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Rabbit Recombinant Monoclonal TIM44 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] (AB194829), expandable thumbnail
  • Western blot - Anti-TIM44 antibody [EPR16821] (AB194829), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] (AB194829), expandable thumbnail
  • Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (AB194829), expandable thumbnail
  • Western blot - Anti-TIM44 antibody [EPR16821] (AB194829), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/20
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner (By similarity). Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source (By similarity).

Alternative names

MIMT44, TIM44, TIMM44, Mitochondrial import inner membrane translocase subunit TIM44

Recommended products

Rabbit Recombinant Monoclonal TIM44 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16821
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TIM44 also known as translocase of inner mitochondrial membrane 44 serves an important role in mitochondrial protein import mechanisms. The protein weighs approximately 44 kDa and localizes in the inner mitochondrial membrane. TIM44 works as an essential part of the mitochondrial inner membrane presequence translocase complex where it assists in guiding proteins into the mitochondrial matrix. Expression of TIM44 is observed mainly in tissues with high energetic demands such as muscle and liver.

Biological function summary

TIM44 functions in conjunction with other components of the TIM23 complex which includes proteins like TIM23 and TIM17. The main role of TIM44 involves connecting the translocase complex to mitochondrial heat shock protein 70 (mtHsp70) facilitating ATP-dependent translocation of polypeptides. This process supports the import of precursor proteins synthesized in the cytosol into the mitochondria ensuring proper mitochondrial function and energy production.

Pathways

TIM44 influences the mitochondrial protein import pathway and the maintenance of mitochondrial function. As part of the protein import machinery it connects closely to the chaperone-mediated folding pathway aided by mtHsp70. The TIM23 complex participates in the translocation of proteins with mitochondrial targeting sequences linking TIM44 to pathways essential for mitochondrial biogenesis and function.

Associated diseases and disorders

Dysfunction in the TIM44 protein associates with mitochondrial disorders including those impacting oxidative phosphorylation capacity. Mutations or deficiencies in TIM44 have connections to disorders such as mitochondrial myopathy. Relation to other proteins such as mtHsp70 and components of oxidative phosphorylation emphasizes the role of TIM44 in cellular energy homeostasis and highlights its importance in maintaining mitochondrial health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] (ab194829), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] (ab194829)

    Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling TIM44 with ab194829 at 1/100 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (Goat Anti-Rabbit IgG H&L (HRP) ab97051). Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194829. Cytoplasm staining on humanendometrial adenocarcinoma was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-TIM44 antibody [EPR16821] (ab194829), expandable thumbnail

    Western blot - Anti-TIM44 antibody [EPR16821] (ab194829)

    All lanes: Western blot - Anti-TIM44 antibody [EPR16821] (ab194829) at 1/1000 dilution

    All lanes: Mouse heart tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] (ab194829), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] (ab194829)

    Immunofluorescent analysis of A431 cells labeling TIM44 with ab194829 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on A431 cell line was observed.

  • Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (ab194829), expandable thumbnail

    Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (ab194829)

    Immunoprecipitation analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using Anti-MRPL48 antibody [EPR16328] ab194826 at 1/20 dilution (Lane 1). Lane 2: PBS instead of K562 cell lysate. ab194829 at 1/1000 was used for subsequent western blot detection. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

    All lanes: Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (ab194829)

    Predicted band size: 51 kDa

  • Western blot - Anti-TIM44 antibody [EPR16821] (ab194829), expandable thumbnail

    Western blot - Anti-TIM44 antibody [EPR16821] (ab194829)

    All lanes: Western blot - Anti-TIM44 antibody [EPR16821] (ab194829) at 1/5000 dilution

    Lane 1: A431 cell lysate at 20 µg

    Lane 2: HepG2 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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