AB194829

Anti-TIM44 antibody [EPR16821]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal TIM44 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 7 publications.

View Alternative Names

MIMT44, TIM44, TIMM44, Mitochondrial import inner membrane translocase subunit TIM44

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] (AB194829)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] (AB194829)

Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling TIM44 with ab194829 at 1/100 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain : Hematoxylin.
Inset image : negative control obtained using PBS instead of ab194829. Cytoplasm staining on humanendometrial adenocarcinoma was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] (AB194829)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] (AB194829)

Immunofluorescent analysis of A431 cells labeling TIM44 with ab194829 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain : DAPI. Cytoplasm staining on A431 cell line was observed.

Supplier Data

Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (AB194829)

Immunoprecipitation analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab194826 at 1/20 dilution (Lane 1). Lane 2 : PBS instead of K562 cell lysate. ab194829 at 1/1000 was used for subsequent western blot detection. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (ab194829)

Predicted band size: 51 kDa

false

Supplier Data

Western blot - Anti-TIM44 antibody [EPR16821] (AB194829)

All lanes:

Western blot - Anti-TIM44 antibody [EPR16821] (ab194829) at 1/5000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

Supplier Data

Western blot - Anti-TIM44 antibody [EPR16821] (AB194829)

All lanes:

Western blot - Anti-TIM44 antibody [EPR16821] (ab194829) at 1/1000 dilution

All lanes:

Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

false

Carrier free

Anti-TIM44 antibody [EPR16821] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16821

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TIM44 also known as translocase of inner mitochondrial membrane 44 serves an important role in mitochondrial protein import mechanisms. The protein weighs approximately 44 kDa and localizes in the inner mitochondrial membrane. TIM44 works as an essential part of the mitochondrial inner membrane presequence translocase complex where it assists in guiding proteins into the mitochondrial matrix. Expression of TIM44 is observed mainly in tissues with high energetic demands such as muscle and liver.

Biological function summary

TIM44 functions in conjunction with other components of the TIM23 complex which includes proteins like TIM23 and TIM17. The main role of TIM44 involves connecting the translocase complex to mitochondrial heat shock protein 70 (mtHsp70) facilitating ATP-dependent translocation of polypeptides. This process supports the import of precursor proteins synthesized in the cytosol into the mitochondria ensuring proper mitochondrial function and energy production.

Pathways

TIM44 influences the mitochondrial protein import pathway and the maintenance of mitochondrial function. As part of the protein import machinery it connects closely to the chaperone-mediated folding pathway aided by mtHsp70. The TIM23 complex participates in the translocation of proteins with mitochondrial targeting sequences linking TIM44 to pathways essential for mitochondrial biogenesis and function.

Dysfunction in the TIM44 protein associates with mitochondrial disorders including those impacting oxidative phosphorylation capacity. Mutations or deficiencies in TIM44 have connections to disorders such as mitochondrial myopathy. Relation to other proteins such as mtHsp70 and components of oxidative phosphorylation emphasizes the role of TIM44 in cellular energy homeostasis and highlights its importance in maintaining mitochondrial health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (By similarity). Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source (By similarity).

See full target information TIMM44

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1801 PubMed39979305

2025

Actin-dependent α-catenin oligomerization contributes to adherens junction assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Regina B Troyanovsky,Indrajyoti Indra,Sergey M Troyanovsky

Disease models & mechanisms 16: PubMed37283036

2023

Leader peptide or pro-segment mutants of renin are misrouted to mitochondria in autosomal dominant tubulointerstitial kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Céline Schaeffer,Maurizio De Fusco,Elena Pasqualetto,Caterina Scolari,Claudia Izzi,Francesco Scolari,Luca Rampoldi

Nature communications 13:1071 PubMed35228556

2022

Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shintaro Mise,Akinobu Matsumoto,Keisuke Shimada,Toshiaki Hosaka,Masatomo Takahashi,Kazuya Ichihara,Hideyuki Shimizu,Chisa Shiraishi,Daisuke Saito,Mikita Suyama,Tomoharu Yasuda,Toru Ide,Yoshihiro Izumi,Takeshi Bamba,Tomomi Kimura-Someya,Mikako Shirouzu,Haruhiko Miyata,Masahito Ikawa,Keiichi I Nakayama

Human molecular genetics 29:177-188 PubMed31868880

2019

Impaired turnover of hyperfused mitochondria in severe axonal neuropathy due to a novel DRP1 mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Fabiana Longo,Sara Benedetti,Alberto A Zambon,Maria Grazia Natali Sora,Chiara Di Resta,Daniele De Ritis,Angelo Quattrini,Francesca Maltecca,Maurizio Ferrari,Stefano Carlo Previtali

Cell reports 29:1728-1738.e9 PubMed31693908

2019

MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.

Applications

Unspecified application

Species

Unspecified reactive species

Jakob D Busch,Miriam Cipullo,Ilian Atanassov,Ana Bratic,Eduardo Silva Ramos,Thomas Schöndorf,Xinping Li,Sarah F Pearce,Dusanka Milenkovic,Joanna Rorbach,Nils-Göran Larsson

Molecular cell 74:701-712.e9 PubMed30948266

2019

Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Han Lee,Christine Mayr

Nature protocols 12:183-194 PubMed28005069

2016

Depletion of mitochondria in mammalian cells through enforced mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Correia-Melo,Gabriel Ichim,Stephen W G Tait,João F Passos

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIM44 antibody [EPR16821]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] (AB194829)

Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] (AB194829)

Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (AB194829)

Western blot - Anti-TIM44 antibody [EPR16821] (AB194829)

Western blot - Anti-TIM44 antibody [EPR16821] (AB194829)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise