AB167423

Anti-TIM22 antibody [EPR9973]

Name: Anti-TIM22 antibody [EPR9973] (ab167423) | Abcam
Brand: Abcam Limited
SKU: AB167423
Price: 325 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal TIM22 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

TEX4, TIM22, TIMM22, Mitochondrial import inner membrane translocase subunit Tim22, Testis-expressed protein 4

1 Images

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Western blot - Anti-TIM22 antibody [EPR9973] (AB167423)

All lanes:

Western blot - Anti-TIM22 antibody [EPR9973] (ab167423) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

T47D cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 20 kDa

false

Carrier free

Anti-TIM22 antibody [EPR9973] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9973

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TIM22 protein also known as Translocase of Inner Mitochondrial Membrane 22 has a molecular mass of approximately 20 kilodaltons. It plays an important mechanical role in the import and insertion of membrane proteins into the mitochondrial inner membrane. TIM22 locates itself in the mitochondrial inner membrane where it functions as part of a larger translocase complex.

Biological function summary

TIM22 interacts with several mitochondria-rich cellular environments to facilitate the integration of polytopic proteins. As part of the TIM22 complex it integrates endeavored proteins into the mitochondrial inner membrane. This integration is essential for the optimal function of the mitochondria. It interacts closely with other components including TIM54 and TIM18 which assist in its function within the complex.

Pathways

The TIM22 complex activities heavily contribute to the mitochondrial import pathways. It remains connected with the translocase of outer mitochondrial membrane (TOM) complex as it receives incoming proteins. TIM22 connects with TOM40 and small TIM such as TIM9 and TIM10 during the import and sorting processes. This integration ensures that proteins achieve the correct membrane insertion needed for mitochondrial function.

TIM22-related dysfunction can lead to mitochondrial-related disorders such as mitochondrial myopathy. This happens when the protein integration into the mitochondrial membrane faces disruption. Additionally deficiencies or mutations in TIM22 have links to neurodegenerative disorders where proper mitochondrial function is compromised. The interaction with other members of the mitochondrial membrane translocase complexes like TIM54 and TIM18 is important in these disorder contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps (By similarity).

See full target information TIMM22

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 34:108869 PubMed33730581

2021

The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle Grace Acoba,Ebru S Selen Alpergin,Santosh Renuse,Lucía Fernández-Del-Río,Ya-Wen Lu,Oleh Khalimonchuk,Catherine F Clarke,Akhilesh Pandey,Michael J Wolfgang,Steven M Claypool

Journal of cellular biochemistry 122:626-638 PubMed33522619

2021

The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhei Ishikura,Masayoshi Nagai,Toshiyuki Tsunoda,Kensuke Nishi,Yoko Tanaka,Midori Koyanagi,Senji Shirasawa

Scientific reports 9:2822 PubMed30808977

2019

Mieap-induced accumulation of lysosomes within mitochondria (MALM) regulates gastric cancer cell invasion under hypoxia by suppressing reactive oxygen species accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichiro Okuyama,Yoshihiko Kitajima,Noriyuki Egawa,Hiroshi Kitagawa,Kotaro Ito,Shinichi Aishima,Kazuyoshi Yanagihara,Tomokazu Tanaka,Hirokazu Noshiro

Stem cell reviews and reports 15:427-438 PubMed30338499

2018

Metabolic Syndrome Modulates Protein Import into the Mitochondria of Porcine Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Arash Aghajani Nargesi,Xiang-Yang Zhu,LaTonya J Hickson,Sabena M Conley,Andre J van Wijnen,Lilach O Lerman,Alfonso Eirin

American journal of human genetics 99:860-876 PubMed27693233

2016

Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle Thompson,Homa Majd,Cristina Dallabona,Karit Reinson,Martin S King,Charlotte L Alston,Langping He,Tiziana Lodi,Simon A Jones,Aviva Fattal-Valevski,Nitay D Fraenkel,Ann Saada,Alon Haham,Pirjo Isohanni,Roshni Vara,Inês A Barbosa,Michael A Simpson,Charu Deshpande,Sanna Puusepp,Penelope E Bonnen,Richard J Rodenburg,Anu Suomalainen,Katrin Õunap,Orly Elpeleg,Ileana Ferrero,Robert McFarland,Edmund R S Kunji,Robert W Taylor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIM22 antibody [EPR9973]

Western blot - Anti-TIM22 antibody [EPR9973] (AB167423)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise