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AB244466

Anti-TIM44 antibody

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(2 Publications)

Rabbit Polyclonal TIM44 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TIMM44 aa 100-250.

View Alternative Names

MIMT44, TIM44, TIMM44, Mitochondrial import inner membrane translocase subunit TIM44

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (AB244466)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (AB244466)

Paraffin-embedded human cerebral cortex tissue stained for TIM44 using ab244466 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (AB244466)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (AB244466)

Paraffin-embedded human heart muscle tissue stained for TIM44 using ab244466 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (AB244466)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (AB244466)

Paraffin-embedded human testis tissue stained for TIM44 using ab244466 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-TIM44 antibody (AB244466)
  • WB

Unknown

Western blot - Anti-TIM44 antibody (AB244466)

All lanes:

Western blot - Anti-TIM44 antibody (ab244466) at 0.4 µg/mL

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with siRNA probe#1

Lane 2:

U-2 OS cells transfected with siRNA probe#2

Lane 3:

U-2 OS cells transfected with siRNA control

Predicted band size: 51 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (AB244466)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody (AB244466)

Paraffin-embedded human gastrointestinal tissue stained for TIM44 using ab244466 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody (AB244466)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody (AB244466)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for TIM44 (green) using ab244466 at 4 μg/ml in ICC/IF.

Western blot - Anti-TIM44 antibody (AB244466)
  • WB

Unknown

Western blot - Anti-TIM44 antibody (AB244466)

All lanes:

Western blot - Anti-TIM44 antibody (ab244466) at 0.4 µg/mL

Lane 1:

PC-3 (human prostate adenocarcinoma cell line) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 51 kDa

false

Western blot - Anti-TIM44 antibody (AB244466)
  • WB

Unknown

Western blot - Anti-TIM44 antibody (AB244466)

All lanes:

Western blot - Anti-TIM44 antibody (ab244466) at 0.4 µg/mL

Lane 1:

Caco-2 (Human colorectal adenocarcinoma cell line) cells transfected with a siRNA control

Lane 2:

Caco-2 cells transfected with a siRNA control

Lane 3:

Caco-2 cells transfected with a siRNA probe#2

Predicted band size: 51 kDa

false

Western blot - Anti-TIM44 antibody (AB244466)
  • WB

Unknown

Western blot - Anti-TIM44 antibody (AB244466)

All lanes:

Western blot - Anti-TIM44 antibody (ab244466) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II whole cell lysate

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TIMM44 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

O43615

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM44 also known as translocase of inner mitochondrial membrane 44 serves an important role in mitochondrial protein import mechanisms. The protein weighs approximately 44 kDa and localizes in the inner mitochondrial membrane. TIM44 works as an essential part of the mitochondrial inner membrane presequence translocase complex where it assists in guiding proteins into the mitochondrial matrix. Expression of TIM44 is observed mainly in tissues with high energetic demands such as muscle and liver.
Biological function summary

TIM44 functions in conjunction with other components of the TIM23 complex which includes proteins like TIM23 and TIM17. The main role of TIM44 involves connecting the translocase complex to mitochondrial heat shock protein 70 (mtHsp70) facilitating ATP-dependent translocation of polypeptides. This process supports the import of precursor proteins synthesized in the cytosol into the mitochondria ensuring proper mitochondrial function and energy production.

Pathways

TIM44 influences the mitochondrial protein import pathway and the maintenance of mitochondrial function. As part of the protein import machinery it connects closely to the chaperone-mediated folding pathway aided by mtHsp70. The TIM23 complex participates in the translocation of proteins with mitochondrial targeting sequences linking TIM44 to pathways essential for mitochondrial biogenesis and function.

Dysfunction in the TIM44 protein associates with mitochondrial disorders including those impacting oxidative phosphorylation capacity. Mutations or deficiencies in TIM44 have connections to disorders such as mitochondrial myopathy. Relation to other proteins such as mtHsp70 and components of oxidative phosphorylation emphasizes the role of TIM44 in cellular energy homeostasis and highlights its importance in maintaining mitochondrial health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (By similarity). Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source (By similarity).
See full target information TIMM44
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 40:108 PubMed39630363

2024

The mechanism of sevoflurane affecting ovarian cancer cell proliferation and migration by regulating RNA methylase TRDMT1 to activate the β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Huang,Xuewei Lao,Chengyan He,Jia Wang,Ying Pan

Nature communications 11:4116 PubMed32807793

2020

OSMR controls glioma stem cell respiration and confers resistance of glioblastoma to ionizing radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmad Sharanek,Audrey Burban,Matthew Laaper,Emilie Heckel,Jean-Sebastien Joyal,Vahab D Soleimani,Arezu Jahani-Asl
View all publications
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