AB232592

Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TIM44 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

MIMT44, TIM44, TIMM44, Mitochondrial import inner membrane translocase subunit TIM44

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling TIM44 with ab201453 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on K562 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows :
-ve control 1 : ab201453 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201453).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling TIM44 with ab201453 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201453).

Flow Cytometry (Intracellular) - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) labelling TIM44 with purified ab201453 at 1/500 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor^® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201453).

Supplier Data

Immunoprecipitation - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

TIM44 was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate with ab201453 at 1/120 dilution. Western blot was performed from the immunoprecipitate using ab201453 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

Lane 1 : K562 whole cell lysate 10 μg (Input). Lane 2 : ab201453 IP in K562 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab201453 in K562 whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201453).

All lanes:

Immunoprecipitation - Anti-TIM44 antibody [EPR16821-26] (<a href='/en-us/products/primary-antibodies/tim44-antibody-epr16821-26-ab201453'>ab201453</a>)

Predicted band size: 51 kDa

false

Unconjugated

Anti-TIM44 antibody [EPR16821-26]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16821-26

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab232592 is the carrier-free version of ab201453.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM44 also known as translocase of inner mitochondrial membrane 44 serves an important role in mitochondrial protein import mechanisms. The protein weighs approximately 44 kDa and localizes in the inner mitochondrial membrane. TIM44 works as an essential part of the mitochondrial inner membrane presequence translocase complex where it assists in guiding proteins into the mitochondrial matrix. Expression of TIM44 is observed mainly in tissues with high energetic demands such as muscle and liver.

Biological function summary

TIM44 functions in conjunction with other components of the TIM23 complex which includes proteins like TIM23 and TIM17. The main role of TIM44 involves connecting the translocase complex to mitochondrial heat shock protein 70 (mtHsp70) facilitating ATP-dependent translocation of polypeptides. This process supports the import of precursor proteins synthesized in the cytosol into the mitochondria ensuring proper mitochondrial function and energy production.

Pathways

TIM44 influences the mitochondrial protein import pathway and the maintenance of mitochondrial function. As part of the protein import machinery it connects closely to the chaperone-mediated folding pathway aided by mtHsp70. The TIM23 complex participates in the translocation of proteins with mitochondrial targeting sequences linking TIM44 to pathways essential for mitochondrial biogenesis and function.

Dysfunction in the TIM44 protein associates with mitochondrial disorders including those impacting oxidative phosphorylation capacity. Mutations or deficiencies in TIM44 have connections to disorders such as mitochondrial myopathy. Relation to other proteins such as mtHsp70 and components of oxidative phosphorylation emphasizes the role of TIM44 in cellular energy homeostasis and highlights its importance in maintaining mitochondrial health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (By similarity). Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source (By similarity).

See full target information TIMM44

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 7:493-507 PubMed39972217

2025

Pro-inflammatory macrophages produce mitochondria-derived superoxide by reverse electron transport at complex I that regulates IL-1β release during NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Alva M Casey,Dylan G Ryan,Hiran A Prag,Suvagata Roy Chowdhury,Eloïse Marques,Keira Turner,Anja V Gruszczyk,Ming Yang,Dane M Wolf,Jan Lj Miljkovic,Joyce Valadares,Patrick F Chinnery,Richard C Hartley,Christian Frezza,Julien Prudent,Michael P Murphy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

Flow Cytometry (Intracellular) - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

Immunoprecipitation - Anti-TIM44 antibody [EPR16821-26] - BSA and Azide free (AB232592)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise