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AB168649

Anti-TIM44 antibody

2

(2 Reviews)

|

(1 Publication)

Mouse Polyclonal TIM44 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TIMM44.

View Alternative Names

MIMT44, TIM44, TIMM44, Mitochondrial import inner membrane translocase subunit TIM44

1 Images
Western blot - Anti-TIM44 antibody (AB168649)
  • WB

Unknown

Western blot - Anti-TIM44 antibody (AB168649)

All lanes:

Western blot - Anti-TIM44 antibody (ab168649) at 1 µg/mL

Lane 1:

TIMM44 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 51 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TIMM44.

O43615

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 99% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM44 also known as translocase of inner mitochondrial membrane 44 serves an important role in mitochondrial protein import mechanisms. The protein weighs approximately 44 kDa and localizes in the inner mitochondrial membrane. TIM44 works as an essential part of the mitochondrial inner membrane presequence translocase complex where it assists in guiding proteins into the mitochondrial matrix. Expression of TIM44 is observed mainly in tissues with high energetic demands such as muscle and liver.
Biological function summary

TIM44 functions in conjunction with other components of the TIM23 complex which includes proteins like TIM23 and TIM17. The main role of TIM44 involves connecting the translocase complex to mitochondrial heat shock protein 70 (mtHsp70) facilitating ATP-dependent translocation of polypeptides. This process supports the import of precursor proteins synthesized in the cytosol into the mitochondria ensuring proper mitochondrial function and energy production.

Pathways

TIM44 influences the mitochondrial protein import pathway and the maintenance of mitochondrial function. As part of the protein import machinery it connects closely to the chaperone-mediated folding pathway aided by mtHsp70. The TIM23 complex participates in the translocation of proteins with mitochondrial targeting sequences linking TIM44 to pathways essential for mitochondrial biogenesis and function.

Dysfunction in the TIM44 protein associates with mitochondrial disorders including those impacting oxidative phosphorylation capacity. Mutations or deficiencies in TIM44 have connections to disorders such as mitochondrial myopathy. Relation to other proteins such as mtHsp70 and components of oxidative phosphorylation emphasizes the role of TIM44 in cellular energy homeostasis and highlights its importance in maintaining mitochondrial health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (By similarity). Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source (By similarity).
See full target information TIMM44
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 14:403-410 PubMed26774479

2016

EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter.

Applications

Unspecified application

Species

Unspecified reactive species

Horia Vais,Karthik Mallilankaraman,Don-On Daniel Mak,Henry Hoff,Riley Payne,Jessica E Tanis,J Kevin Foskett
View all publications
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Product promise

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