cardiac muscle.
The section was incubated with ab323572 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (AB323572)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human tonsil.
The section was incubated with ab323572 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (AB323572)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human cervical carcinoma.
The section was incubated with ab323572 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody [EPR29500-15] (AB323572)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TIM 4 with ab323572 at 1/200 (2.48 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in immunocytes of human colon.
The section was incubated with ab323572 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-TIM 4 antibody [EPR29500-15] (AB323572)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver, tetsis (PMID : 14768054; PMID : 17960135)

The samples are unboiled.

Lane 1 of this blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

The identity of the bands lower than 50 kDa in lanes 1-2 are unknown.

The identity of the bands higher than 50 kDa in lane 1 are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1 : 126 seconds; Lanes 2-4 : 180 seconds.

All lanes:

Western blot - Anti-TIM 4 antibody [EPR29500-15] (ab323572) at 1/1000 dilution

Lane 1:

Human lymphoma tissue lysate at 20 µg

Lane 2:

Human tonsil tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 50 kDa,36 kDa

false

• Dot

Supplier Data

Dot Blot - Anti-TIM 4 antibody [EPR29500-15] (AB323572)

Dot blot analysis of TIM 4 using ab323572 at 1 : 1000 (0.496 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : 293T cells transfected with a human TIM 4 expression vector containing a myc-His-tag®, whole cell lysate 10 ng
Lane2 : 293T cells transfected with a human TIM 1 expression vector containing a myc-His-tag®, whole cell lysate 500 ng

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human TIM 1.

#In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-TIM 4 antibody [EPR29500-15] (ab323572) at 1/1000 dilution

Lane 1:

293T cells transfected with a human TIM 4 expression vector containing a myc-His-tag®, whole cell lysate at 10 ng

Lane 2:

293T cells transfected with a human TIM 1 expression vector containing a myc-His-tag®, whole cell lysate at 500 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s